三皮素对抗痤疮的功效研究

doi:10.3969/j.issn.1001-1803.2019.01.007

摘要/Abstract

摘要：

以倒捻子果皮﹑厚朴树皮﹑石榴果皮3种植物皮提取物组成的三皮素溶液为研究对象,通过多种方法对其抗脂质过氧化﹑控油﹑抑菌﹑抗炎的功效进行研究。结果显示,三皮素在质量分数为1%时,对脂质过氧化有显著的抑制效果。质量分数为0.1%的三皮素处理皮脂腺细胞24 h后,可显著下调胰岛素样受体（IGF1R） ﹑过氧化物酶体增殖物激活受体（PPARγ）的mRNA表达水平来降低细胞内脂质合成。三皮素对痤疮丙酸杆菌的最低抑菌浓度（MIC）为0.03%。在痤疮丙酸杆菌诱导的巨噬细胞炎症模型上,三皮素能剂量依赖性地抑制白介素6 （IL6） ﹑前列腺素E2 （PGE2）和一氧化氮（NO）的表达。三皮素作为一种天然来源的活性原料,可针对痤疮发病机制中的各大因素来发挥其对抗痤疮的作用。

关键词: 化妆品添加剂, 三皮素, 痤疮, 抗炎

Abstract:

The solution of antisebum GMP which consists of the extracts from the peels of Garcinia Mangostana, Magnolia Officinalis, and Punica Granatum has been used to study its efficacy in anti-lipid peroxidation, oil control, anti-bacteria and anti-inflammation. The results show that GMP has significant inhibitory effect on lipid peroxidation at the mass concentration of 1%. After the treatment of sebaceous gland cells with GMP （0.1%） for 24 h, the mRNA expression levels of insulin like receptor （IGF1R） and peroxisome proliferator activated receptor （PPARγ） can be remarkably down-regulated to reduce the intracellular lipid synthesis in the cells. The minimum inhibitory concentration （MIC） of GMP on Propionibacterium acnes is 0.03%. On the model of macrophage inflammation induced by Propionibacterium acnes, GMP can also inhibit the expression of interleukin 6 （IL6）, prostaglandin E2 （PGE2） and nitric oxide （NO） in a dose-dependent manner. Therefore, as a natural active ingredient, GMP can play a role in anti-acne aiming at the various factors in the pathogenesis of acne vulgaris.

Key words: cosmetic additive, GMP, acne, anti-inflammatory

中图分类号:

TQ658.2

卢伊娜,王光寅,谢红,谢智勇. 三皮素对抗痤疮的功效研究[J]. 日用化学工业, 2019, 49(1): 29-33.

Yi-na LU,Guang-yin WANG,Hong XIE,Zhi-yong XIE. Efficacy study of antisebum GMP in the treatment of acne vulgaris[J]. China Surfactant Detergent & Cosmetics, 2019, 49(1): 29-33.

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参考文献 17

[1]	Fritsch M, Orfanos C E, Zouboulis C C . Sebocytes are the key regulators of androgen homeostasis in human skin[J]. Journal of Investigative Dermatology, 2001,116(5) : 793-800. doi: 10.1046/j.1523-1747.2001.01312.x pmid: 11348472
[2]	Makrantonaki E, Ganceviciene R, Zouboulis C . An update on the role of the sebaceous gland in the pathogenesis of acne[J]. Dermato-endocrinology, 2011,3(1) : 41-49. doi: 10.4161/derm.3.1.13900 pmid: 3051853
[3]	Wa I K, Danby F M, Qiang J , et al. New developments in our understanding of acne pathogenesis and treatment[J]. Experimental Dermatology, 2009,18(10) : 821-832. doi: 10.1111/j.1600-0625.2009.00890.x pmid: 19555434
[4]	Yi O S, Meyer A S, Frankel E N . Antioxidant activity of grape extracts in a lecithin liposome system[J]. Journal of the American Oil Chemists Society, 1997,74(10) : 1301-1307. doi: 10.1007/s11746-997-0061-9
[5]	Sato T, Imai N, Akimoto N , et al. Epidermal growth factor and 1α, 25-Dihydroxyvitamin D3 suppress lipogenesis in hamster sebaceous gland cells in vitro[J]. Journal of Investigative Dermatology, 2001,117(4) : 965-970. doi: 10.1046/j.0022-202x.2001.01516.x pmid: 11676839
[6]	Bai S B, Chen H X, Zhong J J , et al. RAW264.7 cell viability via MTT assay and possible factor analysis[J]. China Journal of Modern Medicine, 2011.
[7]	Kwon H H, Yoon J Y, Park S Y , et al. Activity-guided purification identifies lupeol, a pentacyclic triterpene, as a therapeutic agent multiple pathogenic factors of acne[J]. Journal of Investigative Dermatology, 2015,135(6) : 1491-1500. doi: 10.1038/jid.2015.29 pmid: 25647437
[8]	Lu Y N, Yang Y D, Xie Z Y . Promoting effect of Gmoist Sea-Gel on skin hydration and barrier function[J]. China Surfactant Detergent & Cosmetics, 2017,47(12) : 709-712.
[9]	Liu C H, Huang H Y . In vitro anti-propionibacterium activity by curcumin containing vesicle system[J]. Chemical & Pharmaceutical Bulletin, 2013,61(4) : 419-425. doi: 10.1248/cpb.c12-01043 pmid: 23546001
[10]	Karki R, Park C H, Kim D W . Extract of buckwheat sprouts scavenges oxidation and inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages (RAW264.7)[J]. Journal of Integrative Medicine, 2013,11(4) : 246-252. doi: 10.3736/jintegrmed2013036 pmid: 23867243
[11]	Yun K J, Kim J Y, Kim J B , et al. Inhibition of LPS-induced NO and PGE2 production by asiatic acid via NF-kB inactivation in RAW 264.7 macrophages: possible involvement of the IKK and MAPK pathways[J]. International Immunopharmacology, 2008,8(3) : 431-441. doi: 10.1016/j.intimp.2007.11.003
[12]	Thiboutot D . Regulation of human sebaceous glands[J]. Journal of Investigative Dermatology, 2004,123(1) : 1-12. doi: 10.1111/j.1523-1747.2004.t01-2-.x pmid: 15191536
[13]	Sato T, Takahashi A, Kojima M , et al. A citrus polymethoxy flavonoid, nobiletin inhibits sebum production and sebocyte proliferation, and augments sebum excretion in hamsters[J]. Journal of Investigative Dermatology, 2007,127(12) : 2740-2748. doi: 10.1038/sj.jid.5700927 pmid: 17597820
[14]	Schneider M R, Paus R . Sebocytes, multifaceted epithelial cells: Lipid production and holocrine secretion[J]. International Journal of Biochemistry & Cell Biology, 2010,42(2) : 181-185. doi: 10.1016/j.biocel.2009.11.017 pmid: 19944183
[15]	Beylot C, Auffret N, Poli F , et al. Propionibacterium acnes: an update on its role in the pathogenesis of acne[J]. Journal of the European Academy of Dermatology & Venereology, 2014,28(3) : 271-278.
[16]	Jappe U . Pathological mechanisms of acne with special emphasis on Propionibacterium acnes and related therapy[J]. Acta Derm. Venereol., 2003,83(4) : 241-248. doi: 10.1080/00015550310016463 pmid: 12926793
[17]	Vowels B R, Yang S, Leyden J J . Induction of proinflammatory cytokines by a soluble factor of Propionibacterium acnes: implications for chronic inflammatory acne[J]. Infection and Immunity, 1995,63(8) : 3158-3165. doi: 10.1016/1380-2933(95)00016-X pmid: 7542639

	w（三皮素）/%						w（Vc）/%
	0	0.1	0.3	1	3	10	1
A	1.108	0.967	0.622	0.583	0.387	0.365	0.194
抑制率/%	0.00	11.24^**	42.84^**	46.44^**	64.42^**	66.53^**	82.45^**

痤疮丙酸杆菌浓度× 10^-8/（CFU·mL^-1）	c（NO）/（μmol·L^-1）	ρ（IL6）/（ng·L^-1）	ρ（PGE2）/（ng·L^-1）
0	0.06	181.56	263.3
0.5	9.58^**	2 419.24^**	3 476.3^**