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日用化学工业（中英文）

日用化学工业 ›› 2018, Vol. 48 ›› Issue (1): 57-60.doi: 10.13218/j.cnki.csdc.2018.01.012

• 分析与检测 • 上一篇    

高效液相色谱法测定化妆品中氨甲环酸的含量

唐毓萍, 唐欣, 孙培文, 林艺青, 李征   

  1. 上海中翊日化有限公司,上海 200065
  • 收稿日期:2017-10-31 发布日期:2019-03-21
  • 作者简介:唐毓萍(1980-),女,工程师,硕士,电话:13918573047,E-mail:tangyuping@kans.cn。

Determination of the content of tranexamic acid in cosmetics by high performance liquid chromatography

TANG Yu-ping, TANG Xin, SUN Pei-wen, LIN Yi-qing, LI Zheng   

  1. Shanghai Zhongyi Cosmetic Co.,Ltd.,Shanghai 200065,China
  • Received:2017-10-31 Published:2019-03-21

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摘要： 建立了测定化妆品中氨甲环酸含量的高效液相色谱法。采用Zorbax SB C18色谱柱(250 mm×4.6 mm,5 μm)分离,以水-甲醇-18.3 g/L磷酸二氢钠溶液(含体积分数1.26%的三乙胺,pH=2.5)为流动相进行梯度洗脱,流速0.8 mL/min,柱温25 ℃,进样量10 μL,样品经体积比为1∶1的甲醇-饱和氯化钠溶液提取,用二极管阵列检测器(DAD)检测,检测波长220 nm,外标法定量。结果表明,氨甲环酸在100~4 000 mg/L范围内线性关系良好,相关系数R2＝0.999 9。方法的检出限为7 mg/L,定量限为22 mg/L。样品的平均加标回收率为94.0%~102.5%,相对标准偏差为0.57%~2.69% (n=3)。该方法前处理简单、回收率高、精密度好,适用于化妆品中氨甲环酸含量的测定。

关键词: 化妆品添加剂, 高效液相色谱, 氨甲环酸

Abstract: A high performance liquid chromatography was used to determine the content of tranexamic acid in cosmetics.The Zorbax SB C18 chromatographic column (250 mm×4.6 mm,5 μm) was used for separation,and water-methanol-18.3 g/L sodium dihydrogen phosphate solution (containing volume fraction 1.26% triethylamine and adjusted pH to 2.5 by phosphoric acid) was used as mobile phase for gradient elution,with a flow rate of 0.8 mL/min.Temperature of the column was 25 ℃ and the injection volume was 10 μL.Samples were extracted with methanol/saturated sodium chloride solution (volume ratio of 1∶1) and quantitated by diode array detector at 220 nm with external standard method.Results show that tranexamic acid has a good linear relationship in the range of 100-4 000 mg/L,and the correlation coefficient (R2) is 0.999 9.Limit of detection and quantitation of the method are 7 and 22 mg/L separately.The average spiked recoveries vary from 94.0% to 102.5%,and the relative standard deviations achieve 0.57%-2.69% (n=3).This method has the advantages of simple pretreatment,high recovery and good precision for the determination of the content of tranexamic acid in cosmetics.

Key words: additive of cosmetics, high performance liquid chromatography, tranexamic acid

中图分类号: 

  • TQ658