新型糖基阳离子表面活性剂与牛血清白蛋白的相互作用研究

doi:10.13218/j.cnki.csdc.2018.09.006

摘要/Abstract

摘要： 采用荧光光谱法和三维荧光光谱法研究了糖基阳离子表面活性剂N,N-二甲基-N[3-(糖酰胺基)]丙基-N-十四烷基溴化铵(C₁₄DGPB)与牛血清白蛋白(BSA)的相互作用。考察了各因素对C₁₄DGPB与BSA相互作用的影响。结果表明,当作用时间为30 min,NaCl的浓度为0.03 mol/L,pH=6.6时,为C₁₄DGPB与BSA相互作用的较佳条件;通过计算可以得到不同温度下C₁₄DGPB对BSA的动态猝灭常数、结合参数和热力学参数;由热力学参数确定它们之间的作用力主要是疏水作用力,对酪氨酸和色氨酸的疏水微环境影响较大。

关键词: 表面活性剂, 糖基阳离子, 牛血清白蛋白, 荧光光谱法, 三维荧光光谱法

Abstract: The interactions between a sugar-based cationic surfactant N,N-dimethyl-N-[3-(gluconamide)]propyl-N-tetradecylammonium bromides (C₁₄DGPB) and bovine serum albumin (BSA) were investigated by fluorescence and three-dimensional fluorescence spectroscopy.The effects of various factors on the interactions between C₁₄DGPB and BSA were investigated.The results show that the optimal conditions for interactions between C₁₄DGPB and BSA were the action time 30 min,0.03 mol/L NaCl and pH=6.6.The dynamic quenching constants,binding parameters and thermodynamic parameters of C₁₄DGPB with BSA at different temperatures can be obtained by calculation.According to the thermodynamic parameters,it shows that the hydrophobic interaction has played dominant roles in the interactions between C₁₄DGPB and BSA,which has a great influence on the hydrophobic microenvironment of tyrosine and tryptophan.

Key words: surfactant, carbohydrate cation, bovine serum albumin, fluorescence spectrometry, three-dimensional fluorescence spectrometry

中图分类号:

TQ423

智丽飞,王秀文,李晓明,陈慧,薛永兵,张清华. 新型糖基阳离子表面活性剂与牛血清白蛋白的相互作用研究[J]. 日用化学工业, 2018, 48(9): 511-515.

ZHI Li-fei,WANG Xiu-wen,LI Xiao-ming,CHEN Hui,XUE Yong-bing,ZHANG Qing-hua. Study on the interactions between a novel sugar-based cationic surfactant and bovine serum albumin[J]. China Surfactant Detergent & Cosmetics, 2018, 48(9): 511-515.

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