药用层孔菌发酵液控油及收缩毛孔机理及功效研究

doi:10.3969/j.issn.2097-2806.2024.05.010

摘要/Abstract

摘要：

将药用层孔菌和乳酸菌进行双向发酵得到药用层孔菌发酵液，检测药用层孔菌发酵液和药用层孔菌水提液的活性成分含量，从分子、细胞、动物和人体等方面研究药用层孔菌发酵液在控油和收缩毛孔等方面的功效作用，结果表明，药用层孔菌发酵液具有更高的活性成分含量、可以减少ABTS自由基的生成、抑制炎症因子TNF-α和IL-6的表达、调控5α还原酶活性、减少皮肤油脂分泌、促进Ⅰ型胶原蛋白（COL-Ⅰ）的表达、抑制金属蛋白酶-1（MMP-1）表达、具有收缩毛孔的趋势，具备成为具有控油和收缩毛孔功效的化妆品原料的潜力。

关键词: 药用层孔菌, 化妆品原料, 发酵技术, 控油功效, 收缩毛孔

Abstract:

The fermentation broth of Fomes officinalis was obtained by two-way fermentation of Fomes officinalis and Lactococcus lactis, and the contents of active components in the fermentation broth and aqueous extract were detected. The effects of Fomes officinalis fermentation broth on oil control and pore contraction were studied from the aspects of molecule, cell, animal and human body. The fermentation of Fomes officinalis has higher content of active components, can reduce the production of ABTS free radicals, inhibit the expression of inflammatory factors TNF-α and IL-6, regulate the activity of 5α reductase, reduce skin oil secretion, promote the expression of type Ⅰ collagen （COL-Ⅰ）, inhibit the expression of metalloproteinase-1 （MMP-1）, have the tendency of shrinking pores. It has the potential to become a cosmetic raw material with the effect of oil control and pore refining.

Key words: Fomes officinalis, raw material of cosmetics, fermentation technology, oil control effect, shrinking pores

中图分类号:

TQ658

宋阳, 吕永博, 任晗堃, 彭娇龙. 药用层孔菌发酵液控油及收缩毛孔机理及功效研究[J]. 日用化学工业（中英文）, 2024, 54(5): 566-573.

Yang Song, Yongbo Lv, Hankun Ren, Jiaolong Peng. Study on the mechanism and efficacy of controlling oil and shrinking pores in fermentation broth of Fomes officinalis[J]. China Surfactant Detergent & Cosmetics, 2024, 54(5): 566-573.

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添加试剂	受试样品	阳性物	50%乙醇	ABTS溶液
样品组	+	-	-	+
阳性对照组	-	+	-	+
阴性对照组	-	-	+	+
空白对照组	+	-	+	-

物质名称	加入量/μL	终浓度/（μmol/L）
5α-还原酶液	10	—
T	5	25
NADPH	10	100
待测药物	5
PBS	220

	总多糖含量/（mg/mL）	总甾醇含量/（mg/g）	总氨基酸含量/（mg/mL）
药用层孔菌发酵液	11.4	0.94	2.561
药用层孔菌水提液	1.49	0.32	0.251