高效液相色谱法同时测定化妆品原料甘草提取液中甘草苷和甘草酸

doi:10.3969/j.issn.1001-1803.2021.03.013

日用化学工业 ›› 2021, Vol. 51 ›› Issue (3): 250-255.doi: 10.3969/j.issn.1001-1803.2021.03.013

高效液相色谱法同时测定化妆品原料甘草提取液中甘草苷和甘草酸

杨红美¹(),徐嘉琪¹,龚盛昭^1,²(),余涓^1,³

1.广州环亚化妆品科技有限公司,广东广州 510663
2.广东轻工职业技术学院,广东广州 510300
3.广东环亚美容化妆品博物馆,广东广州 510663

收稿日期:2020-06-16 修回日期:2021-03-03 出版日期:2021-03-22 发布日期:2021-03-23
通讯作者: 龚盛昭
作者简介:杨红美（1990-）,女,分析研究员,硕士,电话：18675349627,E-mail：hongmeiapple19@163.com。
基金资助:
广州市科技计划项目资助(201604016111);广州市科学技术协会学术融合经费类资助(X20200501017)

Simultaneous determination of liquiritin and glycyrrhizic acid by high performance liquid chromatography in licorice extract in raw materials of cosmetics

YANG Hong-mei¹(),XU Jia-qi¹,GONG Sheng-zhao^1,²(),YU Juan^1,³

1. Guangzhou Uniasia Cosmetic Technology Co., Ltd., Guangzhou, Guangdong 510663, China
2. Guangdong Industry Technical College, Guangzhou, Guangdong 510300, China
3. Guangdong Huanya Beauty and Cosmetics Museum, Guangzhou, Guangdong 510663, China

Received:2020-06-16 Revised:2021-03-03 Online:2021-03-22 Published:2021-03-23
Contact: Sheng-zhao GONG

摘要/Abstract

摘要：

选取甘草提取液中典型有效成分甘草苷和甘草酸,建立其高效液相色谱检测方法。样品用质量分数为50%乙醇提取,以Agilent ZORBAX SB-C₁₈ （4.6 mm×250 mm×5 μm）为分析柱,乙腈-0.05%磷酸梯度洗脱,流速1.0 mL/min,检测波长237 nm。结果表明,该方法测试时间短,测试结果准确;在0.5~100 μg/mL范围内线性良好,相关系数均大于0.999 5; 空白样品中加标回收率为91.47%~114.57%,相对标准偏差为0.87%~3.32%。该方法准确、可靠,适用于化妆品原料甘草提取液中甘草苷和甘草酸的测定。

关键词: 化妆品原料, 甘草苷, 甘草酸, 高效液相色谱法

Abstract:

A comprehensive analytical method based on high performance liquid chromatography （HPLC） was developed for the determination of liquiritin and glycyrrhizic acid in licorice extract in cosmetics. The target compounds were extracted with 50% ethanol, separated on a Agilent ZORBAX SB-C₁₈ column （4.6 mm×250 mm×5 μm） in gradient elution program using acetonitrile and 0.05% phosphoric acid as mobile phases, at flow rate of 1 mL/min, and detection wavelength of 237 nm. The method had a short test time and accurate test results, it showed good linear relationship between 0.5-100 μg/mL with correlation coefficients larger than 0.999 5. The spiked recoveries in the blank sample were in the range of 91.47%-114.57% with the relative standard deviations of 0.87%-3.32%. The results indicate that the developed method is rapid, accurate and reliable, which is suitable for the determination of liquiritin and glycyrrhizic acid in licorice extract in cosmetics.

Key words: cosmetics raw materials, liquiritin, glycyrrhizic acid, high performance liquid chromatography （HPLC）

中图分类号:

TQ658

杨红美,徐嘉琪,龚盛昭,余涓. 高效液相色谱法同时测定化妆品原料甘草提取液中甘草苷和甘草酸[J]. 日用化学工业, 2021, 51(3): 250-255.

YANG Hong-mei,XU Jia-qi,GONG Sheng-zhao,YU Juan. Simultaneous determination of liquiritin and glycyrrhizic acid by high performance liquid chromatography in licorice extract in raw materials of cosmetics[J]. China Surfactant Detergent & Cosmetics, 2021, 51(3): 250-255.

图/表 7

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参考文献 13

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组分	线性范围/（μg·mL^-1）	线性回归方程	相关系数（r²）
甘草苷	0.5~100	y=14.759x+3.810	0. 999 9
甘草酸	0.5~100	y=5.583x+1.168	0. 999 8

	加入量/（μg·mL^-1）	实测值/（μg·mL^-1）	加标回收率/%	平均回收率/%	RSD/%
甘草苷	1	1.15	115	111.50	3.25
		1.16	116
		1.11	111
		1.08	108
		1.07	107	111.50	3.25
		1.12	112	111.50	3.25
甘草苷	10	11.55	115.5	114.57	1.54
		11.45	114.5
		11.63	116.3
		11.60	116.0
		11.17	111.7
		11.34	113.4
	20	22.21	111.05	112.38	0.87
		22.48	112.40
		22.58	112.90
		22.63	113.15
		22.27	111.35
		22.68	113.40
甘草酸	1	0.95	95	92.17	3.32
		0.97	97
		0.90	90
		0.91	91
		0.90	90
		0.90	90
	10	9.20	92.0	91.47	1.43
		9.0	90.0
		9.0	90.0
		9.34	93.4
		9.19	91.9
		9.15	91.5
	20	19.34	96.70	97.66	1.96
		19.63	98.15
		19.61	98.05
		19.79	98.95
		19.95	99.75
		18.87	94.35

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高效液相色谱法同时测定化妆品原料甘草提取液中甘草苷和甘草酸

Simultaneous determination of liquiritin and glycyrrhizic acid by high performance liquid chromatography in licorice extract in raw materials of cosmetics

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