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日用化学工业(中英文) ›› 2022, Vol. 52 ›› Issue (10): 1072-1080.doi: 10.3969/j.issn.1001-1803.2022.10.006

• 开发与应用 • 上一篇    下一篇

虫草素纳米乳液制备及对皮肤光老化修复机制研究

张虎成*(),杨国伟,杨军,范海涛,罗帅,刘霖颖   

  1. 北京电子科技职业学院 生物工程学院,北京 100176
  • 收稿日期:2022-05-23 修回日期:2022-09-28 出版日期:2022-10-22 发布日期:2022-10-24
  • 通讯作者: 张虎成
  • 基金资助:
    基金北京电子科技职业学院校级重大课题(2022X002-KXD);北京市教委面上项目(2018Z004-003-KWY)

Preparation of cordycepin nanoemulsion and its repair mechanism on the photoaged skin

Zhang Hucheng*(),Yang Guowei,Yang Jun,Fan Haitao,Luo Shuai,Liu Linying   

  1. College of Biological Engineering, Beijing Polytechnic, Beijing 100176, China
  • Received:2022-05-23 Revised:2022-09-28 Online:2022-10-22 Published:2022-10-24
  • Contact: Hucheng Zhang

摘要:

将虫草素(cordycepin)作为功效成分制作成纳米乳液,并研究了虫草素纳米乳液配方工艺、质量初步评价和对皮肤光老化修复机制。通过绘制伪三元相图,确定虫草素纳米乳液配方,考察纳米乳粒径、黏度、稳定性,以及虫草素稳定性。利用紫外线照射新西兰兔子皮肤,检测皮肤中的超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)活力和丙二醛(MDA)的含量。结果表明,乳化剂和助乳化剂质量比(Km)为2∶1时得到的纳米乳液较稳定,虫草素纳米乳平均直径为374 nm,黏度为(9.33±0.14) mPa·s,具有良好的离心稳定性、稀释稳定性、长期稳定性和温度稳定性;虫草素1~8个月维持在(12.006±0.025) mg/g,RSD在1%以内,第9个月开始减少;使用虫草素纳米乳液实验组4的SOD、CAT活性和GSH含量稍低于对照组,显著高于实验组1、2和3 (P<0.01),对照组和实验组4的MDA含量均明显低于实验组1、2和3 (P<0.01)。虫草素纳米乳液具有较好的稳定性、黏度和粒径大小,可以有效缓解紫外线照射引起的皮肤衰老。

关键词: 虫草素, 纳米乳液, 高效液相色谱, 抗衰老, 稳定性

Abstract:

Cordycepin was used as an effective component to prepare nanoemulsion. The formulation process, preliminary quality evaluation and skin photoaging repair mechanism of cordycepin nanoemulsion were studied. By drawing the pseudo ternary phase diagram, the formula of the nanoemulsion was determined. The particle size, viscosity, and stability of the nanoemulsion were investigated. The activity of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) and the content of malondialdehyde (MDA) in the skin of New Zealand rabbits were measured. The results show that the nanoemulsion is stable when the Km of surfactant and co-surfactant is 2∶1. The average diameter of cordycepin nanoemulsion is 374 nm and the viscosity is (9.33±0.14) mPa·s. It has good centrifugal stability, dilution stability, long-term stability and temperature stability. Cordycepin can be maintained at (12.006±0.025) mg/g for 1-8 months with the RSD within 1%, and it begins to decrease in the ninth month. The activities of SOD, CAT and GSH in group 4 using cordycepin nanoemulsion are slightly lower than those in the control group, significantly higher than those in groups 1, 2 and 3 (P<0.01). The MDA in the control group and group 4 are significantly lower than those in groups 1, 2 and 3 (P<0.01). Cordycepin nanoemulsion has good stability, viscosity and particle size, and can effectively alleviate skin aging caused by ultraviolet radiation. This study aims to lay a theoretical foundation and provide direction for the further development of the function of cordycepin nanoemulsion.

Key words: cordycepin, nanoemulsion, high performance liquid chromatography, anti-aging, stability

中图分类号: 

  • TQ658