复合活性物促进毛发生长作用的研究

doi:10.3969/j.issn.1001-1803.2022.01.008

摘要/Abstract

摘要：

以黄芪多糖为主要原料制备成复合活性物,通过体外细胞和动物实验测定复合活性物的抗炎及促进毛发生长活性,并初步分析其作用机制。结果显示,本实验方法制备的黄芪多糖含量为86.21%,动物实验结果显示复合活性物显著增加小鼠脱发区皮肤的毛囊数目和真皮层厚度（p<0.05）,延长毛发生长期的时间,其作用机制可能是通过下调TGF-β1、IL-1β的表达延缓毛囊进入休止期,同时上调皮肤组织中VEGF的表达,促进毛发生长功效。此外,通过体外细胞实验结果发现复合活性物、黄芪多糖在设定浓度下为实际无细胞毒性,同时对脂多糖（LPS）诱导的NO、IL-1β具有显著呈浓度依赖性抑制作用,提示复合活性物及黄芪多糖具有良好的抗炎活性。进一步发现,复合活性物对抑制LPS诱导RAW264.7细胞产生的IL-1β分泌效果,显著缩短脱毛小鼠皮肤长出毛发的时间、增加真皮厚度以及抑制TGF-β1表达,均优于单一黄芪多糖,说明复合活性物组分可能存在协同增效作用。

关键词: 复合活性物, 黄芪多糖, 多肽, 毛发生长, 抗炎

Abstract:

Astragalus polysaccharide （APS） was used as the main raw material to prepare the Complex active substance （CAS）. The anti-inflammatory and hair-growth promoting activities of CAS were determined by in vitro cell and animal experiments, and the mechanism of action was preliminarily analyzed. The results showed that the content of APS prepared by this method was 86.21%. The results of animal experiment showed that the CAS significantly increased the number of hair follicles and the thickness of the dermis in the alopecia area of mice （p<0.05）, and prolonged the hair growth period. The mechanism might be that the expression of TGF-β1 and IL-1β could be down-regulated to delay hair follicles entering the resting period, and the expression of VEGF in skin tissue could be up-regulated to promote hair-follicle angiogenesis, improve nutrient absorption of hair follicles and thus play a role in promoting hair growth. In addition, the results of in vitro cell experiments showed that the CAS and APS had no cytotoxicity at the investigated concentration, and showed significant concentration-dependent inhibitory effects on LPS-induced NO and IL-1β, suggesting that CAS and APS had good anti-inflammatory activity. Compared with Astragalus polysaccharide, CAS could significantly shorten the time taking for hair growth on the hair-removed skin in mice, and could significantly increase the thickness of the dermis and the number of follicles. Furthermore, CAS could significantly inhibit TGF-β1 expression compared with APS. The animal experiments confirmed that the CAS could inhibit inflammation factor and promote hair growth, which was better than single Astragalus polysaccharides. Moreover, the components of the CAS might have synergistic effects. In conclusion, the CAS could effectively inhibit the inflammatory reaction and promote hair growth, which could be used as active ingredients in daily cosmetics with functions of relieving skin inflammation, preventing hair loss, hair conditioning and hair care.

Key words: Complex active substance, Astragalus polysaccharide, polypeptide, hair growth, anti-inflammatory

中图分类号:

TQ658

李洪海,韩萍,马诗经,向琴,汤仲标,杜志云. 复合活性物促进毛发生长作用的研究[J]. 日用化学工业, 2022, 52(1): 50-55.

Li Honghai,Han Ping,Ma Shijing,Xiang Qin,Tang Zhongbiao,Du Zhiyun. Study on the hair-growth promotion effect of Complex active substance[J]. China Surfactant Detergent & Cosmetics, 2022, 52(1): 50-55.

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实验组	ρ/（μg/mL）	细胞活性/%	毒性分级
空白对照组	—	100.00±0.10	0
黄芪多糖	500	94.18±0.23	1
黄芪多糖	1 000	93.05±0.48	1
复合多肽（4 μg/mL^@）	500	98.86±0.16	1
复合多肽（4 μg/mL^@）	1 000	96.21±0.36	1
复合活性物	10	101.49±0.41	0
	50	98.85±1.23	1
	250	97.83±0.19	1
	500	99.22±0.23	1
	1 000	93.06±0.81	1

实验组	ρ/ （μg/mL）	NO含量/ （μmol/L）	IL-1β含量/ （pg/mg）
对照组	—	13.92±0.51	9.89±2.26
模型组（LPS）	1（LPS）	40.22±0.18^###	50.24±1.12
黄芪多糖	500	22.23±0.22^***	27.57±2.17^***
黄芪多糖	1 000	20.59±0.41^***	25.82±3.21^***
复合活性物	10	38.87±0.38	47.93±2.76
	50	35.96±0.11^*	45.19±3.82
	250	28.03±0.57^**	34.02±1.19^**
	500	22.87±0.33^***	26.38±1.28^***
	1 000	21.94±0.43^***	23.05±0.99^***

组别	T1皮肤变灰时间/d	T2皮肤长出毛发时间/d
空白对照组	11.4±1.35	15.2±1.91
阳性对照组	7.2±1.44^*	10.1±2.58^*
黄芪多糖组	8.6±1.16^*	12.5±1.29
复合活性物组	7.4±2.76^*	11.3±1.38^*

实验组	真皮厚度/μm	毛囊数量/个
空白对照组	375.5±26.5	57.5±13.2
阳性对照组	456.5±49.9^*	89.5±15.1^*
黄芪多糖组	421.7±21.0	87.0±16.2^*
复合活性物组	439.5±26.8^*	98.9±12.1^**

组别	TGF-β1含量/ （pg/mg）	IL-1β含量/ （pg/mg）	VEGF含量/ （pg/mg）
空白对照组	112.08±2.11	39.65±1.73	45.94±0.04
阳性组	105.77±2.72	32.14±2.93	67.61±2.34^**
黄芪多糖组	95.97±1.86	30.27±1.02^*	50.86±0.98^*
复合活性物组	49.58±1.73^***	23.27±3.69^**	61.33±1.74^**