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日用化学工业（中英文）

日用化学工业 ›› 2022, Vol. 52 ›› Issue (1): 44-49.doi: 10.3969/j.issn.1001-1803.2022.01.007

• 开发与应用 • 上一篇    下一篇

茶麸黄酮促进毛乳头细胞增殖和VEGF分泌的研究

沈胡驰1,陈殿松2,杨井国1,2,王靖1,2,*()   

  1. 1.江南大学 化学与材料工程学院 化妆品研究中心,江苏 无锡 214122
    2.阿道夫科研创新实验室 广州德谷个人护理用品有限公司,广东 广州 510000
  • 收稿日期:2021-04-13 修回日期:2021-12-27 出版日期:2022-01-22 发布日期:2022-01-19
  • 通讯作者: 王靖
  • 基金资助:
    国家自然科学基金资助项目(51802123)

Study on the promotion of proliferation of dermal papilla cells and VEGF secretion by flavonoids from camellia seed cake

Shen Huchi1,Chen Diansong2,Yang Jingguo1,2,Wang Jing1,2,*()   

  1. 1. Cosmetic Research Center, School of Chemistry and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China
    2. Guangzhou Degu Personal Care Products Co., Ltd., Adolf Research Innovation Laboratory, Guangzhou, Guangdong 510000, China
  • Received:2021-04-13 Revised:2021-12-27 Online:2022-01-22 Published:2022-01-19
  • Contact: Jing Wang

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摘要：

以脱脂茶麸为原料,通过乙醇水溶液超声波辅助提取脱脂茶麸中的黄酮,探究其生物活性。用不同质量浓度含茶麸黄酮溶液作用于体外培养的人毛乳头细胞（HDPCs）,四甲基偶氮唑蓝（MTT）法测定细胞活性,并通过流式细胞术检测茶麸黄酮对细胞凋亡、周期的影响以及ELISA法检测细胞培养上清液中血管内皮生长因子（VEGF）的变化。结果表明,茶麸黄酮富集产物对HDPCs具有增殖作用,质量浓度20 μg/mL时增殖效果最佳,比10 μg/mL米诺地尔阳性对照效果显著（**P<0.01）。茶麸中黄酮可能是通过减少HDPCs的早凋,促进HDPCs的有丝分裂从而对细胞的增殖发生作用。茶麸黄酮富集产物对HDPCs的VEGF分泌呈现浓度依赖性。

关键词: 茶麸, 黄酮, 毛乳头细胞, 细胞周期, 血管内皮生长因子

Abstract:

Defatted camellia seed cake was used as raw material, and the flavonoids therein were extracted with aqueous ethanol solution with the aid of ultrasonic wave to explore their biological activity. Different mass concentrations of camellia seed cake flavonoids were applied to human dermal papilla cells （HDPCs） cultured in vitro, and the cell viability was determined by MTT method. The effects on apoptosis and cell cycle were detected by flow cytometry. ELISA method was used to detect the change of vascular endothelial growth factor （VEGF） in cell culture supernatant. The results showed that flavonoid-enriched product of camellia seed cake had a proliferation effect on HDPCs and could promote the secretion of VEGF in a concentration-dependent manner. The proliferation effect was the best when the concentration of flavonoid-enriched product was 20 μg/mL, which was more effective than the positive control of 10 μg/mL minoxidil （**P<0.01）. The flavonoids in camellia seed cake might reduce the premature apoptosis of HDPCs and promote the mitosis of HDPCs and thus lead to an effect on their proliferation.

Key words: camellia seed cake, flavonoid, human dermal papilla cell, cell cycle, vascular endothelial growth factor

中图分类号: 

  • TQ658