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日用化学工业（中英文）

日用化学工业 ›› 2017, Vol. 47 ›› Issue (2): 90-95.doi: 10.13218/j.cnki.csdc.2017.02.007

• 开发与应用 • 上一篇    下一篇

皮肤致敏测试动物替代方法DPRA的建立和应用

王滢, 吴越   

  1. 伽蓝集团股份有限公司 研发中心,上海 200233
  • 收稿日期:2016-08-29 出版日期:2017-02-22 发布日期:2019-03-19
  • 通讯作者: 吴 越,博士,电话:(021)62635551,E-mail:wuyue@jala.com.cn。
  • 作者简介:王 滢(1985-),女,博士,电话:(021)22275670,E-mail:wangying6@jala.com.cn。   

Establishment and application of non-animal test method for skin sensitization (DPRA)

WANG Ying, WU Yue   

  1. R&D Center,JALA Corporation,Shanghai 200233,China
  • Received:2016-08-29 Online:2017-02-22 Published:2019-03-19

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摘要： 皮肤致敏是化妆品原料安全性评估中的一项重要内容。按照ECVAM (European Centre for the Validation of Alternative Methods)的标准,通过对16个标准品进行检测,成功建立了DPRA (Direct Peptide Reaction Assay,直接肽链反应分析)实验方法。其检测结果的敏感性,特异性,准确性,S/NS的判定以及4 Class分别为81.82%,100%,77.8%,100%和94.7%。目前该方法已成功运用在实际工作中,并检测出多款具有引起皮肤致敏潜在能力的化妆品原料。

关键词: 化妆品原料, 皮肤致敏, 动物替代方法, 直接肽链反应分析

Abstract: The identification of the skin sensitization potential is an important component of the safety assessment of cosmetic ingredients.A DPRA (Direct Peptide Reaction Assay) test method according to the specifications of ECVAM (European Centre for the Validation of Alternative Methods) was established through detection of 16 kinds of standard cosmetic ingredient.Results showed that the established DPRA test method meets the specifications of ECVAM and its sensitivity,specificity and accuracy achieves 81.82%,100%,77.8% respectively.Meanwhile,for the laboratory reproducibility,the accuracy for S/NS and 4 Class classifications achieves 100% and 94.7% respectively.Now,the method has been applied in practice and several kinds of material used for formulating cosmetic products have been detected showing skin sensitization potential.

Key words: cosmetic ingredient, skin sensitization, non-animal test method, Direct Peptide Reaction Assay

中图分类号: 

  • TQ658