乳酸促脂溢性脱发小鼠毛发生长研究

doi:10.3969/j.issn.2097-2806.2023.09.005

摘要/Abstract

摘要：

通过除去C57BL/6小鼠背部毛发及涂抹睾酮建立脂溢性脱发模型，研究乳酸对脂溢性脱发小鼠毛发生长，毛囊生长周期及生长因子的影响。将60只C57BL/6小鼠随机分为正常组，模型组，米诺地尔酊阳性组，乳酸高、中、低剂量组，并分别在脱毛区涂抹相对应的药物。连续给药24天，记录小鼠皮肤颜色变化时间、脱毛区毛发生长状况以及毛囊组织学特征和毛囊数目的变化；使用Elisa试剂盒检测小鼠体内KGF，VEGF和TGF-β1的质量浓度。结果表明，与模型组、正常组相比较，乳酸能显著促进脂溢性脱发小鼠毛发生长，增加小鼠毛发重量和给药实验区域皮肤毛囊数量，同时上调小鼠皮肤中KGF和VEGF质量浓度，降低小鼠皮肤中TGF-β1质量浓度，差异有统计学意义（P<0.05），其中以中剂量10 g/L乳酸溶液效果最佳，可加快小鼠毛发生长、促进小鼠毛囊生长期转化进程，抑制脂溢性脱发症状，其疗效与阳性药米诺地尔酊效果相当（P>0.05），可制成日用洗护产品预防脱发，为脂溢性脱发患者提供更多选择。

关键词: 脂溢性脱发, 乳酸, 毛发生长, 角质形成细胞生长因子, 毛囊细胞

Abstract:

The seborrheic alopecia model was established by removing the back hair of C57BL/6 mice and smearing with testosterone. The effects of lactic acid on hair growth, hair follicle growth cycle and growth factors in seborrheic alopecia mice were studied based on this model. Sixty C57BL/6 mice were randomly divided into normal group, model group, minoxidil tincture positive group, and groups of high-, medium- and low-dose lactic acid, which were smeared with corresponding drugs on the depilation area. After 24 days of continual administration, the time of skin color change, the hair growth in depilation area, the histological characteristics of hair follicles and the change of hair follicle number were recorded. The concentration of KGF, VEGF, and TGF-β1 in mouse skin was measured by corresponding Elisa kits. The results showed that, compared with the model group and normal group, lactic acid could significantly promote hair elongation in seborrheic alopecia mice, and increase the weight of mouse hair and the number of skin hair follicles in the drug-administration experimental area. Meanwhile, the concentrations of KGF and VEGF in mouse skin were up-regulated, and the concentration of TGF-β1 in mouse skin was reduced, both of which the differences were statistically significant （P<0.05）. The medium dose （10 g/L） of lactic acid solution had the best effect, which could accelerate the hair growth of mice, promote the transformation process of hair follicles in the growth period, and inhibit the symptoms of seborrheic alopecia. The curative effect of medium dose （10 g/L） of lactic acid solution was equivalent to that of minoxidil tincture （P>0.05）. It could be applied in daily care products to prevent alopecia and provide more choice for patients with seborrheic alopecia.

Key words: seborrheic alopecia, lactic acid, hair growth, keratinocyte growth factor, hair follicle cell

中图分类号:

TQ658

罗晓健, 洪石希, 罗晶, 杨思, 王紫艳, 熊耀坤. 乳酸促脂溢性脱发小鼠毛发生长研究[J]. 日用化学工业（中英文）, 2023, 53(9): 1029-1034.

Luo Xiaojian, Hong Shixi, Luo Jing, Yang Si, Wang Ziyan, Xiong Yaokun. Study on the effect of lactic acid in promoting hair growth in seborrheic alopecia mice[J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1029-1034.

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参考文献 21

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组别	t/d
正常组	7.2 ±0.92^*
米诺地尔酊组	7.4 ±0.87^*
脂溢性脱发模型组	8.25±1.17^▲
5 g/L乳酸溶液组	7.6 ±0.89
10 g/L乳酸溶液组	7.7 ±0.71
20 g/L乳酸溶液组	7.6 ±0.74

组别	第15天新生毛发长度/mm	第24天新生毛发长度/mm
正常组	5.40±0.23^*	10.47±0.19^**
米诺地尔酊组	5.01±0.95	10.26±0.82^**
脂溢性脱发模型组	4.71±0.75	8.75±1.07^▲
5 g/L乳酸溶液组	5.55±0.26^*	9.90±0.64^*
10 g/L乳酸溶液组	5.83±0.72^**	10.02±0.71^*
20 g/L乳酸溶液组	5.31±0.69^*	9.26±1.26

组别	小鼠新生毛发重量/mg
正常组	60.2±4.8^**
米诺地尔酊组	57.2±6.3^**
脂溢性脱发模型组	46.1±4.0 ^▲
5 g/L乳酸溶液组	44.5±3.4^▲
10 g/L乳酸溶液组	58.5±3.8^**
20 g/L乳酸溶液组	51.6±5.2^*

组别	平均毛囊数量/（个/视野）
正常组	27.80±4.12^**
米诺地尔酊组	25.33±2.37^*
脂溢性脱发模型组	19.27±3.74^▲
5 g/L乳酸溶液组	21.60±2.93
10 g/L乳酸溶液组	27.73±4.53^*
20 g/L乳酸溶液组	23.00±0.47

组别	ρ （KGF） /（×10^-6 g/L）	ρ （VEGF） /（×10^-6 g/L）	ρ （TGF-β1） /（×10^-6 g/L）
正常组	1.00±0.10^**	1.37±0.18^**	97±55^**
米诺地尔酊组	0.97±0.13^*	1.23±0.30^**	142±28^*
脂溢性脱发模型组	0.75±0.09^▲	0.77±0.11^▲	240±47^▲
5 g/L乳酸溶液组	0.81±0.18	0.87±0.10	173±30^*
10 g/L乳酸溶液组	0.96±0.08^**	1.09±0.20^**	180±17^*
20 g/L乳酸溶液组	0.81±0.21	0.84±0.11	175±22^*