消毒剂对重组Ⅲ型人源化胶原蛋白促细胞黏附活性的影响

doi:10.3969/j.issn.2097-2806.2023.07.008

日用化学工业（中英文） ›› 2023, Vol. 53 ›› Issue (7): 789-895.doi: 10.3969/j.issn.2097-2806.2023.07.008

消毒剂对重组Ⅲ型人源化胶原蛋白促细胞黏附活性的影响

李怡桐¹,孙艳艳²,乔绍俊²,侯丽莉²,李熳琴³,于玉凤^3,^*()

1.中国海洋大学海德学院，山东青岛 266100
2.山西锦波生物医药股份有限公司功能蛋白山西省重点实验室，山西太原 030032
3.山西医科大学基础医学院，山西太原 030001

收稿日期:2023-04-11 修回日期:2023-06-27 出版日期:2023-07-22 发布日期:2023-07-25
基金资助:
山西省重点研发计划资助项目(201903D311008)

Effect of disinfectants on cell adhesion activity of recombinant type Ⅲ humanized collagen

Li Yitong¹,Sun Yanyan²,Qiao Shaojun²,Hou Lili²,Li Manqin³,Yu Yufeng^3,^*()

1. Haide College, Ocean University of China, Qingdao, Shandong 266100, China
2. Shanxi Key Laboratory of Functional Proteins, Shanxi Jinbo Bio-Pharmaceutical Co., Ltd., Taiyuan, Shanxi 030032, China
3. College of Basic Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi 030001, China

Received:2023-04-11 Revised:2023-06-27 Online:2023-07-22 Published:2023-07-25
Contact: *Tel.: +86-18512519265, E-mail: yuyf021@sxmu.edu.cn.

摘要/Abstract

摘要：

通过CCK8实验探究75%乙醇、碘伏、葡萄糖酸洗必泰、苯扎氯铵、苯扎溴铵对细胞的毒性以及对重组Ⅲ型人源化胶原蛋白（rhCol Ⅲ）促细胞的黏附活性的影响，预测消毒剂的使用在面部填充时对胶原蛋白活性的影响，为临床应用提供指导。结果显示：各消毒剂对NIH/3T3小鼠胚胎成纤维细胞和HFB人皮肤成纤维细胞均表现出细胞毒性，比较结果为：葡萄糖酸洗必泰<苯扎氯铵、苯扎溴铵<75%乙醇、碘伏，该结果也间接提示75%乙醇、碘伏消毒效果较佳；将各消毒剂原液稀释100倍后，其对rhCol Ⅲ促细胞黏附活性影响比较为：葡萄糖酸洗必泰、苯扎氯铵<75%乙醇<碘伏、苯扎溴铵。碘伏和苯扎溴铵工作液直接接触rhCol Ⅲ均显著降低其促细胞黏附活性，碘伏、葡萄糖酸洗必泰、苯扎溴铵工作液直接接触rhCol Ⅲ可导致其发生聚集。

关键词: 重组人源化胶原蛋白, 填充, 消毒剂, 细胞毒性, 细胞黏附活性

Abstract:

The disinfectants available for facial injectable fillers include 75% ethanol, iodine-containing and surfactant-based disinfectants. There is a lack of research on whether the use of various skin disinfectants influences the activity of recombinant type Ⅲ humanized collagen （rhCol Ⅲ）. The toxicity of 75% ethanol, iodophor disinfectant, chlorhexidine gluconate, benzalkonium chloride, and benzalkonium bromide on cells and the cell adhesion activity of rhCol Ⅲ were investigated by CCK8 experiments to predict the effect of disinfectant on collagen activity during facial fillers, in order to provide guidance for clinical application. The results show that all disinfectants have cytotoxicity to NIH/3T3 mouse embryonic fibroblasts and HFB human skin fibroblasts, and the comparative results rank as chlorhexidine gluconate

Key words: recombinant humanized collagen, filler, disinfectant, cytotoxicity, cell adhesion activity

中图分类号:

TQ658

李怡桐, 孙艳艳, 乔绍俊, 侯丽莉, 李熳琴, 于玉凤. 消毒剂对重组Ⅲ型人源化胶原蛋白促细胞黏附活性的影响[J]. 日用化学工业（中英文）, 2023, 53(7): 789-895.

Li Yitong, Sun Yanyan, Qiao Shaojun, Hou Lili, Li Manqin, Yu Yufeng. Effect of disinfectants on cell adhesion activity of recombinant type Ⅲ humanized collagen[J]. China Surfactant Detergent & Cosmetics, 2023, 53(7): 789-895.

图/表 6

图 1

图 2

图 3

图 4

图 5

图 6

参考文献 16

[1]	Zhou Dan, Yin Yiqiu, Shen Yanqin, et al. Research progress on modification of collagen[J]. China Dyeing & Finishing, 2022, 48 (10) : 78-81.
[2]	Shang Jixian. Clinical observation of 15 patients with facial atrophic concave acne-like scar corrected by collagen injection[J]. Shanxi Medical Journal, 2021, 50 (9) : 1525-1527.
[3]	Yang Jianqiu, Gao Yihong, Hongliu Zhu. Effect and mechanism of recombinant human type Ⅲ collagen functional gel on skin wound healing[J]. Shandong Medical Journal, 2021, 61 (34) : 80-82.
[4]	National Medical products Administration. Recombinant type Ⅲ humanized collagen lyophilized fiber approved for marketing[EB/OL]. National Medical products Administration. 2021: XZXK-2021-205. https://www.nmpa.gov.cn/yaowen/ypjgyw/20210629141635133.html.
[5]	Wang Yunbing, Li Gaocan, Yang Li, et al. Development of innovative biomaterials and devices for the treatment of cardiovascular diseases[J]. Adv. Mater., 2022, 34 (46).
[6]	Wang J, Qiu H, Xu Y, et al. The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging: An in vivo study[J]. Bioact. Mater., 2022, 11: 154-165.
[7]	You Shuang, Zhu Yun, Li Hu, et al. Recombinant humanized collagen remodels endometrial immune microenvironment of chronic endometritis through macrophage immunomodulation[J]. Regen Biomater, 2023, 10.
[8]	Shuai Xinxing, Kang Ning, Li Yuhan, et al. Recombination humanized type Ⅲ collagen promotes oral ulcer healing[J]. Oral Dis, 2023.
[9]	Yang Na, Yang Bin, Sun Wenkui, et al. Progress in development and application of skin disinfectants[J]. Chinese Journal of Disinfection, 2018, 35 (4) : 297-300.
[10]	Aziz Zumrat, Zhang Yumei, He Congfen, et al. Types and protection recommendations of disinfectants[J]. Deterg. Cosmet., 2020, 43 (7) : 53-59.
[11]	Jacobsen G, Rasmussen K, Bregnhøj A, et al. Causes of irritant contact dermatitis after occupational skin exposure: a systematic review[J]. Int. Arch. Occup. Environ. Health, 2022, 95 (1) : 35-65. doi: 10.1007/s00420-021-01781-0
[12]	Goh C F, Ming L C, Wong L C. Dermatologic reactions to disinfectant use during the COVID-19 pandemic[J]. Clinics in Dermatology, 2021, 39 (2) : 314-322. doi: 10.1016/j.clindermatol.2020.09.005
[13]	Xiao Hao, Zhang Hongting, Zhang Li, et al. Status of research on chlorhexidine allergy[J]. Chinese Journal of Allergy & Clinical, 2020, 14 (5) : 465-470.
[14]	Chen Xuejian, Guo Zhengyang, Huang Jianfei, et al. research progress on quaternary ammonium salt disinfectant[J]. Guangdong Chemical Industry, 2019, 46 (16) : 240-241.
[15]	Maillard J Y. Bacterial target sites for biocide action[J]. J. Appl. Microbiol., 2002, 92: 16-27.
[16]	Hua Chen, Zhu Yun, Xu Wei, et al. Characterization by high-resolution crystal structure analysis of a triple-helix region of human collagen type Ⅲ with potent cell adhesion activity[J]. Biochem. Biophys. Res. Commun., 2019, 508 (4) : 1018-1023. doi: 10.1016/j.bbrc.2018.12.018

消毒剂对重组Ⅲ型人源化胶原蛋白促细胞黏附活性的影响

Effect of disinfectants on cell adhesion activity of recombinant type Ⅲ humanized collagen

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 16

相关文章 5

Metrics

本文评价

推荐阅读 10

[1]	王展印,高文军,卫罡,张争明. 过硫酸氢钾复合物消毒剂的制备及杀菌性能研究[J]. 日用化学工业, 2019, 49(8): 515-518.
[2]	周龙梅,张亮亮,王金燕. 高稳定二溴海因水溶液制备及其消毒效果研究[J]. 日用化学工业, 2019, 49(1): 40-43.
[3]	梁勇坤,郭丽娟,王北明. 曙红Y分光光度法测定季铵盐消毒剂的含量分析方法研究[J]. 日用化学工业, 2018, 48(10): 601-604.
[4]	朱洲海, 刘凤至, 黄海涛, 张涛, 米其利, 管莹, 夭建华. 几种儿童牙膏体外细胞毒性研究[J]. 日用化学工业, 2015, 45(5): 282-284.
[5]	李燕, 陈建英, 王晓梅, 刘霞, 陈倩倩, 凌沛学. 硅烷化玻璃酸酯的制备及其护肤功效研究[J]. 日用化学工业, 2014, 44(9): 497-500.