几种儿童牙膏体外细胞毒性研究

doi:10.13218/j.cnki.csdc.2015.05.010

日用化学工业 ›› 2015, Vol. 45 ›› Issue (5): 282-284.doi: 10.13218/j.cnki.csdc.2015.05.010

几种儿童牙膏体外细胞毒性研究

朱洲海¹, 刘凤至², 黄海涛¹, 张涛¹, 米其利¹, 管莹¹, 夭建华¹

1.云南中烟工业有限责任公司技术中心,云南昆明 650106;
2.云南中医学院,云南昆明 650500

发布日期:2019-04-02
通讯作者: 夭建华,研究员,硕士,E-mail:jhyao_2007@126.com。
作者简介:朱洲海(1982-),助理研究员,博士,E-mail:zhuzhouhai@gmail.com。
基金资助:
中国烟草总公司资助项目(2011B086);云南中烟工业有限责任公司资助项目(2012JC04)

In vitro cytotoxicity study on several kinds of toothpaste for children

ZHU Zhou-hai¹, LIU Feng-zhi², HUANG Hai-tao¹, ZHANG Tao¹, MI Qi-li¹, GUAN Ying¹, YAO Jian-hua¹

1.Technology Center of China Tobacco Yunnan Industrial Co.,Ltd.,Kunming,Yunnan 650106,China;
2.Yunnan University of TCM,Kunming,Yunnan 650500,China

Published:2019-04-02

摘要/Abstract

摘要： 使用体外培养的人口腔黏膜成纤维细胞,通过MTT法,测定了几种市售普通儿童牙膏和可吞咽儿童牙膏浸提液的细胞相对增殖率(RGR),并进行细胞毒性分级,同时与成人牙膏的细胞毒性进行比较,从而反映这些牙膏对口腔黏膜的影响。结果表明:可吞咽儿童牙膏和普通儿童牙膏的细胞毒性相对较小;成人牙膏细胞毒性较大,与儿童牙膏比较细胞相对增殖率有显著性差异(P<0.05),可能会对儿童的口腔黏膜造成刺激。

关键词: 儿童牙膏, 可吞咽牙膏, 口腔黏膜细胞, 细胞毒性

Abstract: In vitro cultivated human oral mucosal fibroblasts were used to determine the relative growth rate (RGR) and cytotoxicity grade of extract of several kinds of common children toothpaste and swallow-able children toothpaste via MTT method,and compared with that of adult toothpaste.It reflected the influence of the toothpaste to oral mucosa.Results showed that,the cytotoxicity of swallow-able children toothpaste and common children toothpaste is relatively lower than that of adult toothpaste.There are significant difference between the adult toothpaste and children toothpastes on cell growth rate (P<0.05).Cytotoxicity of adult toothpaste is relatively higher,and may cause irritation to the oral mucosa of children.

Key words: toothpaste for children, swallow-able toothpaste, oral mucosa cells, cytotoxicity

中图分类号:

TQ658.4+1

朱洲海, 刘凤至, 黄海涛, 张涛, 米其利, 管莹, 夭建华. 几种儿童牙膏体外细胞毒性研究[J]. 日用化学工业, 2015, 45(5): 282-284.

ZHU Zhou-hai, LIU Feng-zhi, HUANG Hai-tao, ZHANG Tao, MI Qi-li, GUAN Ying, YAO Jian-hua. In vitro cytotoxicity study on several kinds of toothpaste for children[J]. China Surfactant Detergent & Cosmetics, 2015, 45(5): 282-284.

参考文献

[1] 李伟年.儿童牙膏市场及儿童牙齿的护理[J].日用化学品科学,2006,29(12):6-8.
[2] 周文森.牙膏中的单氟磷酸钠和氟化钠比较[J].日用化学工业,1998(5):33-35.
[3] 高鹰,张秋霞,刘萍.对牙膏安全性评价的思考[J].日用化学品科学,2010,33(7):7-10.
[4] WRIGHT J T,HANSON N,RISTIC H,et al.Fluoride toothpaste efficacy and safety in children younger than 6 years:A systematic review [J].J Am Dent Assoc,2014,145(2):182-189.
[5] AMERICAN DENTAL ASSOCIATION COUNCIL ON SCIENTIFIC AFFAIRS.Fluoride toothpaste use for young children [J].J Am Dent Assoc,2014,145(2):190-191.
[6] BUZALAF M A,CARDOSO CDE A,MAGALHÃES A C.Low-fluoride toothpastes may not lead to dental fluorosis but may not control caries development.Standard fluoride toothpastes can control caries development but may lead to dental fluorosis [J].J Evid Based Dent Pract,2013,13(4):148-150.
[7] SANTOS A P,OLIVEIRA B H,NADANOVSKY P.Effects of low and standard fluoride toothpastes on caries and fluorosis:Systematic review and meta-analysis [J].Caries Res,2013,47(5):382-390.
[8] 葛敏.浅谈含氟牙膏对学龄前儿童的影响[J].牙膏工业,2004(3):26-27.
[9] 周静,冯希平,束陈斌.牙膏氟含量对学龄前儿童氟摄入影响的研究[J].现代口腔医学杂志,2002(4):366-367.
[10] GERCKENS B,EISINGER G,KADEN P,et al.Comparative studies of toothpastes and toothpaste ingredients in biological systems:1.Can various toothpastes be differentiated by relative biological effectiveness in cell culture studies? [J].Oralprophylaxe,1991,13(2):55-60.
[11] 章香娇,章培标,朱国庆,等.牙膏浸出物细胞毒性及其检测方法研究[J].中国公共卫生,2002(10):59-60.
[12] 刘克明,张耀亭,周蕾,等.口腔卫生用品的安全性评价[J].卫生毒理学杂志,2004(2):125-126.
[13] 刘克明,支秀珍,李新.20种含药物牙膏的致突变检测及分析[J].中国公共卫生,1994(8):362-363.
[14] 刘克明,辛显桐,支秀珍,等.牙膏的毒理学检验及评价[J].中国公共卫生,2001(11):25.
[15] USPC.Biological test/biological reactivity test,in vitro [J].Rockville:USP,2004.

几种儿童牙膏体外细胞毒性研究

In vitro cytotoxicity study on several kinds of toothpaste for children

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