中药双向发酵液制备及其抗衰老、保湿、美白功效研究

摘要/Abstract

图/表 22

参考文献 18

doi:10.3969/j.issn.2097-2806.2023.05.005

摘要：

通过对中国灵芝、铁皮石斛、黄精、晚香玉进行双向发酵工艺优化，筛选制备出最优发酵液，利用不同质量分数中药双向发酵液对体外抑制蛋白质非酶糖基化、酪氨酸酶活性抑制、水通道蛋白Aquaporin3（AQP3）含量、2，2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除率、1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除率、小鼠黑素B16-F10细胞黑色素含量进行测定。研究结果发现，中药双向发酵液能够显著抑制蛋白质非酶糖基化、酪氨酸酶活性、小鼠B16-F10细胞黑色素含量（P<0.05），并且能够显著清除DPPH和ABTS自由基，促进角质形成细胞AQP3蛋白表达（P<0.01），这为化妆品原料的开发提供了新的方向和数据依据。

关键词: 双向发酵, 蛋白非酶糖基化, 酪氨酸酶, 自由基, 水通道蛋白AQP3

Abstract:

In this study, the bi-directional fermentation process of Chinese Ganoderma lucidum, Dendrobium officinale, Polygonatum chinensis and Tuberose was optimized, and the optimal fermentation broth was prepared. Activity inhibition, Aquaporin3 （AQP3） content, 2, 2’-diazo-bis-3-ethylbenzothiazoline-6-sulfonic acid （ABTS） free radical scavenging rate, 1, 1-diphenyl-2-trinitrophenylhydrazine （DPPH） free radical scavenging rate and melanin content in mouse melanin B16-F10 cells were measured. The results of the study find that the bi-directional fermentation broth of traditional Chinese medicine can significantly inhibit protein non-enzymatic glycosylation, tyrosinase activity, and melanin content in mouse B16-F10 cells （P<0.05）. It can also significantly scavenge DPPH and ABTS free radicals, and upregulate AQP3 protein expression in keratinocytes （P<0.01）. Therefore, this paper concludes that the bi-directional fermentation broth of traditional Chinese medicine screened by optimizing the bi-directional fermentation process has a significant effect on inhibiting protein non-enzymatic glycosylation, tyrosinase activity and melanin content of mouse melanin B16-F10 cells, upregulating AQP3 protein expression, and scavenging ABTS free radicals and DPPH free radicals in vitro. It provides a new direction and basis for the development of cosmetic raw materials.

Key words: bi-directional fermentation, protein non-enzymatic glycosylation, tyrosinase, free radicals, aquaporin AQP3

中图分类号:

TQ658

郭芳钰, 韩婷婷, 王晓娜, 陈玉荣, 王晓梅, 杨素珍. 中药双向发酵液制备及其抗衰老、保湿、美白功效研究[J]. 日用化学工业（中英文）, 2023, 53(5): 523-531.

Guo Fangyu, Han Tingting, Wang Xiaona, Chen Yurong, Wang Xiaomei, Yang Suzhen. Process optimization and anti-aging, moisturizing and whitening effects of traditional Chinese medicine bi-directional fermentation broth[J]. China Surfactant Detergent & Cosmetics, 2023, 53(5): 523-531.

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pH 7.4缓冲溶液/mL

0.1%酪氨酸溶液/mL

0.3 g/mL酪氨酸酶溶液/mL

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样品编号	碳源（w/%）	氮源（w/%）	无机盐（w/%）	样品编号	碳源（w/%）	氮源（w/%）	无机盐（w/%）
1	1%葡萄糖	0.5%蛋白胨	0.5% NaCl，0.02% CaCO₃	4	1%蔗糖	0.5%酵母粉	0.5% NaCl，0.02% CaCO₃
2	1%葡萄糖	0.5%酵母粉		5	1%淀粉	0.5%蛋白胨
3	1%蔗糖	0.5%蛋白胨		6	1%淀粉	0.5%酵母粉

样品编号	w/%
样品编号	淀粉	酵母粉	NaCl	CaCO₃
1	1	0.5	0.3	0.02
2	1	1	0.5
3	1	1.5	0.7
4	2	0.5	0.5
5	2	1	0.7
6	2	1.5	0.3
7	3	0.5	0.7
8	3	1	0.3
9	3	1.5	0.5

序号	组别	信息		检测系统
序号	组别	样品名称	样品量	检测项目	检测模型	检测方法
组1	阴性对照组	SC		AQP3	Keratinocyte	IF
组1	样品组	中药双向发酵液	0.05%（w/%）	AQP3	Keratinocyte	IF
组2	阴性对照组	SC（1% DMSO）		AQP3	Keratinocyte	IF
组2	阳性对照组	维甲酸	2.5 μmol/L	AQP3	Keratinocyte	IF

添加试剂	受试样品/mL	50%乙醇/mL	ABTS溶液/mL
样品组	0.5	0	0.5
阳性对照组	0.5	0	0.5
阴性对照组	0	0.5	0.5
空白对照组	0.5	0.5	0