一种酿酒酵母和副干酪乳杆菌发酵产物滤液复合物对面部微生态的影响

doi:10.3969/j.issn.2097-2806.2023.11.001

摘要/Abstract

摘要：

本研究旨在考察一种经喜马拉雅食品来源酿酒酵母和副干酪乳杆菌发酵而得的滤液复合物（SLFC）对面部细菌微生态和皮肤屏障的调节作用。筛选有皮肤衰老迹象女性30人（30~45岁，平均年龄38岁），对比使用SLFC前（D0）和使用28天后（D28）两组样本，面部细菌物种Alpha多样性显著降低（p<0.05）；Beta多样性分组有意义（p<0.05）；在ASV（amplicon sequence variants）水平上考察物种组成，结果显示葡萄球菌属丰度显著增加（p<0.05），丙酸杆菌属、棒状杆菌属、不动杆菌属等皮肤常驻菌的相对丰度保持稳定，链球菌属、红球菌属、水栖菌属、黄杆菌属等机会致病菌的丰度下降。对皮肤特征菌检测，结果显示表皮葡萄球菌的丰度显著提高（p<0.05）；痤疮丙酸杆菌丰度变化不显著；金黄色葡萄球菌则无检出。D28组消费者反馈肌肤平滑、光泽和保湿状态得到改善，此外肌肤泛红得到缓解。由此可得，发酵产物复合物SLFC能有效调节并稳定面部菌群微生态平衡，巩固皮肤屏障。

关键词: 面部, 皮肤, 屏障, 微生态, 发酵

Abstract:

The objective of this study was to investigate the effects of a Himalaya-derived Saccharomyces and Lactobacillus ferment complex （SLFC） on the facial micro-ecology. Thirty women （30-45 years old, average 38 years old） with signs of skin aging used SLFC. Compared with the two groups of samples before use （D0） and after 28 days of use （D28）, the Alpha diversity of facial bacterial species decreased significantly and the Beta diversity had obvious differentiation in different regions. The relative abundance of Staphylococcus increased significantly. The relative abundance of Propionibacterium, Corynebacterium, Acinetobacter, and other skin resident bacteria remained stable. The relative abundance of the opportunistic pathogenic bacteria such as Streptococcus,Erythrococcus, Aquatic bacteria and flavobacterium decreased. At the species level, the abundance of Staphylococcus epidermidis increased significantly, the abundance of Cutibacterium acnes did not change significantly, and Staphylococcus aureus was not detected. According to the feedback of consumers after treatment with SLFC for 28 days, skin smoothness, gloss and moisture were improved and the skin redness was relieved. Therefore, SLFC can effectively regulate and stabilize the microecological balance of facial flora to improve and strengthen the skin barrier.

Key words: face, skin, barrier, micro-ecology, fermentation

中图分类号:

TQ658

王玥, 李俊, 顾仕红, 王曼, 邹岳, 吴建铭. 一种酿酒酵母和副干酪乳杆菌发酵产物滤液复合物对面部微生态的影响[J]. 日用化学工业（中英文）, 2023, 53(11): 1241-1249.

Wang Yue, Li Jun, Gu Shihong, Wang Man, Zou Yue, Wu Jianming. Effects of a Saccharomyces cerevisiae and Lactobacillus paracasei ferment complex on the facial micro-ecology[J]. China Surfactant Detergent & Cosmetics, 2023, 53(11): 1241-1249.

图/表 9

参考文献 19

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Target	Sequence （5′-3′）
16S	338F：ACTCCTACGGGAGGCAGCAG
16S	806R：GGACTACHVGGGTWTCTAAT

Species	Target	Sequence （5′-3′）
Cutibacterium acnes （C. acnes）	16S	PA1-129F: 5′- GACTTTGGGATAACTTCAGGAAACTG-3′
		PA1-238R: 5′- CTGATAAGCCGCGAGTCCAT-3′
		PA1-195P: 5′-TTGGAAAGTTTCGGCGGTTG-3′
Staphylococcus epidermidis （S. epidermidis）	atlE	atlE_1610F: 5′- GGAGGAACTAATAATAAGTTAACTG-3′
		atlE_1702R: 5′- GTCATAAACAGTTGTATATAAGCC-3′
		atlE_1638P: 5′- CTGCTAATCGTGGTGTTGCTCAAATTAAA-3′
Staphylococcus aureus （S. aureus）	nuc	SAF: 5′-GCAAAGAAAATTGAAGTCGAGTT-3′
		SAR: 5′-AAATATACGCTAAGCCACGTCCAT-3′
		SAP: 5′-ACAAAGGTCAAAGAACTGATA-3′

Characteristic Bacteria	Microbial abundance/（copies/μL）
Characteristic Bacteria	D0（M±SD）	D28（M±SD）
C. acnes	（2.34±4.40） ×10⁷	（2.37±2.83） ×10⁷（p>0.05）
S. epidermidis	（1.17±2.18） ×10⁵	（3.64±8.29） ×10⁵（p<0.05）

Number	Question	W0			W4
Number	Question	Agree	Disagree	P	Agree	Disagree	P
1	Has the delicacy of your skin improved?	26	4	0.000	30	0	0.000
2	Has the smoothness of your skin improved?	24	6	0.001	29	1	0.000
3	Has the luster of your skin improved?	29	1	0.000	30	0	0.000
4	Has the elasticity of your skin improved?	19	11	0.051	27	3	0.000
5	Has the firmness of your skin improved?	23	7	0.002	27	3	0.000
6	Have the fine lines on your skin improved?	24	6	0.001	24	6	0.001
7	Has the dullness of your skin improved?	19	11	0.051	23	7	0.002
8	Has the yellowness of your skin improved?	19	11	0.051	24	6	0.001
9	Has the redness of your skin improved?	8	22	0.005	29	1	0.000
10	Has the sensitivity of your skin improved?	9	21	0.013	29	1	0.000