当归提取物对毛发生长作用的体外与体内研究

doi:10.3969/j.issn.1001-1803.2020.12.009

日用化学工业 ›› 2020, Vol. 50 ›› Issue (12): 867-874.doi: 10.3969/j.issn.1001-1803.2020.12.009

当归提取物对毛发生长作用的体外与体内研究

高合意¹(),雷登凤²,程树军³,陈志杰⁴,刘仁龙¹()

1.重庆大学,重庆 401331
2.广东艾圣日用化学品有限公司,广东广州 510460
3.上海交通大学医学院公共卫生学院,上海 200025
4.广州市华代生物科技有限公司,广东广州 510535

收稿日期:2020-11-06 修回日期:2020-12-01 出版日期:2020-12-22 发布日期:2020-12-22
通讯作者: 刘仁龙
作者简介:高合意（1982-）,男,湖北黄冈人,博士研究生,主要研究方向为植物活性原料制备及机理研究,E-mail： heygao@163.com。
基金资助:
NSFC—云南联合基金(U1802255);广州市开发区国际科技合作项目(2017GH11)

Study on the effects of Angelica sinensis on hair growth in vitro and in vivo

GAO He-yi¹(),LEI Deng-feng²,CHENG Shu-jun³,CHEN Zhi-jie⁴,LIU Ren-long¹()

1. Chongqing University, Chongqing 401331, China
2. Guangdong Essence Daily Chemical Co., Ltd., Guangzhou, Guangdong 510460, China
3. School of Public Health, School of Medicine, Shanghai Jiao Tong University,Shanghai 200025, China
4. Guangzhou Chn-Alternative Biotechnology Co., Ltd., Guangzhou, Guangdong 510535, China

Received:2020-11-06 Revised:2020-12-01 Online:2020-12-22 Published:2020-12-22
Contact: Ren-long LIU

摘要/Abstract

摘要：

研究当归提取物在防脱育发功效中的作用。制备当归水提取物,采用Ⅱ型5α-还原酶抑制模型、C57BL/6小鼠动物实验模型和人群试用方法,分别进行酶抑制率、动物皮肤形态组织学观察和临床观察。结果表明0.5 g/mL的当归提取物可明显抑制Ⅱ型5α-还原酶的活性;动物实验结果表明,当归提取物对C57BL/6小鼠毛发生长有明显促进作用,刺激小鼠毛囊从休止期进入生长期;人体功效试验结果表明,使用当归提取物制备的头皮护理精华液7周后,80%志愿者的脱发症状得到改善。综合体外和体内研究结果表明：当归提取物具有促进小鼠毛发生长、改善脱发人群脱发症状的功效。

关键词: 当归提取物, 5α-还原酶, 毛发生长

Abstract:

The effects of the extract of Angelica sinensis on anti-hair loss and hair growth were studied. The efficacy of the extract of Angelica sinensis was evaluated by using different methods such as the model of 5α-reductase inhibition, animal test and human efficacy test. The results showed that this material could inhibit the activity of 5α-reductase in vitro with a dose of 0.5 g/mL. Animal test showed that it could obviously promote hair growth of C57BL/6 mice, and it could induce the hair follicle to enter from telogen into anagen. Human efficacy test showed that the hair loss symptoms could be reduced after using the scalp care essence containing the extract of Angelica sinensis for 7 weeks. In conclusion, the extract of Angelica sinensis could stimulate the hair growth of C57BL/6 mice, and it could help the patients with hair loss symptoms.

Key words: Angelica sinensis, 5α-reductase, hair growth

中图分类号:

TQ658

高合意,雷登凤,程树军,陈志杰,刘仁龙. 当归提取物对毛发生长作用的体外与体内研究[J]. 日用化学工业, 2020, 50(12): 867-874.

GAO He-yi,LEI Deng-feng,CHENG Shu-jun,CHEN Zhi-jie,LIU Ren-long. Study on the effects of Angelica sinensis on hair growth in vitro and in vivo[J]. China Surfactant Detergent & Cosmetics, 2020, 50(12): 867-874.

图/表 11

表 1

表 2

图 1

表 3

表 4

图 2

表 5

图 3

表 6

图 4

图 5

参考文献 17

[1]	Song Jiquan, Liu Jing, Liu Shengwu, et al. Effect of angelica polysaccharide on human scalp hair growth in vitro[J]. China Journal of Leprosy and Skin Diseases, 2007 (11) : 960-962.
[2]	Sun Shuang, Wang Yuhong, Zhang Fujuan, et al. Clinical observation and analysis of ginger angelica lotion[J]. Journal of North Pharmacy, 2013,10(2) : 14-15.
[3]	Kim Mi Hye, Choi You Yeon, Cho Ik-Hyun, et al. Angelica sinensis induces hair regrowth via the inhibition of apoptosis signaling[J]. The American Journal of Chinese Medicine, 2014,42(4) : 1021-1034. doi: 10.1142/S0192415X14500645 pmid: 25004889
[4]	Tae-Kyeong Lee, Bora Kim, Dae Won Kim, et al. Effects of decursin and angelica gigas nakai root extract on hair growth in mouse dorsal skin via regulating inflammatory cytokines[J]. Molecules, 2020,25(16) : 3697. doi: 10.3390/molecules25163697
[5]	Yang Zhuangqun, Tu Junbo, Yao Tian, et al. Effects of NGF and estrogens on human hair follicle in vitro[J]. Chinese Journal of Plastic Surgery, 2004 (1) : 47-49. pmid: 12778797
[6]	Sun Yu. Tu Ping, Wu Ling-shen, et al. Effect of Chinese herb medicines on hair growth in vivo[J]. Journal of Clincal Dekimaiology, 2005,34(3) : 147-150.
[7]	Yi Xingping, Xia Longqing. The role of 5α-reductase in androgen metabolism and its application in dermatology[J]. Foreign Medical Sciences Section of Dermatology and Venereology, 2004 (6) : 375-377.
[8]	Kim E J, Kim M K, Jeon Y J, et al. The promoting effect of Ishige sinicola on hair growth[J]. Marine Drugs, 2013,11(6) : 1783-1799. pmid: 23708185
[9]	Juan Zhao, Naoaki Harada, Kenji Okajima. Dihydrotestosterone inhibits hair growth in mice by inhibiting insulin-like growth factor-I production in dermal papillae[J]. Growth Hormone & IGF Research, 2011,21:260-267. doi: 10.1016/j.ghir.2011.07.003 pmid: 21839661
[10]	Naphatsorn Kumara, Wandee Rungseevijitprapab, Nual-Anong Narkkhongl, et al. 5α- reductase inhibition and hair growth promotion of some Thai plants traditionally used for hair treatment[J]. Journal of Ethnopharmacology, 2012,139:765-771. pmid: 22178180
[11]	Elizabeth M Wilson. Analysis of interdomain interactions of the androgen receptor[J]. Methods Mol Biol, 2011,776:113-129. doi: 10.1007/978-1-61779-243-4_8 pmid: 21796524
[12]	Sawaya M E. Purification of androgen receptors in human sebocytes and hair[J]. The Journal of Investigative Dermatology, 1992,98(6S) : 92S-96S. doi: 10.1111/1523-1747.ep12462313
[13]	He Hongmei, Zhu Hongxia, Liu Qiang, et al. Influence of the extract of polygoni multiflori radix on follicle growth and hair growth of C57BL/6[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012,18(23) : 216-219.
[14]	Zhu Hongxia, Liu Li, Zhang Lu, et al. Influence of the extract of polygoni multiflori radix on hair growth of C57BL/6J and its mechanism[J]. Journal of Chinese Medicinal Materials, 2012,35(10) : 1665-1667.
[15]	Liu Li, Zhu Hongxia, Chen Yuyao, et al. Influence of rhizoma zingiberis recens on hair growth of C57BL/6[J]. Journal of Liaoning University of Traditional Chinese, 2013,15(7) : 42-44.
[16]	Gao Heyi, Huang Jiancong, Lei Dengfeng, et al. Study on preperation and efficacy evaluation of herbal compound material for hair grow[J]. China Surfactant Detergent & Cosmetics, 2018,48(9) : 521-526.
[17]	Han Huixun, Ma Yunshu, Cui Lili, et al. Percutaneous penetration effect of three kinds of traditional Chinese medicine volatile oil on tetramethylpyrazine phosphate[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012,18(8) : 1-4.

项目	名称	w/%
A相	去离子水	至100
	丙二醇	4
	1,3-丁二醇	5
	汉生胶	0.1
B相	当归提取液（生药质量浓度1 g/mL）	50
C相	1,2-己二醇	0.5
C相	苯氧乙醇	0.5

项目	指标
菌落总数/（CFU·mL^-1）	≤1 000
霉菌、酵母菌/（CFU·mL^-1）	≤100
大肠杆菌/mL	不得检出
绿脓杆菌/mL	不得检出
金黄色葡萄球菌/mL	不得检出
汞/（mg·kg^-1）	≤1
铅/（mg·kg^-1）	≤10
砷/（mg·kg^-1）	≤2
镉/（mg·kg^-1）	≤5

组别	皮肤由粉红到灰的时间/d	灰色皮肤到长出毛的时间/d
空白对照组	18.80±1.94	25.40±1.02
25%样品组	20.20±2.48	25.40±2.33
50%样品组	21.40±4.22	25.60±4.08
100%样品组	14.60±2.87^*	20.40±2.58^*
阳性对照组	12.60±4.03^*	20.40±3.26^*

组别	当归提取物^*		何首乌提取物^[14]		生姜汁^[15]		复配原料^[16]
组别	皮肤变灰时间/d	长毛发时间/d	皮肤变灰时间/d	长毛发时间/d	皮肤变灰时间/d	长毛发时间/d	皮肤变灰时间/d	长毛发时间/d
空白对照组	17~20	24~26	14~16	19~21	14~16	19~21	14~22	20~28
阳性对照	8~16	17~23	7~10	13~17	7~10	12~14	7~12	9~15
高剂量样品	12~17	18~23	8~10	12~16	9~12	15~17	10~14	14~18
中剂量样品	17~26	21~30	12~15	13~17	9~14	16~19	10~19	15~24
低剂量样品	17~23	23~28	12~16	16~19	12~15	17~19	19~29	23~29

指标	空白对照组	阳性对照组	25%样品组	50%样品组	100%样品组
毛囊总数/个	350.00±35.36	445.83±36.80^*	245.83±36.80	308.33±30.28	437.50±46.77^*
新生毛囊数量/个	33.33±12.91	108.33±25.82^*	29.17±10.21	58.33±12.91^*	95.83±18.82^*
新生系数	0.09±0.03	0.24±0.06^*	0.12±0.03	0.19±0.05^*	0.22±0.02^*

当归提取物对毛发生长作用的体外与体内研究

Study on the effects of Angelica sinensis on hair growth in vitro and in vivo

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 17

相关文章 6

Metrics

本文评价

推荐阅读 10

组别	受试人数	观察时间/h	斑贴试验反应人数
组别	受试人数	观察时间/h	0级	1级	2级	3级	4级
头皮护理精华液	34	0.5	34	0	0	0	0
	34	24	34	0	0	0	0
	34	48	34	0	0	0	0
空白对照	34	0.5	33	1（31#）	0	0	0
	34	24	34	0	0	0	0
	34	48	34	0	0	0	0

[1]	罗晓健, 洪石希, 罗晶, 杨思, 王紫艳, 熊耀坤. 乳酸促脂溢性脱发小鼠毛发生长研究[J]. 日用化学工业（中英文）, 2023, 53(9): 1029-1034.
[2]	马铃, 沈胡驰, 陈殿松, 杨井国, 王靖. 超声辅助酶法提取茶麸黄酮及其对Ⅱ型5α-还原酶抑制活性[J]. 日用化学工业（中英文）, 2023, 53(3): 308-315.
[3]	李洪海,韩萍,马诗经,向琴,汤仲标,杜志云. 复合活性物促进毛发生长作用的研究[J]. 日用化学工业, 2022, 52(1): 50-55.
[4]	赵大敏,王学东,翟春涛. 祛痘中草药的筛选及其在治疗痤疮中的应用[J]. 日用化学工业, 2021, 51(6): 522-527.
[5]	李蕊,张楠,姚雷,李玉红. 薰衣草精油和亚洲薄荷精油促毛发生长作用的研究[J]. 日用化学工业, 2020, 50(8): 536-541.
[6]	高合意, 黄健聪, 雷登凤, 陈志杰, 梁钦智, 曾飒. 复配植物防脱生发原料的制备与功效评价研究[J]. 日用化学工业, 2018, 48(9): 521-526.