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日用化学工业（中英文）

日用化学工业 ›› 2016, Vol. 46 ›› Issue (1): 44-47.doi: 10.13218/j.cnki.csdc.2016.01.009

• 开发与应用 • 上一篇    下一篇

柯里拉京对B16细胞增殖及酪氨酸酶活性的抑制作用

黄海潮,郑公铭,王如意,张显策,李忠军,刘纲勇,傅中   

  1. 广东食品药品职业学院,广东 广州 510520
  • 出版日期:2016-01-22 发布日期:2016-01-22
  • 通讯作者: 郑公铭,教授,博士,E-mail:zhenggm@gdyzy.edu.cn。
  • 作者简介:黄海潮(1982-),男,广东惠州人,实验师,硕士,E-mail:huanghac@gdyzy.edu.cn。
  • 基金资助:
    广东省科技计划资助项目(2010B020312006);广东省中医药管理局2012年建设中医药强省科研资助项目(20121105)

Inhibition of corilagin on the proliferation of B16 melanoma cells and tyrosinase activity

HUANG Hai-chao,ZHENG Gong-ming,WANG Ru-yi,ZHANG Xian-ce,LI Zhong-jun,LIU Gang-yong,FU Zhong   

  1. Guangdong Food and Drug Vocational College,Guangzhou,Guangdong 510520,China
  • Online:2016-01-22 Published:2016-01-22

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摘要： 采用体外培养B16细胞,在柯里拉京作用不同时间后,用MTT(四甲基偶氮唑蓝)法检测柯里拉京对B16细胞生长的影响;用多巴氧化法检测柯里拉京对B16细胞中酪氨酸酶活性的抑制作用;用形态学和Hoechst33258染色法观察柯里拉京对B16细胞生长形态的影响。结果表明,柯里拉京对B16细胞增殖的抑制作用明显,其作用12,24和48 h的IC50分别是35.63,25.78和20.94 mg/L;对作用于B16细胞中酪氨酸酶12,24和48 h时的IC50分别是16.38,14.00和13.85 mg/L;形态学观察表明,当ρ(柯里拉京)＞16 mg/L作用24 h时,B16细胞损伤明显;Hoechst33258染色显示,当ρ(柯里拉京)≤16 mg/L作用24 h时,B16细胞呈现凋亡形态。

关键词: 化妆品添加剂, 柯里拉京, B16细胞, 酪氨酸酶

Abstract: B16 melanoma cells were cultivated in vitro.After the B16 melanoma cells were treated by corilagin for different time periods,the effect of corilagin on growth rate of proliferation of B16 cells was detected by methyl thiazolyl tetrazolium (MTT) assay and inhibition of corilagin on tyrosinase activity in B16 cells was detected by Dopa oxidation.Effect of corilagin on B16 cell morphology was detected with morphological observation and Hoechst33258 staining.Results showed that proliferation of B16 cells treated for 12,24 and 48 h with corilagin was inhibited with IC50 at 35.63,25.78 and 20.94 mg/L respectively;tyrosinase activity in the B16 cells treated for 12,24 and 48 h with corilagin was inhibited with IC50 at 16.38,14.00 and 13.85 mg/L respectively;B16 cells treated for 24 h with corilagin over 16 mg/L showed typical characteristics of cell injury by morphological observation;B16 cells treated for 24 h with corilagin no more than 16 mg/L showed typical characteristics of cell apoptosis by Hoechst33258 staining.

Key words: additive of cosmetics, corilagin, B16 melanoma cell, tyrosinase

中图分类号: 

  • TQ658