白芨多糖的分子量测定及其吸湿保湿性评价

doi:10.13218/j.cnki.csdc.2015.02.008

摘要/Abstract

摘要： 通过水提醇沉法制取白芨粗多糖,采用Sephadex G-200和Sephadex G-25凝胶柱层析对粗多糖进行纯化,通过红外光谱、高效液相色谱联用多角度激光散射(HPSEC-MALLS)法对纯化后多糖进行结构分析和分子量测定。考察了白芨多糖的吸湿保湿性能,并将其添加到化妆品面霜配方中进行皮肤水分测试。结果表明:红外图谱显示白芨多糖是吡喃型多糖,且含有甘露糖;白芨多糖的重均分子量M_w=9.545×10⁴ g/mol,数均分子量M_n=7.297×10⁴ g/mol;该分子量下的白芨多糖在相对湿度(RH)为43%时最大吸湿率为6.0%,保湿率为88.8%,在RH为81%时最大吸湿率为99.0%,保湿率为105.5%;皮肤水分测试结果表明含白芨多糖液的面霜具有良好的保湿效果。

关键词: 化妆品添加剂, 白芨多糖, 分子量, 吸湿性, 保湿性

Abstract: Crude polysaccharide was prepared by hot water extraction from Rhizoma Bletillae followed by settlement in ethanol aqueous solution.The crude polysaccharide was purified with chromatograph columns Sephadex G-200 and Sephadex G-25.Chemical structure and molecular weight of the purified polysaccharide was analyzed and detected by FTIR analysis and molecular weight test using High Performance Size Exclusion Chromatography Multi-angle Laser Light Scattering method.Hygroscopicity and moisturizing performance of the polysaccharide product were studied.Skin moisturizing test was evaluated by adding the polysaccharide product to cosmetic cream formulations.FTIR showed that the polysaccharide product belongs to pyranose type and contains mannose.M_w and M_n of the polysaccharide product were measured as 9.545×10⁴ g/mol and 7.297×10⁴ g/mol respectively.Under atmospheric condition of RH 43%,maximum hygroscopicity of the polysaccharide product achieves 6.0%,and its moisturizing ratio achieves 88.8%.Under atmospheric condition of RH 81%,maximum hygroscopicity of the polysaccharide product reaches 99.0%,and its moisturizing ratio reaches 105.5%.The results of skin moisture test showed that the polysaccharide product has good moisturizing effect.

Key words: additives of cosmetics, polysaccharide from Rhizoma Bletillae, molecular weight, hygroscopicity, moisturizing performance

中图分类号:

TQ658

孔令姗, 俞苓, 胡国胜, 崔树梅, 王益莉. 白芨多糖的分子量测定及其吸湿保湿性评价[J]. 日用化学工业, 2015, 45(2): 94-98.

KONG Ling-shan, YU Ling, HU Guo-sheng, CUI Shu-mei, WANG Yi-li. Molecular weight,hygroscopicity and moisturizing performance of polysaccharide from Rhizoma Bletillae[J]. China Surfactant Detergent & Cosmetics, 2015, 45(2): 94-98.

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