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日用化学工业（中英文）

日用化学工业 ›› 2014, Vol. 44 ›› Issue (10): 564-567.doi: 10.13218/j.cnki.csdc.2014.10.126

• 开发与应用 • 上一篇    下一篇

桑叶黄酮抑制酪氨酸酶活性的动力学研究

付建红1, 祁瑞2, 郑静2, 周晨婕2, 陈清西2   

  1. 1.新疆师范大学 生命科学学院 新疆特殊环境物种多样性应用与调控重点实验室,新疆 乌鲁木齐 830054;
    2.厦门大学 生命科学学院,福建 厦门 361005
  • 收稿日期:2014-08-08 出版日期:2014-10-22 发布日期:2019-04-08
  • 作者简介:付建红(1970-),女,河南人,副教授,博士,电话:(0991)3693666,E-mail:fjh_719@163.com。
  • 基金资助:
    新疆维吾尔自治区高校科研计划科学研究重点资助项目(XJEDU2012I30);新疆师范大学校级重点学科招标课题资助项目(XJMB-1)

Study of kinetics with respect to action of flavonoid from mulberry leaves on inhibition of activity of tyrosinase

FU Jian-hong1, QI Rui2, ZHENG Jing2, ZHOU Chen-jie2, CHEN Qing-xi2   

  1. 1.Key Laboratory of Species Diversity Application and Control in Xinjiang,College of Life Science,Xinjiang Normal University, Urumqi,Xinjiang 830054,China;
    2.School of Life Sciences,Xiamen University,Xiamen,Fujian 361005,China
  • Received:2014-08-08 Online:2014-10-22 Published:2019-04-08

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摘要： 采用超声波辅助法,以体积分数为60%的乙醇水溶液从新疆桑叶粉末中提取桑叶黄酮。以L-多巴为底物,采用酶动力学方法研究该提取物对酪氨酸酶的抑制作用。实验结果表明,桑叶黄酮提取物对酪氨酸酶有较强的抑制作用,其IC50为0.2 g·L-1。其对酪氨酸酶的抑制作用表现为可逆效应,抑制类型为混合型抑制,对游离酶的抑制常数(KI)和对酶-底物络合物的抑制常数(KIS)分别为366和873 mg·L-1。DPPH实验显示新疆桑叶黄酮提取物具有一定的抗氧化作用,对DPPH自由基氧化作用相对活性的IC50为25 mg·L-1,与VC效果相当。

关键词: 美白化妆品添加剂, 桑叶黄酮, 酪氨酸酶, 抑制机理

Abstract: Flavonoid was extracted from powder of mulberry leaves planted in Xinjiang with 60% (vol) ethanol aqueous solution under assistance of ultrasonic wave.Adopting enzymatic kinetic method,inhibitory action of the extract on tyrosinase activity was studied using L-dopa as the substrate.Results showed that the flavonoid extract displays rather strong inhibitory action on activity of tyrosinase.Its IC50 was estimated as 0.2 g·L-1.The inhibition mechanism was studied and it was found that the reaction is reversible and type of the inhibition belongs to the mixed type,with the inhibition equilibrium constants for inhibitor binding with free enzyme KI and with enzyme-substrate complex KIS were determined as 366 and 873 mg·L-1 respectively.The DPPH (1,1-diphenyl-2-picrylhydrazyl) radical test results showed that the flavonoid extract has the antioxidant activity to a certain extent.The IC50 is 25 mg·L-1 that is similar to vitamin C.

Key words: whitening cosmetics additive, flavonoid from mulberry leaves, tyrosinase, inhibitory mechanism

中图分类号: 

  • TQ658.5