CLAA@CaCO3纳米颗粒稳定双水相Pickering乳液

doi:10.3969/j.issn.1001-1803.2021.12.004

摘要/Abstract

摘要：

双水相体系在生物物质的分离纯化领域具有较好应用前景,但是水-水界面极低的界面张力和较大的界面厚度导致两亲分子无法在界面吸附,因此难以获得稳定的双水相乳液。以共轭亚油酸（CLA）在CaCO₃纳米颗粒表面吸附,在80 ℃下引发CLA分子进行自交联制备得到CLAA@CaCO₃纳米颗粒,并通过紫外（UV）、红外（FT-IR）和热重（TG）表征手段证实CLA双层吸附在CaCO₃纳米颗粒表面。绘制了由硫酸钠（Na₂SO₄）和聚乙二醇20000（PEG 20000）组成的双水相体系的相图,并以CLAA@CaCO₃纳米颗粒稳定Na₂SO₄/PEG 20000双水相Pickering乳液,结果表明：以质量分数0.1%的CLAA@CaCO₃纳米颗粒即可稳定PEG-in-Na₂SO₄双水相Pickering乳液;在同一条系线上,不同两相质量比的乳液均可保持稳定;体系中两种溶质的浓度都必须大于临界浓度（介于系线TL-3和TL-4之间）才可以得到稳定的双水相Pickering乳液。

关键词: 双水相体系, Pickering乳液, 共轭亚油酸, CaCO₃纳米颗粒, 吸附胶束

Abstract:

Aqueous two-phase system （ATPS） with the advantages of low toxicity, environmental friendliness, and good biocompatibility is often used in the field of separation and purification of biological substances. However, ATPS emulsions （the most common name is water-in-water emulsion） are much less known than oil-in-water or water-in-oil emulsions, since amphiphiles such as surfactants are difficult to adsorb on the water-water interface due to the large thickness of the water-water interface and the corresponding ultralow interfacial tension. Therefore, it is a challenge to develop effective stabilizers for water-in-water emulsions. Colloidal particles are one of the commonly used emulsifiers to stabilize water-in-water emulsion. Herein, CLAA@CaCO₃ nanoparticles （NPs） were prepared via adsorbing conjugated linoleic acid admicelle （CLAA） on the surface of CaCO₃ nanoparticles followed by stabilizing CLA molecules through self-crosslinking of conjugated double bonds in the CLA molecules by thermal polymerization at 80 ℃, which was confirmed by UV, FT-IR and TG analyses. The phase diagram of the ATPS composed of sodium sulfate and polyethyleneglycol 20000 （PEG 20000） was drawn and the binodal curve and tie lines were established. Subsequently, the CLAA@CaCO₃ NPs were used as emulsifiers to stabilize the Pickering emulsions of Na₂SO₄/PEG 20000 ATPS. The results show that the CLAA@CaCO₃ NPs can stabilize the PEG-in-Na₂SO₄ Pickering emulsions of ATPS when the mass fraction of the CLAA@CaCO₃ NPs is 0.1%. Emulsions with different two-phase mass ratios on the same tie line （TL） can remain stable and the droplet size is around 50 μm. Moreover, the PEG-in-Na₂SO₄ Pickering emulsions with two-phase mass ratios on TL-1, TL-2 and TL-3 respectively have good long-term stability. It is found that the concentrations of the two solutes in the system should be greater than a lowest threshold concentration as indicated between TL-3 and TL-4 in order to obtain the stable Pickering emulsions of ATPS.

Key words: aqueous two-phase system, Pickering emulsion, conjugated linoleic acid, CaCO₃ nanoparticle, admicelle

中图分类号:

O648

胡佳雯,樊晔,方云,王红. CLAA@CaCO₃纳米颗粒稳定双水相Pickering乳液[J]. 日用化学工业, 2021, 51(12): 1186-1191.

Hu Jiawen,Fan Ye,Fang Yun,Wang Hong. Aqueous two-phase Pickering emulsions stabilized by the CLAA@CaCO₃ nanoparticles[J]. China Surfactant Detergent & Cosmetics, 2021, 51(12): 1186-1191.

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CLAA@CaCO₃纳米颗粒稳定双水相Pickering乳液

Aqueous two-phase Pickering emulsions stabilized by the CLAA@CaCO₃ nanoparticles

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