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日用化学工业（中英文）

日用化学工业 ›› 2014, Vol. 44 ›› Issue (10): 572-576.doi: 10.13218/j.cnki.csdc.2014.10.128

• 开发与应用 • 上一篇    下一篇

几种常用美白剂协同作用研究

王瑞雪1, 赵珍1, 钟雁2, 李小迪2, 王志勇1   

  1. 1.上海医药工业研究院,上海 200437;
    2.健士星生物技术研发(上海)有限公司,上海 200335
  • 收稿日期:2014-08-27 出版日期:2014-10-22 发布日期:2019-04-08
  • 通讯作者: 王志勇,副研究员,电话:(021)55514600,E-mail:wangzhiyong108@126.com。
  • 作者简介:王瑞雪(1988-),女,山东临沂人,硕士研究生,电话:(021)55514600,E-mail:Ruixue@126.com。

Synergistic effect of several kinds of whitening agent on inhibition of tyrosinase activity

WANG Rui-xue1, ZHAO Zhen1, ZHONG Yan2, LI Xiao-di2, WANG Zhi-yong1   

  1. 1.Shanghai Institute of Pharmaceutical Industry,Shanghai 200437,China;
    2.Healthy-star Biological Technology Research and Development (Shanghai) Co.Ltd,Shanghai 200335,China
  • Received:2014-08-27 Online:2014-10-22 Published:2019-04-08

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摘要： 利用酪氨酸酶无细胞体系、B16细胞模型2种反应体系探讨了3种常用美白成分(光甘草定、烟酰胺和VC乙基醚)联合应用对酪氨酸酶的抑制活性。结果表明:2种反应体系中,光甘草定均有最佳的酪氨酸酶抑制活性;正交实验得出3种美白成分复配后光甘草定对酪氨酸酶抑制活性影响最大;应用无细胞及B16细胞反应体系进一步证实3.125 mg·L-1光甘草定分别与不同质量浓度的烟酰胺及VC乙基醚联合使用后,可提高各复配物对酪氨酸酶的抑制活性,对比几种复配物,其中以光甘草定与烟酰胺的复配效果最佳;协同作用分析结果表明,光甘草定与烟酰胺合用可起到酪氨酸酶活性抑制增效的作用,烟酰胺可协同增强光甘草定的美白功效。

关键词: 美白剂, 光甘草定, 烟酰胺, VC乙基醚, 酪氨酸酶

Abstract: Synergistic action of joint application of glabridin,3-O-ethyl-L-ascorbic acid and nicotinamide on tyrosinase activity was investigated using the tyrosinase cell-free system and B16 melanoma cell model.Results showed that in both systems,glabridin displays the highest tyrosinase inhibition efficacy.Via orthogonal designed experiment,action of glabridin in blends of the three kinds of whitening agent on inhibition of tyrosinase activity is identified as the highest.Further test with tyrosinase cell-free system and B16 melanoma system proved that joint use of glabridin of mass concentration 3.125 mg·L-1 with nicotinamide and 3-O-ethyl-L-ascorbic acid of separate mass concentrations can significantly enhance the inhibitory effect on tyrosinase activity.Comparison of experimental results showed that the blend of glabridin and nicotinamide displays the maximum synergistic inhibition action on tyrosinase activity as well as the best whitening efficacy.

Key words: whitening agent, glabridin, nicotinamide, 3-O-ethyl-L-ascorbic acid, tyrosinase

中图分类号: 

  • TQ658