离子色谱法和高效液相色谱法测定化妆品中的丁卡因及其盐类

doi:10.3969/j.issn.1001-1803.2020.09.012

Abstract

Abstract:

Two analytical methods were developed for the determination of tetracaine and its salts in cosmetics based on ion chromatography （IC） and high-performance liquid chromatography （HPLC）. The two detection methods were compared. The limit of detection （LOD） and quantitation （LOQ） of tetracaine were 10 and 25 mg/kg for the IC method. The average recoveries ranged from 87.6% to 102.7% with the relative standard deviations （RSDs） of 1.1%-4.7%. The LOD and LOQ were 2 and 5 mg/kg for the HPLC method. The average recoveries ranged from 89.5% to 105.6% with the RSDs of 1.4%-5.8%. The suspected positive samples were subjected to further verification by high-performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. The chromatographic retention time of tetracaine and its salts was both within 5 min and the experimental data of the two methods met the performance requirements.

Key words: cosmetics, tetracaine and its salt, ion chromatography, high-performance liquid chromatography, mass spectrometric verification

CLC Number:

TQ658

GUO Xiang-yu,ZHAO Wei-zhe,WANG Feng,XIA De-fu,LI Wen-tao,MA Qiang. Determination of tetracaine and its salts in cosmetics by IC and HPLC[J].China Surfactant Detergent & Cosmetics, 2020, 50(9): 648-652.

Figures/Tables 8

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References 15

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化合物	母离子（m/z）	子离子（m/z）	锥孔电压/V	碰撞能量/eV	相对离子丰度/%	允许相对偏差/%
丁卡因	265.1	176.1	3.0	21	100	±25
丁卡因	265.1	71.9	3.0	35	27	±25

方法	提取方法	色谱柱	保留时间/min	检出限/（mg·kg^-1）	定量限/（mg·kg^-1）	回收率/%	相对标准偏差/%
离子色谱法	水提	Metrosep C6	4.3	10	25	87.6 ~ 102.7	1.1 ~ 4.7
高效液相色谱法	醇提	C₁₈	4.4	2	5	89.5 ~ 105.6	1.4 ~ 5.8

Determination of tetracaine and its salts in cosmetics by IC and HPLC

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