紫茉莉花提取物促进胶原蛋白功能化的功效研究

doi:10.3969/j.issn.1001-1803.2019.08.007

Abstract

Abstract:

Human dermal fibroblasts were cultured in vitro, and enzyme-linked immunosorbent assay（ELISA）combined with cell activity assay was used to determine the secretion of BMP-1, which plays an important role in collagen functionalization. The measurement timepoint and positive control of the assay model were optimized. Based on the standardized assay model, the efficacy of Mirabilis jalapa flower extract on BMP-1 synthesis was evaluated. The results show that when treated with Mirabilis jalapa flower extract at 0.05 g/L, the relative BMP-1 content of the fibroblasts is 150.4%±8.5% compared with the control group（P<0.001）. Therefore, the Mirabilis jalapa flower extract can significantly promote the production of BMP-1 from dermal fibroblasts, and has the great potential in skincare effect of promoting collagen functionalization.

Key words: skin care, procollagen C-proteinase, collagen functionalization, human dermal fibroblast, Mirabilis jalapa

CLC Number:

TQ658

LI Hui,JIANG Dan-dan,DOS SANTOS Morgan,ZHANG Zhang,JIANG Yao-quan. Efficacy evaluation of Mirabilis jalapa flower extract on promoting collagen functionalization[J].China Surfactant Detergent & Cosmetics, 2019, 49(8): 526-530.

Figures/Tables 6

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References 14

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t/h	OD值	CV/%
8	0.19	9.1
12	0.37	5.1
18	0.81	7.9
24	1.17	7.7
30	1.55	9.9

实验批次	VC（50 mmol/L）组	TNF-α（10 μg/L）组
第1批	108.5%±14.0%	129.2%±13.5%^*
第2批	88.1%±9.9%^**	141.8%±10.8%^**
第3批	87.7%±22.3%^*	142.9%±5.2%^**

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Efficacy evaluation of Mirabilis jalapa flower extract on promoting collagen functionalization

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