高效液相色谱-串联质谱法快速测定护肤透明质酸中抗菌药物

doi:10.3969/j.issn.1001-1803.2022.06.015

Abstract

Abstract:

The aim was to establish a rapid method for the determination of antibiotics and nitroimidazoles in skin care hyaluronic acid by high performance liquid chromatography tandem mass spectrometry. Ten reagents for each of the two kinds of antibiotics were selected. After vortex oscillation, ultrasonic extraction, centrifugation and filtration, the mixed solution samples of the two kinds of antibiotics were prepared. They were separated by chromatographic column, ionized by electrospray ionization in positive ion mode and detected by multiple reaction monitoring mode under gradient elution. The results show that the total ions of 10 compounds contained in the solution samples of the two antibiotics have separation reactions at different times and produce corresponding response values, showing a good linear correlation. The correlation coefficient results are 0.997 and above 0.996, respectively. The detection limits and quantitative limits of the two antibiotics are 1.6, 6 mg/kg, 0.9-201 mg/kg and 5-401 mg/kg, respectively. The method can accurately and efficiently complete the rapid determination of antibiotics and nitroimidazoles in skin care hyaluronic acid.

Key words: high performance liquid chromatography-tandem mass spectrometry, rapid determination, skin care hyaluronic acid, antibacterial drugs

CLC Number:

TQ658

Zhang Junfang. Rapid determination of antibacterial agents in skin care hyaluronic acid by high performance liquid chromatography-tandem mass spectrometry[J].China Surfactant Detergent & Cosmetics, 2022, 52(6): 677-682.

Figures/Tables 10

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References 15

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时间/min	φ（A）/%	φ（B）/%
0	80	20
30	70	30
50	30	70
51	80	20
55	80	20

化合物成分	保留时间/min	母离子（m/z）	子离子（m/z）	裂解电压/V	碰撞电压/V
恩诺沙星	4.17	321.1	303.1	55	32
恩诺沙星	4.17	321.1	232.1	55	27
环丙沙星	4.76	332.4	288.2	85	31
环丙沙星	4.76	332.4	245.1	85	26
诺氟沙星	4.56	320.2	276.1	55	26
诺氟沙星	4.56	320.2	233.2	55	36
氧氟沙星	4.57	362.4	318.3	70	28
氧氟沙星	4.57	362.4	261.1	70	38
双氟沙星	6.33	400.4	356.3	85	35
双氟沙星	6.33	400.4	299.2	85	29
司帕沙星	6.32	393.6	349.3	70	30
司帕沙星	6.32	393.6	292.3	70	36
加替沙星	4.36	376.4	332.4	60	25
加替沙星	4.36	376.4	281.2	60	37
丹诺沙星	4.94	358.2	340.3	55	28
丹诺沙星	4.94	358.2	283.3	55	35
氟罗沙星	4.57	362.4	318.3	80	28
氟罗沙星	4.57	362.4	261.1	80	39
麻保沙星	4.25	363.2	320.3	60	24
麻保沙星	4.25	363.2	345.3	60	30

化合物成分	保留时间/min	母离子（m/z）	子离子（m/z）	裂解电压/V	碰撞电压/V
4-硝基咪唑	3.21	114.3	68.3	30	16
4-硝基咪唑	3.21	114.3	84.1	30	13
羟基甲硝唑	4.47	188.3	123.3	25	16
羟基甲硝唑	4.47	188.3	126.3	25	15
羟基异丙硝唑	10.61	186.3	168.3	25	16
羟基异丙硝唑	10.61	186.3	122.3	25	21
异丙硝唑	13.23	170.3	124.3	35	18
异丙硝唑	13.23	170.3	109.3	35	25
甲硝唑	5.51	172.3	128.3	25	16
甲硝唑	5.51	172.3	82.2	25	21
奥硝唑	11.07	220.3	128.3	30	16
奥硝唑	11.07	220.3	82.2	30	26
洛硝哒唑	6.92	201.3	140.3	20	13
洛硝哒唑	6.92	201.3	55.3	20	21
替硝唑	9.39	248.3	121.3	30	16
替硝唑	9.39	248.3	128.3	30	21
苯硝咪唑	7..66	164.3	118.3	35	19
苯硝咪唑	7..66	164.3	91.2	35	31
氯甲硝咪唑	8.93	162.3	116.2	30	18
氯甲硝咪唑	8.93	162.3	145.2	30	16

化合物成分	相关系数	检出限/ （mg/kg）	定量限/ （mg/kg）
恩诺沙星	0.999 2	1.6	6
环丙沙星	0.999 8
诺氟沙星	0.999 8
氧氟沙星	0.999 7
双氟沙星	0.999 8
司帕沙星	0.999 7
加替沙星	0.999 4
丹诺沙星	0.999 6
氟罗沙星	0.999 4
麻保沙星	0.997 2

化合物成分	相关系数	检出限/ （mg/kg）	定量限/ （mg/kg）
4-硝基咪唑	0.999 6	41	201
羟基甲硝唑	0.999 7	5	21
羟基异丙硝唑	0.998 9	201	401
异丙硝唑	0.998 6	0.9	5
甲硝唑	0.999 5	9	21
奥硝唑	0.998 4	3	5
替硝唑	0.998 8	0.9	5
苯硝咪唑	0.999 1	9	21
洛硝哒唑	0.996 4	9	21
氯甲硝咪唑	0.998 7	21	41

Rapid determination of antibacterial agents in skin care hyaluronic acid by high performance liquid chromatography-tandem mass spectrometry

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