白果内酯和银杏内酯B对过氧化氢诱导人角质形成细胞氧化损伤的保护作用

doi:10.3969/j.issn.1001-1803.2019.08.008

Abstract

Abstract:

Different concentrations of bilobalide and ginkgolides B were added to H₂O₂-stimulated HaCaT model. Cell viability was determined by CCK-8 method, superoxide dismutase（SOD）activity was determined by WST staining method, glutathione（GSH）activity was measured by DTNB, malondialdehyde（MDA）content was detected by lipid oxidation kit. The content of ROS was investigated via DCFH-DA method. And ELISA assays were adopted to investigate the production of TNF-α, IL-1β, and IL-6. To better understand the mechanism of the efficacy of bilobalide and ginkgolides B, TCM database（IUPHAR/BPS Guide to Pharmacology and String）was used to predict common targets of drugs. The results show that bilobalide and ginkgolides B can increase the SOD and GSH-Px activity, decrease MDA and ROS content induced by H₂O₂, and reduce the production of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 in H₂O₂-induced HaCaT cells. The drug discovery database shows that the anti-inflammatory effect of bilobalide and ginkgolides B has the characteristics as multi-molecular, multi-target and multi-pathway.

Key words: bilobalide, ginkgolides B, inflammatory factor, ROS, in vitro test

CLC Number:

TQ658

XIONG Li-dan,TANG Jie,LI Li. Protective effects of bilobalide and ginkgolides B on oxidative damage in human keratinocytes induced by H₂O₂[J].China Surfactant Detergent & Cosmetics, 2019, 49(8): 531-536.

Figures/Tables 5

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Tab. 1

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References 13

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分组	SOD活力/（U·mL^-1）	GSH-Px活力/（U·g^-1）	MDA含量/（μmol·L^-1）
空白对照组	32.18±2.44^##	202.44±10.41^##	2.87±0.34^##
H₂O₂模型组	20.31±1.80^**	126.57±6.89^**	14.55±2.33^**
BB 10 μmol/L + H₂O₂模型组	26.16±3.72^**##	132.44±8.06^**#	8.89±1.66^**#
BB 25 μmol/L + H₂O₂模型组	27.90±2.58^**##	140.67±9.40^**#	8.43±1.67^**#
BB 50 μmol/L + H₂O₂模型组	22.66±1.46^**	139.46±5.37^**#	8.22±0.93^**#
GB 10 μmol/L + H₂O₂模型组	23.03±2.36^**#	142.56±6.12^**#	10.31±2.40^**#
GB 25 μmol/L + H₂O₂模型组	24.17±1.73^**##	146.48±8.33^**##	8.31±1.45^**#
GB 50 μmol/L + H₂O₂模型组	26.06±2.78^**##	151.59±9.45^**##	7.12±0.91^**##

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Protective effects of bilobalide and ginkgolides B on oxidative damage in human keratinocytes induced by H₂O₂

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