Abstract: Tranexamic acid,nicotinamide,potassium 4-methoxysalicylate and Glycyrrhiza glabra (licorice) root extract were used as the main whitening active ingredients to prepare a human-skin whitening cream.The prepared human-skin whitening cream is uniform,no granulous feeling,delicate and smooth,with good consistency and stability.The whitening effect was evaluated by 3D melanin skin model in vitro test and volunteer's human-skin test.The results of 3D melanin skin model in vitro test show that the prepared human-skin whitening cream displays significant inhibitory effect on the synthesis or transfer of melanin,which indicates that it has excellent whitening effect in vitro.Human-skin test results show that the skin melanin content (MI value) of the volunteer's skin is significantly reduced,and their skin whiteness (L^* value) and brightness (ITA° value) are increased after using the prepared human-skin whitening cream for 4 weeks,indicating that the prepared human-skin whitening cream displays a good effect.

Key words: whitening cosmetic, 3D melanin skin model, in vitro test, human-skin test