茶皂素的纯化及抑制酪氨酸酶活性的研究

doi:10.13218/j.cnki.csdc.2017.06.003

Abstract

Abstract: Purification of tea saponin as well as inhibitory effect and inhibiting mechanism for catalytic synthesis of DOPA quinone from L-DOPA by potato tyrosinase in vitro was examined.The tea saponin product that purchased from market,was treated with macroporous resin to get the preliminarily refined tea saponin product (TS).Then the TS were further purified by chromatographic method and two kinds of purified tea saponin product were produced as TS1 and TS2.Results show that at mass concentration of 100 g/L,the inhibition rate of the market product,the TS,TS1 and TS2 achieves 51.29%,90.06%,94.13% and 82.47% respectively.IC₅₀ of TS and TS1 achieves 48 and 40 g/L respectively.The inhibition kinetics analyzed by Lineweaver-Burk plots show that the action of TS and TS1 express as reversible and competitive inhibition for conversion of L-DOPA.The inhibition constants for the TS and TS1 achieves 137 and 101 g/L respectively.

Key words: whitening cosmetic additive, tea saponin, tyrosinase, inhibition

CLC Number:

TQ658

SUN Jin-yi, CAI Chun-hui, LIANG Rong, YANG Cheng. Purification of tea saponin and its inhibition on tyrosinase activity[J].China Surfactant Detergent & Cosmetics, 2017, 47(6): 312-316.

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Purification of tea saponin and its inhibition on tyrosinase activity

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