海藻酸钠对酪氨酸酶的抑制作用

doi:10.3969/j.issn.1001-1803.2019.06.007

Abstract

Abstract:

Enzyme inhibition kinetics experiments were carried out in vitro and in melanocytes to study the inhibitory effect of sodium alginate on the activity of tyrosinase and melanin deposition. The inhibition mechanism of sodium alginate on tyrosinase was analyzed by fluorescence spectroscopy, and the toxicity of sodium alginate on melanocyte was also investigated. The results show that within the concentration of 64 mmol/L, the cell viability was above 90%, indicating good biocompatibility. Sodium alginate had a certain inhibitory effect on tyrosinase activity and melanin production, which was in a concentration-dependent manner below the concentration of 20 mmol/L. The endogenous fluorescence of tyrosinase was partly quenched after interacting with sodium alginate. The redshift of the maximum emission wavelength appearing in the fluorescence spectrum of tyrosinase indicated that the interaction between sodium alginate and tyrosinase changed the hydrophobic environment of its amino acid residues.

Key words: whitening cosmetic additive, sodium alginate, tyrosinase, inhibitor, inhibitioon mechanism

CLC Number:

TQ658

ZHENG Xi,CHEN Zhi-duo,ZHANG De-meng,QIN Yi-min,YI Cai-xia,HUANG Xiao. Inhibitory effect of sodium alginate on the activity of tyrosinase[J].China Surfactant Detergent & Cosmetics, 2019, 49(6): 388-392.

Figures/Tables 5

References 23

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Inhibitory effect of sodium alginate on the activity of tyrosinase

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