Abstract:

In order to obtain the active components of Salvia plebeia R. Br. for antioxidant and anti-inflammatory activity, the macroporous resin elution process was selected based on the content of total flavonoids and phenolic acid. The antioxidant activity of Salvia plebeia R. Br. extracts （SE） was detected by DPPH free radical scavenging method and ABTS total antioxidant capacity method. UVB-induced human skin immortalized keratinocytes and mouse dermatitis model constructed with 2, 4-dinitrofluorobenzene （DNFB） solution were used to test the anti-inflammatory effect of SE. The total flavonoids content of SE is 36.42%±0.45% and the total phenolic acid content is 21.32%±0.82%. The half maximal inhibitory mass concentrations of DPPH and ABTS are （49.09±0.10） μg/mL and （84.54±0.99） μg/mL, respectively. In vitro, SE can inhibit the secretion of IL-6, IL-1α and TNF-α in human immortalized keratinocytes. In vivo, SE can effectively relieve ear skin redness and swelling, and reduce the number of inflammatory cells and mast cells degranulation, as well as has significant inhibitory effects on the secretion of IFN-γ, IL-4, IL-5, IgE, and IL-17 in mice. In addition, SE can reduce the thymus index and spleen index in mice. This study proves that SE has excellent antioxidant and anti-inflammatory effects, which can provide a basis for high value utilization of SE in the field of daily chemicals.

Key words: Salvia plebeia R. Br. extracts, antioxidant, mice, anti-inflammation