Abstract:

A high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） method has been established for the simultaneous determination of 10 banned stines drugs in 3 different matrixes of cosmetics. The sample was ultrasonically extracted with 0.1% formic acid-acetonitrile and performed on an Agilent Eclipse plus C₈ column （2.1 mm×100 mm, 1.8 μm） with gradient elution using methanol and 5 mmol/L ammonium formate-0.1% formic acid aqueous solution as the mobile phase. The determination was performed by MS/MS in multiple reaction detection （MRM） mode, and the external standard method was used for quantification. Betahistine dihydrochlorride, mizolastine, epinastine hydrochloride, and emedastine difumarate have good linearity in the range of 1-16 μg/L, while the other six stines have good linearity in the range of 0.1-1.6 μg/L, with the correlation coefficient （r²）>0.99. The limits of detection （LODs） and the limits of quantification （LOQs） are in the range of 0.003-0.12 μg/L and 0.011-0.39 μg/L, respectively. At three different levels of addition, the recoveries of water aqua, lotion and cream samples are 88.0%-107.8%, 87.1%-106.5%, and 87.1%-107.7%, respectively. And the relative standard deviation （RSD） is less than 10%. The method is accurate, high-efficiency, simple pre-treatment, and high recovery rate. It is suitable for the simultaneous determination of 10 stines in cosmetics.

Key words: high performance liquid chromatography-tandem mass spectrometry, cosmetic, prohibited drug, antihistamines, stines