虎乳芝提取物的护肤功效及安全性评价研究

doi:10.3969/j.issn.2097-2806.2026.06.008

Abstract

Abstract:

This study focused on the aqueous extract of Lignosus rhinocerus, investigating its anti-aging, repair, anti-inflammatory, and oil-control effects through antioxidant, anti-glycation, cell proliferation, and related gene expression tests. The anti-inflammatory effect was evaluated by the nitric oxide （NO） inhibition assay, and the oil-control potential was assessed by quantifying the expression of genes related to sebum secretion, as well as intracellular lipids, and lipid droplets. Skin safety was evaluated through skin irritation tests. The results show that the Lignosus rhinocerus extract effectively scavenges DPPH free radicals, significantly reduces the production of glycation products, and promotes the expression of anti-aging-related genes （P<0.05）, demonstrating antioxidant, anti-glycation, and anti-wrinkle effects. Furthermore, the extract also promotes keratinocyte proliferation and increases hyaluronic acid synthase gene expression, indicating repair effects. Moreover, it significantly inhibits the production of nitric oxide induced by lipopolysaccharide （P<0.05）, showing anti-inflammatory effects. It significantly suppresses the expression of genes related to sebum secretion, and limits linoleic acid-induced sebum synthesis and lipid droplet formation （P<0.05）, indicating potential oil-control ability. The results of the human skin model irritation test further confirm the high skin safety of the extract. This study demonstrates that the Lignosus rhinocerus extract has anti-aging, repair, anti-inflammatory, and oil-control activities, combining efficacy with safety, which makes it a highly valuable candidate for cosmetic ingredient development.

Key words: Lignosus rhinocerus extract, anti-aging, anti-glycation, repair, soothing, oil-control, safety assessment

CLC Number:

TQ658

Yiwen Chen, Yali Zheng, Xin Li, Jiancong Huang, Rongkang Kuang, Xiaobao Xie. Study on the skincare effects and safety evaluation of the Lignosus rhinocerus extract[J].China Surfactant Detergent & Cosmetics, 2026, 56(6): 764-775.

Figures/Tables 14

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References 31

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Primer	Forward	Reverse
COL1	TGGTGGTTATGACTTTGGTTACGAT	TGTGCGAGCTGGGTTCTTTCTA
COL3A1	GGAGCTGGCTACTTCTCGC	GGGAACATCCTCCTTCAACAG
LAMA3	AACCAAAAAGTGCTCGGAAGAC	TCAGTAGGTGGTAAGCCCAAATC
LAMC2	TACAGAGCTGGAAGGCAGGATG	GTTCTCTTGGCTCCTCACCTTG
COL4A2	GTACTGCAACCCTGGTGATGTCTGC	GAATATCCGATCCACAAACTCCGCC
LC3A	CGGACATCTACGAGCAGGA	AGAGGCAGCTCAGTTCAGGA
ATG5	AACTGAAAGGGAAGCAGAACCA	CCATTTCAGTGGTGTGCCTTC
BECLIN1	AGCTGCCGTTATACTGTTCTG	ACTGCCTCCTGTGTCTTCAATCTT
HAS2	TTCTTTATGTGACTCATCTGTCTCACCGG	ATTGTTGGCTACCAGTTTATCCAAACG

成分	含量/ （mg/g）
碳水化合物	817.6±1.5
蛋白质	115.0±0.1
总酚	2.9±0.1

No.	保留时间/min	分子式	分子量	面积/%	名称
1	5.628	C₂H₆O	46.04	2.76	Dimethyl ether
2	7.304	C₅H₁₂O₂	104.08	0.09	（2R, 4R） - （-） -Pentanediol
3	21.455	C₁₉H₃₀O₃	306.22	4.21	Pentanoic acid, 5-hydroxy-, 2, 4-di-t-butylphenyl esters
4	24.155	C₁₂H₈O₅	232.04	2.35	1, 4-Naphthoquinone, 6-acetyl-2, 5-dihydroxy-
5	30.231	C₁₃H₂₈	184.22	1.07	Decane, 3, 3, 8-trimethyl-
6	30.304	C₁₇H₂₄O₃	276.17	10.15	7, 9-Di-tert-butyl-1-oxaspiro （4, 5） deca-6, 9-diene-2, 8-dione
7	31.176	C₁₇H₂₄O₄	292.17	1.66	Phthalic acid, 2-ethylbutyl propyl ester
8	31.642	C₁₈H₃₆O₂	284.27	18.39	Hexadecanoic acid, ethyl ester
9	34.023	C₄H₇NO	85.05	0.12	Propane, 1-isocyanato-
10	34.802	C₂₀H₃₈O₂	310.29	10.01	（E） -9-Octadecenoic acid ethyl ester
11	35.255	C₂₀H₄₀O₂	312.3	6.80	Octadecanoic acid, ethyl ester
12	39.141	C₂₃H₃₂O₂	340.24	10.60	Phenol, 2, 2'-methylenebis[6- （1, 1-dimethylethyl） -4-methyl-
13	41.085	C₇H₁₂O₂	128.08	2.43	2, 4-Heptanedione
14	44.289	C₇H₇Cl₂NO	190.99	4.75	Clopidol
15	48.392	C₁₁H₇N₅	209.07	0.34	1, 3, 6, 9b-Tetraazaphenalene-4-carbonitrile, 2-methyl-
16	51.535	C₁₀H₁₁NO₃	193.07	2.07	Terephthalaldehydic acid, methyl ester, p- （O-methyloxime）
17	52.248	C₂₃H₂₃NO₂	345.17	8.35	Benzamide, 2-methoxy-N-benzyl-N-phenethyl-
18	52.867	C₁₈H₂₆O₄	306.18	0.83	Phthalic acid, ethyl 4-octyl ester
19	53.189	C₂₄H₂₂	310.17	2.28	1, 2, 3-Triphenyl-3-isopropyl-cyclopropene
20	53.35	C₁₀H₁₁NO₃	193.07	4.17	2, 4, 6-Trimethoxybenzonitrile
21	56.463	C₁₇H₁₄O₄	282.09	2.49	4H-1-Benzopyran-4-one, 6, 7-dimethoxy-3-phenyl-
22	56.794	C₂₀H₂₂O₅	342.15	1.35	Terephthalic acid, isobutyl 2-phenoxyethyl ester
23	57.778	C₁₄H₂₇N	209.21	0.86	（5R, 8R, 8aS） -5, 8-Dipropyloctahydroindolizine
24	57.867	C₂₀H₂₃NO₄	341.16	1.86	2- （3, 5-Dimethoxyphenyl） -5, 6-dimethoxy-1, 3-dimethyl-1H-indole-

Study on the skincare effects and safety evaluation of the Lignosus rhinocerus extract

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