Abstract:

A method for the determination of 8-hydroxyquinoline in different types of cosmetics by high performance liquid chromatography（HPLC）was established. 8-Hydroxyquinoline was dissolved in methanol, and then separated by SHISEIDO C₁₈（4.6 mm×250 mm, 5 μm）column using 0.1% formic acid and methanol or 0.1% phosphoric acid and methanol as mobile phase with isocratic elution, with the detection wavelengths at 254 nm. Compared with the standard method, the mobile phase in this study used formic acid or phosphoric acid instead of sodium decane-1-sulfonate to avoid the peak of hydroxyquinoline salt in the chromatogram of the reference substance. The calibration curves show good linear relationship in the range of injection volume and the correlation coefficients are above 0.999. The average recoveries spiked at 80%, 100%, 120% are in the range of 96.9%-112.9% with RSD of 0.20%-2.55%（n=9）. The established method is simple, reliable, and has high sensitivity and reproducibility. The method can be used for determing the content of 8-hydroxyquinoline in cosmetics.

Key words: cosmetics, high performance liquid chromatography, 8-hydroxyquinoline