Abstract: A method based on ultra performance liquid chromatography-diode array detector (UPLC-DAD) for simultaneous determination of ozagrel,niacinamide,ferulic acid,phenol,salicylic acid,phenylethyl resorcinol and octanoylsalicylic acid in whitening cosmetics was established.These seven compounds were extracted by 50% methanol aqueous solution under assistance of ultrasonic wave for 20 min.ACQUITY UPLC HSS T₃ column (2.1 mm×50 mm,1.8 μm) was used with mobile phase of sodium 1-decanesulfonate with concentration of 5 mmol/L (containing 0.1% phosphoric acid) for conducting gradient elution.The detection wavelength is 220 and 277 nm,and the column temperature is 30 ℃.The linearity ranges in 0.25~10 mg/L for ozagrel,0.5~20 mg/L for octanoylsalicylic acid,2.0~80 mg/L for salicylic acid as well as 1.0~40 mg/L for niacinamide,ferulic acid,phenol and phenylethyl resorcinol.The intra and inter day precision of retention time and peak area are also good.The average recovery rates are from 91.9% to 108.2%,and the relative standard deviations (RSD) are from 0.1% to 2.6%.The method is convenient,accurate,reliable and suitable for the determination of seven compounds above mentioned at the same time.

Key words: cosmetics, ultra performance liquid chromatography, whitening ingredients