凝胶渗透色谱-液相色谱-串联质谱法同时测定化妆品中15种糖皮质激素和性激素

doi:10.13218/j.cnki.csdc.2014.05.066

Abstract

Abstract: A method for the simultaneous determination of residue of 15 kinds of glucocorticoid and sex hormone in cosmetics by gel permeation chromatography-liquid chromatography-tandem mass spectrometry (GPC-LC-MS/MS) was established.The sample was extracted with acetonitrile under ultrasonic assistance,and the extract was purified with GPC column of cyclohexane-ethyl acetate.Then the contents in the purified extract were separated by a C₁₈ column with acetonitrile-0.1% formic acid solution as the mobile phase,and then detected by LC-MS/MS.Testing results showed that good linearity was obtained with correlation coefficients higher than 0.995.The detection limit ranges within 0.1-4.0 mg·kg^-1.The spiked recovery ranges within 70.0%-104.0%,and the RSD ranges within 5.3%-15.5%.

Key words: cosmetics, glucocorticoid, sex hormone, GPC, LC-MS/MS

CLC Number:

TQ658

CUI Han, SHEN Bao-zhen, CHEN Xi, CAO Wen-jun, LIU Meng-yao, HUANG Da-liang. Simultaneous determination of 15 kinds of glucocorticoid and sex hormone in cosmetics by GPC-LC-MS/MS[J].China Surfactant Detergent & Cosmetics, 2014, 44(5): 295-298.

References

[1] 卢杰,冯晓梅,隋晓,等.亚临界1,1,1,2-四氟乙烷萃取-气相色谱-质谱法测定鱼肉中6种性激素残留[J].分析化学,2013,41(10):1487-1492.
[2] 中华人民共和国卫生部.GB/T 7917.3—1987 化妆品卫生规范[S].北京:中国卫生出版社,2007.
[3] 中华人民共和国国家质量监督检验检疫总局.GB/T 24800.2—2009 化妆品中四十一种糖皮质激素的测定液相色谱/串联质谱法和薄层层析法[S].北京:中国标准出版社,2009.
[4] WANG L,CAI Y,HE B,et al.Determination of estrogens in water by HPLC-UV using cloud point extraction [J].Talanta,2006,70:47-51.
[5] QUINTANA J B,CARPINTEIRO J,RODRIGUEZ I,et al.Determination of natural and synthetic estrogens in water by gas chromatography with mass spectrometric detection [J].Journal of Chromatography A,2004,1024:177-185.
[6] SEO J J,KIM H Y,CHUNG B C,et al.Simultaneous determination of anabolic steroids and synthetic hormones in meat by freezing-lipid filtration,solid-phase extraction and gas chromatography-mass spectrometry [J].Journal of Chromatography A,2005,1067:303-309.
[7] MAZZARINO M.Effect of the systemic versus inhalatory administration of synthetic glucocorticoids on the urinary steroid profile as studied by gas chromatography-mass spectrometry [J].Analytica Chimica Acta,2006,559:30-36.
[8] LIANG C X,BING S,RONG Z,et al.Simultaneous determination of twelve glucocorticoid residues in milk by ultra performance liquid chromatography-electrospray tandem mass spectrometry [J].Chinese Journal of Chromatography,2006,24(24):213-217.
[9] OLIVIA V D H,FREDERIC D,CHRIS E,et al.Carlos Van Peteghem detection of synthetic glucocorticoid residues in cattle tissue and hair samples after a single dose administration using LC-MS/MS [J].Journal of Chromatography B,2005,817:215-223.

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Simultaneous determination of 15 kinds of glucocorticoid and sex hormone in cosmetics by GPC-LC-MS/MS

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