重组Ⅲ型胶原蛋白对皮肤光老化的改善作用研究

doi:10.3969/j.issn.2097-2806.2025.11.011

Abstract

Abstract:

The aim of this study was to investigate the mechanism of recombinant human collagen Ⅲ （RHC-Ⅲ） on anti-skin photoaging. The blank control group, model control group, positive control group and sample groups were set up in the experiment. Primary human skin cells and ex vivo human skin tissue model were used to detect the changes of photoaging markers in dermal-epidermal junction （DEJ） and dermis by crystal violet staining, enzyme-linked immunosorbent assay （ELISA） and immunofluorescence. The results of the cell experiments show that compared with the blank control group, RHC-Ⅲ significantly increases the adhesion ability of normal human dermal fibroblasts （NHDFs）. Compared with the model control group, RHC-Ⅲ significantly decreases the level of ROS, MMPs secretion and increases the activity of CAT. In ex vivo human skin tissue model experiments, compared with the model control group, RHC-Ⅲ significantly increases the contents of COL-Ⅳ, COL-Ⅶ, COL-ⅩⅦ, integrin α6 subunit, COL-Ⅰ, COL-Ⅲ and elastin. The results indicate that the mechanism of RHC-Ⅲ improving skin photoaging is related to promoting NHDFs adhesion, reducing oxidative stress and the degradation of extracellular matrix, improving the expression of DEJ structure-related proteins, and increasing the synthesis of key extracellular matrix proteins in dermis.

Key words: recombinant human collagen Ⅲ, photoaging, ex vivo human skin model, oxidative stress, dermal-epidermal junction

CLC Number:

TQ658

Fei Liu, Qian Wang, Hui Liu, Suzhen Yang, Tingting Han, Tao Wu. Study on the effect of recombinant human collagen Ⅲ on anti-skin photoaging[J].China Surfactant Detergent & Cosmetics, 2025, 55(11): 1452-1559.

Figures/Tables 5

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References 27

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ρ（RHC-Ⅲ）/ （mg/mL）	Cell viability/%
ρ（RHC-Ⅲ）/ （mg/mL）	NHEKs	NHDFs
0	100.00±1.99	100.00±3.89
0.08	96.05±4.47	101.30±4.92
0.16	96.36±6.26	101.04±2.61
0.31	95.60±1.74	99.06±3.77
0.63	95.92±3.16	99.69±3.34
1.25	94.26±4.34	99.69±4.86
2.50	93.49±3.47	97.29±3.38
5.00	90.88±2.55	96.30±4.02
10.00	72.19±3.18	96.04±6.32

Study on the effect of recombinant human collagen Ⅲ on anti-skin photoaging

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