高效液相色谱法与离子色谱法测定化妆品原料乙酰基六肽-8中醋酸和三氟乙酸的比较研究

doi:10.3969/j.issn.2097-2806.2025.08.016

Abstract

Abstract:

The study aims to investigate the consistency of analysis results of acetic acid and trifluoroacetic acid in cosmetic raw material acetyl hexapeptide-8 by high performance liquid chromatography （HPLC） and ion chromatography （IC）. The methods of determination of acetic acid and trifluoroacetic acid by HPLC and IC were improved and optimized. The specificity, linear range, detection limit, quantitation limit, recovery, precision, repeatability and stability were compared. The results of 15 batches acid and trifluoroacetic acid in acetyl hexapeptide-8 raw materials were analyzed statistically. The results show that both two methods have good specificity, and the linear range of IC method is wider than that of HPLC method, with lower detection and quantification limits. However, the precision of HPLC method is better. Both two methods can be used for qualitative and quantitative analysis of acetic acid and trifluoroacetic acid in acetyl hexapeptide-8. There is no statistical difference between the testing results of HPLC and IC to determine acetic acid in acetyl hexapeptide-8 （|t|=0.200, P>0.05）. Because two methods have their own advantages, the appropriate analysis method can be selected according to the actual situation.

Key words: acetic acid, trifluoroacetic acid, high performance liquid chromatography, ion chromatography, acetyl hexapeptide-8

CLC Number:

TQ658

Cen Chen, Xiaomin Dai, Linqi Yan, Qiaoyuan Cheng. Comparison of high performance liquid chromatography and ion chromatography for the determination of acetic acid and trifluoroacetic acid in cosmetic raw material acetyl hexapeptide-8[J].China Surfactant Detergent & Cosmetics, 2025, 55(8): 1078-1084.

Figures/Tables 6

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References 21

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化合物	方法	线性范围/（mg/L）	回归方程	相关系数r	LOD/（mg/L）	LOQ/（mg/L）
醋酸	HPLC	5~200	Y=368.611 8X+1.568 6	1.000 0	0.51	2.02
醋酸	IC	1~200	Y=0.019 9X+0.046 4	0.999 8	0.13	0.46
三氟乙酸	HPLC	5~200	Y=213.443 7X-160.548 5	0.999 9	1.36	4.69
三氟乙酸	IC	1~200	Y=0.049 9X-0.044 4	0.999 9	0.17	0.59

化合物	加标质量浓度水平/（mg/L）	平均加标回收率/%		RSD/%
化合物	加标质量浓度水平/（mg/L）	HPLC	IC	HPLC	IC
醋酸	10	102.0	99.0	0.61	1.02
	100	99.5	103.3	0.30	0.59
	160	99.0	104.2	0.31	0.48
三氟乙酸	10	101.0	99.6	1.25	0.39
	100	100.3	98.9	0.65	0.22
	160	99.5	100.7	0.60	1.64

化合物	重复性含量/%		重复性RSD/%		稳定性RSD/%
化合物	HPLC	IC	HPLC	IC	HPLC	IC
醋酸	0.52	0.51	0.77	0.97	0.92	0.79
三氟乙酸	2.49	2.51	0.21	0.22	1.16	0.42

生产企业	批号	醋酸含量/%		平均值（（w₁+w₂） /2） /%	相对偏差/%	三氟乙酸含量/%
生产企业	批号	HPLC （w₁）	IC （w₂）	平均值（（w₁+w₂） /2） /%	相对偏差/%	HPLC	IC
A	240401	0.63	0.62	0.625	1.6	ND	ND
	240402	0.52	0.51	0.515	1.9	ND	ND
	240403	0.64	0.64	0.64	0	ND	ND
B	P2024022301	3.89	3.91	3.90	0.5	ND	ND
	P2024022611	4.14	4.14	4.14	0	ND	ND
	P2024022701	3.99	3.98	3.985	0.3	ND	ND
C	M20240303	ND	ND	—	—	ND	ND
	M20240415	ND	ND	—	—	2.49	2.51
	M20240416	ND	ND	—	—	2.73	2.72
D	20231101	1.93	1.92	1.925	0.5	ND	ND
	20231201	1.80	1.78	1.79	1.1	ND	ND
	20240101	1.92	1.93	1.925	0.5	ND	ND
E	C429D150G0-1	3.31	3.33	3.32	0.6	ND	ND
	C429D150G0-2	2.97	2.97	2.97	0	ND	ND
	C429D150G0-3	3.13	3.15	3.14	0.6	ND	ND

Comparison of high performance liquid chromatography and ion chromatography for the determination of acetic acid and trifluoroacetic acid in cosmetic raw material acetyl hexapeptide-8

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