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日用化学工业（中英文）

日用化学工业 ›› 2014, Vol. 44 ›› Issue (9): 501-503.doi: 10.13218/j.cnki.csdc.2014.09.112

• 开发与应用 • 上一篇    下一篇

N-乙酰-L-半胱氨酸抗 H2O2诱导人体皮肤成纤维细胞损伤的研究

王风楼1, 朱丽平2, 马霞1   

  1. 1.上海应用技术学院 香料香精技术与工程学院,上海 201418;
    2.莱博实业(上海)股份有限公司,上海 200233
  • 收稿日期:2014-03-11 出版日期:2014-09-22 发布日期:2019-04-08
  • 通讯作者: 马 霞,教授,博士,电话:(021)60873210,E-mail:maxia0126@126.com。
  • 作者简介:王风楼(1988-),女,河北人,硕士研究生,电话:18817801132,E-mail:wfl3164@163.com。

Action of N-acetyl-L-cystein for resisting human skin fibroblast damage induced by H2O2

WANG Feng-lou1, ZHU Li-ping2, MA Xia1   

  1. 1.School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China;
    2.LB Nature (Shanghai) Co.,Ltd,Shanghai 200233,China
  • Received:2014-03-11 Online:2014-09-22 Published:2019-04-08

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摘要： 研究了N-乙酰-L-半胱氨酸(NAC)对H2O2刺激的人体皮肤成纤维细胞(HSF)的抗细胞损伤作用。细胞存活率和细胞阳性率实验结果表明:NAC在低质量浓度(50～100 mg·L-1)时对HSF没有明显的细胞毒性;在质量浓度为200～800 mg·L-1时可促进细胞增殖;而在质量浓度较高(1 000 mg·L-1)时则明显抑制HSF生长。空白组、H2O2组、H2O2 (500 μmol·L-1)+NAC (100 mg·L-1)组和H2O2 (500 μmol·L-1)+NAC (200 mg·L-1)组细胞阳性率分别为39.90%,69.57%,45.29%和36.60%,NAC可明显降低β-半乳糖苷酶染色阳性率,抵抗由H2O2引起的细胞损伤。

关键词: 化妆品添加剂, N-乙酰-L-半胱氨酸, 人体皮肤成纤维细胞, 毒性, 损伤

Abstract: Action of N-acetyl-L-cystein (NAC) for resisting damage of human skin fibroblast (HSF) induced by H2O2 was studied.Results of experiments for cell survival ratio and cell positive ratio showed that under low mass concentration (50－100 mg·L-1) of NAC,there is no obvious cell toxicity;and under mass concentration of 200~800 mg·L-1,cell proliferation is promoted;while under higher mass concentration (1 000 mg·L-1),the growth of HSF is inhibited obviously.Positive ratio of blank group,H2O2 group,H2O2 (500 μmol·L-1)+NAC (100 mg·L-1) group and H2O2 (500 μmol·L-1)+NAC (200 mg·L-1) group is 39.90%,69.57%,45.29% and 36.60% respectively.NAC can decrease the cell positive ratio obviously and resist the cell damage induced by H2O2.

Key words: additive of cosmetics, N-acetyl-L-cystein, human skin fibroblast, toxicity, damage

中图分类号: 

  • TQ658