高效液相色谱法同时测定葡萄柚提取物牙膏中6种黄酮类物质

doi:10.3969/j.issn.2097-2806.2025.07.015

摘要/Abstract

摘要： 建立了高效液相色谱法（HPLC）同时检测葡萄柚提取物牙膏中6种主要黄酮类物质（柚皮苷、橙皮苷、新橙皮苷、柚皮素、圣草次甙、橘皮素）的检测方法，流动相为甲醇和0.1%甲酸水溶液，牙膏提取溶剂为80%甲醇。方法学考察结果显示，精密度RSD范围为0.93%~1.42%，稳定性RSD范围为0.94%~1.42%，重复性RSD范围为1.14%~1.44%，所有RSD均<2.0%。精密度、稳定性和重复性良好。6种黄酮类物质在1~100 μg/mL质量浓度范围内呈良好的线性关系，相关系数为0.999 4~0.999 8。6种黄酮类物质的平均回收率范围为95.20%~104.57%，RSD范围为0.51%~2.06%。应用本研究检测方法可准确检测葡萄柚提取物牙膏中6种黄酮类物质的含量，对于葡萄柚提取物牙膏的质量控制具有参考价值。

关键词: 高效液相色谱法, 牙膏, 葡萄柚提取物, 黄酮类物质, 同时检测, 质量控制

Abstract:

In this study, a high performance liquid chromatography （HPLC） method was established to simultaneously detect six major flavonoids （naringin, hesperidin, neohesperidin, naringenin, eriocitrin, and tangeretin） in grapefruit extract toothpaste. The mobile phase was methanol and 0.1% formic acid aqueous solution, and the toothpaste extraction solvent was 80% methanol. The results of the methodological investigation show that the precision RSD ranges from 0.93% to 1.42%, the stability RSD ranges from 0.94% to 1.42%, and the repeatability RSD ranges from 1.14% to 1.44%, and all RSDs are less than 2.0%. The six flavonoids show a good linear relationship in the mass concentration range of 1-100 μg/mL, with a correlation coefficient of 0.999 4-0.999 8. The average recovery rate of the six flavonoids ranges from 95.20% to 104.57%, and the RSD ranges from 0.51% to 2.06%. The detection method in this study can accurately detect the content of the six flavonoids in grapefruit extract toothpaste, which has a reference value for the quality control of grapefruit extract toothpaste.

Key words: HPLC, toothpaste, grapefruit extract, flavonoids, simultaneous detection, quality control

中图分类号:

TQ658

靳晓婷, 李锦胜. 高效液相色谱法同时测定葡萄柚提取物牙膏中6种黄酮类物质[J]. 日用化学工业（中英文）, 2025, 55(7): 937-942.

Xiaoting Jin, Jinsheng Li. Simultaneous determination of 6 flavonoids in grapefruit extract toothpaste by high performance liquid chromatography[J]. China Surfactant Detergent & Cosmetics, 2025, 55(7): 937-942.

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黄酮类物质	加入量/（mg/g）	实测量/（mg/g）	回收率/%	RSD/%	平均回收率/%	RSD/%
柚皮苷	1	0.94	94.01	0.70	97.97	0.82
	10	10.33	103.26	1.57
	50	48.32	96.64	0.28
橙皮苷	1	1.01	100.54	0.87	95.20	1.07
	10	8.64	86.36	1.63
	50	49.35	98.70	0.90
新橙皮苷	1	1.080	108.1	0.51	104.57	2.06
	10	10.38	103.75	0.77
	50	50.92	101.84	1.18
柚皮素	1	0.95	94.98	0.80	98.08	1.02
	10	9.34	93.41	1.60
	50	52.92	105.85	0.42
圣草次甙	1	0.98	97.62	1.38	101.11	0.51
	10	9.81	98.06	0.63
	50	53.82	107.64	1.93
橘皮素	1	1.01	101.18	1.70	102.78	0.83
	10	10.17	101.71	0.75
	50	52.72	105.44	1.56

黄酮类物质	含量/（μg/g）
黄酮类物质	葡萄柚提取物牙膏1	葡萄柚提取物牙膏2	葡萄柚提取物牙膏3	葡萄柚提取物牙膏4
柚皮苷	382.98	425.56	226.59	134.34
橙皮苷	115.66	164.93	214.24	163.69
新橙皮苷	74.26	129.66	128.41	147.50
柚皮素	214.89	331.10	15.14	226.85
圣草次甙	39.73	52.23	105.59	18.65
橘皮素	78.62	102.24	149.39	69.22