中药复方MHC-20的抗化脓性链球菌功效与作用机制探究

doi:10.3969/j.issn.2097-2806.2024.03.006

摘要/Abstract

摘要：

以化脓性链球菌（Streptococcus pyogenes）为研究对象，探究了MHC-20的抑菌性能及作用机制。通过微量肉汤稀释法以及常量稀释法测得MHC-20对化脓性链球菌的MIC值为31.25 μg/mL或0.31%，并且对金黄色葡萄球菌以及白色念珠菌等均有较强杀伤作用。瞬时杀菌活性结果表明：MHC-20在5 min时可以完全杀死化脓性链球菌。定量生物膜与疏水性测定结果表明：MHC-20能够极显著降低化脓性链球菌细胞表面疏水性（P<0.000 1）以及极显著抑制生物膜的形成（P<0.01）。细胞膜通透性测定结果表明：MHC-20作用下菌液相对电导率、碱性磷酸酶均上升、蛋白严重泄露，并且呈浓度依赖性。扫描电子显微镜结果表明：MHC-20对菌体形态有很强的破坏作用。最后，构建了小鼠化脓性链球菌皮肤感染模型，结果表明：复方MHC-20可以促进创口恢复，极显著抑制创口的细菌（P<0.01），5% MHC-20与20% MHC-20均极显著降低小鼠血清以及创口炎症因子IL-6表达（P<0.001），20% MHC-20极显著降低白细胞的表达（P<0.01），综上所述，MHC-20有望成为广谱抗菌剂以及抗化脓性链球菌皮肤感染的有效药物。

关键词: 化脓性链球菌, MHC-20, 中药材, 机制, 皮肤感染

Abstract:

The antimicrobial properties and mechanisms of a formula of Chinese medicinal plants （MHC-20） against Streptococcus pyogenes were studied. The MIC of MHC-20 against S. pyogenes was 31.25 μg/mL or 0.31% as measured by broth micro-or macro-dilution. MHC-20 was also found to have strong killing effects on Staphylococcus aureus, Escherichia coli and Candida albicans. The results of instantaneous bactericidal activity showed that MHC-20 was able to kill S. pyogenes completely in 5 min. It could also significantly reduce the cell surface hydrophobicity and inhibit the formation of biofilm. Cell membrane permeability test showed that MHC-20 could induce the increase of relative conductivity and alkaline phosphatase, as well as the protein leakage, in a concentration-dependent manner. The results of scanning electron microscopy further confirmed that MHC-20 had a strong destructive effect on cell morphology. Finally, a mouse model of cutaneous infection with S. pyogenes was established. It was shown that MHC-20 could promote wound healing and significantly inhibit the loading concentration of S. pyogenes （P<0.01）; 5% MHC-20 and 20% MHC-20 significantly decreased the expression of IL-6 in serum and tissue of the mice （P<0.001）. 20% MHC-20 significantly decreased the expression of leukocytes （P<0.01）. In conclusion, MHC-20 had the potential to be developed into a broad-spectrum antibiotic and an effective drug for cutaneous infection with S. pyogenes.

Key words: Streptococcus pyogenes, MHC-20, Chinese medicinal plant, mechanism, skin infection

中图分类号:

TQ658

胡翠翠, 周代红, 陈欣菀, 钟佳伶, 茆灿泉. 中药复方MHC-20的抗化脓性链球菌功效与作用机制探究[J]. 日用化学工业（中英文）, 2024, 54(3): 282-289.

Cuicui Hu, Daihong Zhou, Xinwan Chen, Jialing Zhong, Canquan Mao. Efficacies and mechanisms of a formula of Chinese medicinal plants （MHC-20） against Streptococcus pyogenes[J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 282-289.

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