蛋白酶在洗衣液中的稳定性改良

doi:10.3969/j.issn.1001-1803.2019.02.008

日用化学工业 ›› 2019, Vol. 49 ›› Issue (2): 103-107.doi: 10.3969/j.issn.1001-1803.2019.02.008

蛋白酶在洗衣液中的稳定性改良

吴美娜^1,²,李磊^1,²,夏咏梅^1,²(),刘湘^1,²,王海军^1,²

1.食品科学与技术国家重点实验室,江苏无锡 214122
2.江南大学化学与材料工程学院,江苏无锡 214122

收稿日期:2018-06-06 修回日期:2018-12-22 出版日期:2019-02-22 发布日期:2019-03-15
通讯作者: 夏咏梅
作者简介:吴美娜（1996-）,女,广东人,硕士研究生,电话：15251633816,E-mail：wumeina1111@163. com。
基金资助:
"十三五"国家科技支撑计划资助项目(2017YFB0308700)

Improvement of proteases stability in liquid laundry detergent

Mei-na WU^1,²,Lei LI^1,²,Yong-mei XIA^1,²(),Xiang LIU^1,²,Hai-jun WANG^1,²

1.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
2.School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China

Received:2018-06-06 Revised:2018-12-22 Online:2019-02-22 Published:2019-03-15
Contact: Yong-mei XIA

摘要/Abstract

摘要：

以硼砂为对照,考察了16种柠檬酸盐、多元醇及酯系列稳定剂,以及温度、pH、稳定剂用量对3种蛋白酶在洗衣液中稳定性的影响;同时基于洗衣液的主要成分考察了表面活性剂AES,AEO和APG等对蛋白酶活的影响。实验结果表明,柠檬酸盐尤其是柠檬酸铵钙的稳定效果最佳,APG0810和APG1214的稳定效果与硼砂接近。当蛋白酶用量为0.5%时,以柠檬酸铵钙-APG0810-APG1214三元复合体系稳定蛋白酶,含有Purafect 4000L和Purafect Prime 4000L的复合稳定剂体系在pH=7和25 ℃下的3周酶活保持率分别可达到84.3%和71.5%;在pH=8和37 ℃下的3周酶活保持率分别为47.2%和30.4%;硼砂体系pH=7和37 ℃下的3周酶活保持率分别为2.87%和13.5%。此外,AES会较大幅度地降低酶活,而非离子表面活性剂AEO₇和AEO₉对其酶活无显著影响,但AEO和AES的混合并不能降低AES对酶活的损伤,可以考虑在液体加酶洗涤剂中增加APG和AEO的使用。

关键词: 加酶液体洗涤剂, 蛋白酶, 酶活保持率, 稳定剂

Abstract:

With borax as control, the effects of 16 stabilizers of citrate, polyols and esters, temperature, pH and amount of stabilizer on the stability of three proteases in liquid laundry detergent were investigated, and the effects of surfactants AES, AEO and APG on the activity of protease were also investigated based on the main components of liquid laundry detergent. Results show that the stability of citrate, especially calcium ammonium citrate, is the best, and the stability of APG0810 and APG1214 is close to that of borax. When the amount of protease was 0.5%, the three-week activity retention rates of protease stabilized by calcium ammonium citrate-APG0810-APG1214 ternary complex stabilizer system, Purafect 4000L and Purafect Prime 4000L complex stabilizer at pH=7 and 25 ℃ were 84.3% and 71.5%, respectively; the three-week activity retention rates at pH=8 and 37 ℃ were 47.2% and 30.4%, respectively; and the three-week activity retention rates of protease stabilized by borax at pH=7 and 37 ℃ were 2.87% and 13.5%, respectively. In addition, AES can significantly reduce the enzyme activity, while non-ionic surfactants AEO₇ and AEO₉ did not. However, the mixing of AEO and AES cannot reduce the damage of AES to the enzyme activity. It can be considered to increase the use of APG and AEO in liquid enzyme-enhanced detergents.

Key words: enzyme-enhanced liquid detergent, protease, retention of enzyme activity, stabilizer

中图分类号:

TQ649

吴美娜,李磊,夏咏梅,刘湘,王海军. 蛋白酶在洗衣液中的稳定性改良[J]. 日用化学工业, 2019, 49(2): 103-107.

Mei-na WU,Lei LI,Yong-mei XIA,Xiang LIU,Hai-jun WANG. Improvement of proteases stability in liquid laundry detergent[J]. China Surfactant Detergent & Cosmetics, 2019, 49(2): 103-107.

图/表 7

图 1

表 1

表 2

表 3

图 2

图 3

参考文献 9

[1]	Gulmez C, Atakisi O, Dalginli K Y , et al. A novel detergent additive: Organic solvent and thermo-alkaline-stable recombinant subtilisin[J]. International Journal of Biological Macromolecules, 2018,108:436-443. doi: 10.1016/j.ijbiomac.2017.11.133
[2]	Sattar H, Aman A, Qader S A U . Agar-agar immobilization: An alternative approach for the entrapment of protease to improve the catalytic efficiency, thermal stability and recycling efficiency[J]. Int J Biol Macromol, 2018,111:917-922. doi: 10.1016/j.ijbiomac.2018.01.105
[3]	Ibrahim A S, Al-Salamah A A, El-Toni A M, et al. Enhancement of alkaline protease activity and stability via covalent immobilization onto hollow core-mesoporous shell silica nanospheres[J]. Int J Mol Sci, 2016,17(2) : 184-202. doi: 10.3390/ijms17020184
[4]	Russell G L, Britton L N . Use of certain alcohol ethoxylates to maintain protease stability in the presence of anionic surfactants[J]. Journal of Surfactants and Detergents, 2002,5(1) : 5-10. doi: 10.1007/s11743-002-0198-9
[5]	Chandrika K M, Edward W B, Song X Q, . Liquid detergent composition exhibiting enhanced α-amylase enzyme stability: US11195538[P]. 2009-08-25.
[6]	Zhang Hongyan, Zhang Qiang, Ma Qi , et al. Study on storage stability of dedicated enzymes used in liquid detergents[J]. Technology and Market, 2011 (6) : 5-6.
[7]	Rowe R I, Bouzan C, Nabili S , et al. The response of trout and zebrafish embryos to low and high boron concentrations is U-shaped[J]. Biological Trace Element Research, 1998,66(1) : 261-270. doi: 10.1007/BF02783142
[8]	Bustos-O E, Carvallo M, Hartley-B R , et al. Histopathological and histometrical assessment of boron exposure effects on mouse spermatogenesis[J]. Int J Morphol, 2007,25(4) : 919-925. doi: 10.4067/S0717-95022007000400039
[9]	Li Jing, Zhang Jian, Zhao Yongxiang . Interaction between calcium ions and alkaline protease and study of its mechanism[J]. China Surfactant Detergent & Cosmetics, 2018,48(1) : 1-7.

编号	A	B	C	D
编号	w （柠檬酸铵钙）/%	w（APG0810） / %	w（APG1214） / %	w （蛋白酶） / %
1	0.5	0.5	0.5	0.5
2	1	1	1	1
3	2	2	2	2

实验号	酶活保持率/%
实验号	0 h	24 h	48 h	96 h	1周	4周
空白	100	10.2	0
1	100	96.5	84.8	73.6	63.4
2	100	84.1	83.9	72.5	56.8
3	100	78.3	68.8	59.4	45.4
4	100	76.9	75.1	64.6	44.6
5	100	85.1	76.9	73.2	70.9	42.3
6	100	85.5	74.2	65.6	50.0
7	100	99.1	83.4	71.8	60.8
8	100	83.3	71.7	60.6	43.3
9	100	91.7	82.5	76.2	69.7	43.7
硼砂	100	35.4	1.68
柠檬酸铵钙	100	67.3

实验号	酶活保持率/%
实验号	0 h	24 h	48 h	96 h	1周	4周
1	100	82.5	77.2	65.7	45.8	28.1
2	100	71.6	51.2	30.9
3	100	54.0	36.1	21.3
4	100	57.7	40.7	20.7
5	100	93.2	81.1	63.8	56.8	32.7
6	100	66.5	53.9	39.1
7	100	77.9	65.4	49.2
8	100	72.2	53.9	30.4
9	100	93.7	84.2	67.3	55.1	33.5
柠檬酸铵钙	100	98.7	84.0	71.8	55.8	37.7

蛋白酶名称	稳定剂	储存温度/℃	体系pH	酶活保持率/%
蛋白酶名称	稳定剂	储存温度/℃	体系pH	0 h	24 h	1周	2周	3周
Purafect 4000L	复合稳定剂	37	8	100	81.9	68.3	51.0	47.2
		25	8	100	89.4	84.9	82.3	76.2
		37	7	100	84.1	65.2	50.1	47.1
		25	7	100	94.8	97.7	86.5	84.3
	硼砂	37	7	100	81.8	35.6	11.0	2.87
	硼砂	25	7	100	94.9	91.2	72.4	65.7
Purafect Prime 4000L	复合稳定剂	37	8	100	84.2	60.3	47.6	30.4
		25	8	100	85.9	81.0	73.6	50.3
		37	7	100	93.5	62.1	43.9	42.0
		25	7	100	95.0	80.7	80.2	71.5
	硼砂	37	7	100	80.8	41.7	25.7	13.5
	硼砂	25	7	100	96.5	84.6	79.5	68.4

[1]	王雪娇, 杜丽娟, 徐元喜. 液体洗涤蛋白酶稳定剂筛选方法开发[J]. 日用化学工业（中英文）, 2023, 53(7): 796-801.
[2]	Ranjit Kaur Bhogal, 陈旭斌, David Messenger, 宫哲慧, 孙佳凝, 皮瑛瑛, 顾佳音. 吡罗克酮乙醇胺盐的蛋白酶抑制作用及与硫酸锌复配的防脱发功效研究[J]. 日用化学工业（中英文）, 2023, 53(4): 390-397.
[3]	唐靖惠,王毅,马淑骅,孙娅楠,贺改英,何忠梅. 鹿茸提取物对UVB诱导光老化小鼠皮肤的保护作用[J]. 日用化学工业, 2022, 52(9): 969-974.
[4]	李子宜,刘彦,张召,吴惠勤. 制备一种包埋木瓜蛋白酶生物膜材料并应用于美白护肤品[J]. 日用化学工业, 2022, 52(3): 294-301.
[5]	王朋辉,王伟贤,曾晖,芮泽宝. 碱性蛋白酶洗涤稳定性提高的研究进展[J]. 日用化学工业, 2022, 52(2): 180-189.
[6]	刘春媛,樊雨梅,何婕,周广运,苏宁,廖峰. 驴乳延缓皮肤衰老的研究[J]. 日用化学工业, 2021, 51(8): 754-760.
[7]	郭雨飞,张剑,于文. 洗涤用蛋白酶PB92的分子动力学模拟[J]. 日用化学工业, 2021, 51(4): 299-305.
[8]	王朋辉,王伟贤,杨涛,曾晖,芮泽宝,李东华,黄平. 碱性蛋白酶洗涤性能的影响因素[J]. 日用化学工业, 2021, 51(11): 1109-1117.
[9]	李慧,蒋丹丹,DOS SANTOS Morgan,章漳,蒋耀权. 紫茉莉花提取物促进胶原蛋白功能化的功效研究[J]. 日用化学工业, 2019, 49(8): 526-530.
[10]	贺改英,陈迪,沈丽. 中草药祛痘复方对痤疮致病菌和外泌酶的影响[J]. 日用化学工业, 2018, 48(7): 403-407.
[11]	李静, 张剑, 赵永祥. 钙离子与碱性蛋白酶的相互作用及机理研究[J]. 日用化学工业, 2018, 48(1): 1-7.
[12]	李静, 张剑, 赵永祥. 金属离子对蛋白酶作用的研究进展[J]. 日用化学工业, 2017, 47(6): 345-351.
[13]	王晶, 张剑, 张越. 碱性蛋白酶的空气/水界面行为研究[J]. 日用化学工业, 2015, 45(9): 490-494.
[14]	李林, 许元妹, 卢丽霞, 赵顺祥. 稳定剂对洗涤剂中过碳酸钠漂白活化体系的影响[J]. 日用化学工业, 2015, 45(7): 385-388.
[15]	康林霞, 张剑, 秦洁, 张晟. 碱性蛋白酶在液体洗涤剂中的稳定性[J]. 日用化学工业, 2014, 44(3): 139-142.

蛋白酶在洗衣液中的稳定性改良

Improvement of proteases stability in liquid laundry detergent

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 9

相关文章 15

Metrics

本文评价

推荐阅读 10