乙基纤维素-海藻酸钠/壳聚糖微凝胶的制备及表征

doi:10.3969/j.issn.1001-1803.2022.12.001

摘要/Abstract

摘要：

为解决常见微凝胶体系释放过快、缓释效果差的问题，通过乳液法，一步制备了一种新型乙基纤维素包裹的海藻酸钠/壳聚糖微凝胶。对相同水相配比下制备出的水凝胶和微凝胶进行扫描电子显微镜观察、红外光谱、热失重效果、接触角分析和溶胀性能检测，证明了乙基纤维素包覆的海藻酸钠/壳聚糖微凝胶的成功制备。通过对小鼠单核巨噬细胞（RAW264.7）的细胞活性检测发现，微凝胶具有良好的生物相容性能，在100 mg/L的质量浓度下没有明显细胞毒性。利用模型药物甲基蓝考察了不同药物含量、乙基纤维素含量、海藻酸钠和壳聚糖含量、酸钙比（n（GDL） ∶n（EDTA-Ca））和钙含量对制备出的微凝胶粒径和包封率的影响，制备出微凝胶的包封率可达到67.1%。最后，对该体系的体外释放效果进行评价，结果显示该体系的持续释放时间长达15 h，且具有一定的pH敏感性。

关键词: 微凝胶, 核壳结构, 缓释, 生物相容性

Abstract:

The porous nature of microgels allows soluble active ingredients to diffuse into or out of the particles. As a result, the release performance of microgels usually can only sustain for 5 hours, which greatly limits their application. Therefore, to solve the problems of the poorly sustained release performance of common microgel systems, a new type of （ethyl cellulose）-coated sodium alginate/chitosan （EC-Alginate/CS） microgel was prepared. An one-step emulsification method was employed to generate the new type of EC-Alginate/CS core-shell microgel. Ultra-depth microscope, scanning electron microscope （SEM）, infrared spectroscopy （FT IR）, thermogravimetric analysis （TGA）, water contact angle （WCA） and swelling ratio were used to characterize the matrix hydrogels and microgels prepared at the same water phase composition to optimize the EC-Alginate/CS core-shell microgels. The cytotoxicity experiments showed that the cell viability of mouse mononuclear macrophages （RAW264.7） at 0.1 g/L was 89.3%, which could prove that the system had good biocompatibility at this concentration. It was found that the encapsulation efficiency （EE） of microgels could achieve 67.1%. The cumulative release effect of the model drug methyl blue （MB） showed that the system could achieve a sustained release of 15 h with a certain pH sensitivity. This study provided theoretical support and application reference for the bioavailable sustained-release system for water-soluble active substances, and it might be further applied to daily chemical products such as cosmetics and laundry detergents.

Key words: microgel, core-shell structure, sustained release, biocompatibility

中图分类号:

O648

齐佳悦,毛燚,陈林倩,王靖,杨成,孙亚娟. 乙基纤维素-海藻酸钠/壳聚糖微凝胶的制备及表征[J]. 日用化学工业（中英文）, 2022, 52(12): 1259-1268.

Qi Jiayue,Mao Yi,Chen Linqian,Wang Jing,Yang Cheng,Sun Yajuan. Preparation and characterization of ethyl cellulose-sodium alginate/chitosan microgels[J]. China Surfactant Detergent & Cosmetics, 2022, 52(12): 1259-1268.

图/表 13

参考文献 35

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MB/%（w/V）	Diameter/μm	EE/%
0.01	6.0±1.5	23.4±3.7
0.1	5.8±1.2	25.5±3.2
1	5.5±0.9	8.2±1.3

EC/%（w/V）	Diameter/μm	EE/%
0.5	9.5±1.9	19.8±1.8
1	5.8±1.2	25.5±3.2
2	4.9±1.0	28.6±0.8

Alginate/%（w/V）	Chitosan/%（w/V）	Diameter/μm	EE/%
0.5	0.5	7.3±2.1	12.7±1.3
1	1	5.8±1.2	19.8±1.3
2	2	6.0±1.7	25.5±3.2

GDL/Ca ratio	Diameter/μm	EE/%
2	4.5±1.2	67.1±3.3
10	5.8±1.2	25.5±3.2
20	8.0±2.0	14.7±0.9

EDTA-Ca/%（w/V）	Diameter/μm	EE/%
0.2	4.6±1.1	17.5±2.8
0.4	5.8±1.2	25.5±3.2
0.6	5.0±1.3	32.5±0.4