滨蒿提取物在化妆品领域的多种用途

doi:10.3969/j.issn.2097-2806.2026.01.013

摘要/Abstract

摘要：

采用超声辅助乙醇提取法制备滨蒿提取物，其中对羟基苯乙酮含量>10%。通过斑马鱼模型、巨噬细胞炎症模型及抑菌率评价法，系统评估滨蒿提取物不同剂量下的美白、舒缓及抑菌作用，并结合鸡胚尿囊膜（HET - CAM）眼刺激模型评价其有效剂量下的刺激性。结果显示：在美白功效方面，0.2%和0.4%提取物显著抑制斑马鱼胚胎头部黑色素生成（P<0.05），效果优于0.1%烟酰胺；0.4%提取物使LPS诱导的TNF-α分泌量降低42.33%，符合化妆品舒缓标准；0.2%~0.4%滨蒿提取物对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌的最小抑菌浓度（MIC90）均≤1%，抑菌效果显著。0.4%的滨蒿提取物在HET-CAM模拟眼刺激中ES=0，无眼刺激性。本研究证实滨蒿提取物在0.2%~0.4%质量分数下兼具美白、舒缓、抑菌三重功效，且安全性良好，符合化妆品原料安全标准，为开发多功能天然化妆品提供了科学依据。

关键词: 滨蒿提取物, 美白, 舒缓, 抑菌, 安全性评价

Abstract:

This study prepared Artemisia scoparia extract using ultrasound-assisted ethanol extraction, with a p-hydroxyacetophenone content over 10%. The whitening, soothing, and antibacterial effects of the extract at different doses were systematically evaluated using zebrafish models, macrophage inflammation models, and antibacterial rate assays, combined with the hen's egg chorioallantoic membrane（HET-CAM）test to assess ocular irritation at effective doses. The results demonstrate that, for whitening efficacy, 0.2%-0.4% extract significantly inhibits melanin generation in zebrafish embryos（P<0.05）, outperforming 0.1% niacinamide（P<0.05）. The 0.4% extract reduces LPS-induced TNF-α secretion by 42.33%, meeting cosmetic soothing standards. The minimum inhibitory concentrations（MIC90）of 0.2%-0.4% Artemisia scoparia extract against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli are all ≤1%, indicating a significant antibacterial activity. Additionally, the 0.4% extract shows no ocular irritation （ES=0） in the HET-CAM test. This study confirms that Artemisia scoparia extract at 0.2%-0.4% concentrations exhibits triple efficacy in whitening, soothing, and antibacterial effects, with excellent safety profiles compliant with cosmetic ingredient standards, providing a scientific basis for developing multifunctional natural cosmetics.

Key words: Artemisia scoparia extract, whitening, soothing, antibacterial, safety evaluation

中图分类号:

TQ658

李凰, 王媛, 刘胜茂, 佘振南, 陈真. 滨蒿提取物在化妆品领域的多种用途[J]. 日用化学工业（中英文）, 2026, 56(1): 107-113.

Huang Li, Yuan Wang, Shengmao Liu, Zhennan She, Zhen Chen. Multiple applications of Artemisia scoparia extract in the cosmetic field[J]. China Surfactant Detergent & Cosmetics, 2026, 56(1): 107-113.

图/表 5

图1

图2

图3

表1

图4

参考文献 23

[1]	尹德航, 施乐洋, 叶雨涵, 等. 天然产物及其有效成分在化妆品领域的研究与开发[J]. 化学试剂, 2022, 44(7): 960-967.
[2]	刘敏, 邢书霞, 张慧文, 等. 已使用化妆品原料目录(2021年版)制修订内容介绍[J]. 香料香精化妆品, 2023(1): 18-25, 41.
[3]	黄丽平, 许远航, 邓敏贞, 等. 茵陈的化学成分、药理作用机制与临床应用研究进展[J]. 天然产物研究与开发, 2021, 33(4): 676-690.
[4]	Kim M J, Mohamed E A, Kim D S, et al. Inhibitory effects and underlying mechanisms of Artemisia capillaris essential oil on melanogenesis in the B16F10 cell line[J]. Molecular Medicine Reports, 2022, 25(4): 1-12. doi: 10.3892/mmr
[5]	祝茗, 张开梅, 丁燕. 茵陈蒿中滨蒿內酯及东莨菪素抗氧化活性的评价[J]. 沈阳药科大学学报, 2016, 33(11): 889-893.
[6]	Khan F A, Khan N M, Ahmad S, et al. Phytochemical profiling, antioxidant, antimicrobial and cholinesterase inhibitory effects of essential oils isolated from the leaves of Artemisia scoparia and Artemisia absinthium[J]. Pharmaceuticals, 2022, 15(10): 1221-1221. doi: 10.3390/ph15101221
[7]	刘玉萍, 邱小玉, 刘烨, 等. 茵陈的药理作用研究进展[J]. 中草药, 2019, 50(9): 2235-2241.
[8]	Prusty J S, Kumar A. Coumarins: antifungal effectiveness and future therapeutic scope[J]. Molecular Diversity, 2020, 24(4): 1367-1383. doi: 10.1007/s11030-019-09992-x
[9]	Im D S. Pro-resolving effect of ginsenosides as an anti-inflammatory mechanism of Panax ginseng[J]. Biomolecules, 2020, 10(3): 444. doi: 10.3390/biom10030444
[10]	Nie Y, Liu Q, Zhang W, et al. Ursolic acid reverses liver fibrosis by inhibiting NOX4/NLRP3 inflammasome pathways and bacterial dysbiosis[J]. Gut Microbes, 2021, 13(1): 1972746. doi: 10.1080/19490976.2021.1972746
[11]	崔生辉, 陈怡文, 路勇. 化妆品防腐剂使用与皮肤微生物的关系[J]. 卫生研究, 2022, 51(1): 153-156.
[12]	阿如汗, 斯日古冷, 辛颖. 滨蒿的化学成分、药理作用及质量标准研究概况[J]. 中国民族医药杂志, 2020, 26(8): 48-52.
[13]	王媛, 刘胜茂, 郭春宝, 等. 一种决滨蒿提取物及其制备方法和应用: CN 118005495 A[P]. 2024-05-10.
[14]	中国药典委员会. [S]. 北京: 中国医药科技出版社, 2020.
[15]	上海日用化学品行业协会. [S]. 国标委全国团体标准信息平台, 2019.
[16]	上海日用化学品行业协会. [S]. 国标委全国团体标准信息平台, 2021.
[17]	上海日用化学品行业协会. [S]. 国标委全国团体标准信息平台, 2018.
[18]	Yeh T M, Sung W W, Shih W L. Antibacterial and antibiofilm effects of Camellia oleifera seed dreg extract and its application in cosmetics[J]. Journal of Cosmetic Dermatology, 2024, 23(3): 1055-1065. doi: 10.1111/jocd.v23.3
[19]	Ha H Y, Lee H Y, Seo C S, et al. Artemisia capillaris inhibits atopic dermatitis-like skin lesions in Dermatophagoides farinae-sensitized Nc/Nga mice[J]. BMC Complementary and Alternative Medicine, 2014, 14(1): 100. doi: 10.1186/1472-6882-14-100
[20]	Gendrisch F, Esser P R, Schempp C M, et al. Luteolin as a modulator of skin aging and inflammation[J]. BioFactors (Oxford, England), 2020, 47(2): 170-180. doi: 10.1002/biof.v47.2
[21]	Bano R, Arsalan A. In vitro antibacterial susceptibility of different brands of oral levofloxacin 250 mg tablet against Staphylococcus aureus and Escherichia coli[J]. Pakistan Journal of Pharmaceutical Sciences, 2018, 31(6): 2529-2535.
[22]	Cushnie T P, Lamb A J. Antimicrobial activity of flavonoids[J]. International Journal of Antimicrobial Agents, 2005, 26(5): 343-356. doi: 10.1016/j.ijantimicag.2005.09.002 pmid: 16323269
[23]	Zhao H, Yu F, Wang C, et al. The impacts of sodium lauroyl sarcosinate in facial cleanser on facial skin microbiome and lipidome[J]. Journal of Cosmetic Dermatology, 2024, 23(4): 1351-1359. doi: 10.1111/jocd.v23.4

测试物质量分数/%	抑菌率/%
	金黄色葡萄球菌		铜绿假单胞菌		大肠杆菌
	滨蒿提取物	阳性对照组	滨蒿提取物	阳性对照组	滨蒿提取物	阳性对照组
0.2	84.52±0.34^*	92.48±0.47	82.99±0.91^*	92.91±0.32	78.24±0.93^**	84.43±0.89
0.3	85.49±0.44^**	94.39±0.25	85.00±0.33^**	93.03±0.60	85.44±0.40^**	95.06±0.30
0.4	86.39±0.63^**	94.56±0.33	85.43±0.98^**	94.01±0.77	86.14±0.31^**	95.20±0.95
0.5	89.87±0.94^**	96.11±0.95	95.17±0.63^**	98.79±0.17	87.20±0.74^**	97.52±0.95
1.0	97.91±0.15^**	99.10±0.72	98.40±0.53^**	99.55±0.39	97.47±0.44^**	99.88±0.10