神经酰胺复合物对光损伤3D全层皮肤模型的作用研究

doi:10.3969/j.issn.1001-1803.2023.01.007

摘要/Abstract

摘要：

通过重建光损伤3D全层皮肤模型，利用石蜡切片免疫组化、免疫荧光染色等办法，观察样本细胞、组织形态，检测模型细胞外基质Ⅰ型胶原蛋白，表皮屏障蛋白FLG、CLDN-1、LCE-A1以及相关应激酶的含量变化，评估神经酰胺复合物对光损伤的成年皮肤真表皮层的影响。实验发现神经酰胺复合物可以显著缓解因紫外造成的表皮分化紊乱、胶原蛋白流失、屏障蛋白降低和皮肤应激防御反应，减少皮肤因紫外辐照产生的一系列负面影响，对皮肤光损伤具有预防修护作用。

关键词: 神经酰胺复合物, 光损伤, 3D全层皮肤模型, 预防修护

Abstract:

In this study, a 3D full-thickness skin model with UV-induced damage was reconstructed to evaluated the effects of ceramide complex. Paraffin section immunohistochemistry and immunofluorescence staining were used to observe the morphology of sample cells and tissues, detect the content changes of ECM protein such type I collagen, a number of late differentiation markers: filaggrin, claudin-1, LCE-1A as well as stress-related enzymes in the model. The results show that ceramide complex can significantly alleviate epidermal differentiation disorder, collagen loss, barrier damage and skin stress defense response caused by UV irradiation. Therefore, the complex reduces a series of negative effects caused by ultraviolet radiation on skin, which has a preventive and repair effect on skin from UV damage.

Key words: ceramide complex, UV-induced damage, 3D full-thickness skin model, preventive maintenance

中图分类号:

TQ658

蔡卓, 卢媛, 汪冰洁, 刘俏苑. 神经酰胺复合物对光损伤3D全层皮肤模型的作用研究[J]. 日用化学工业（中英文）, 2023, 53(1): 47-53.

Cai Zhuo, Lu Yuan, Wang Bingjie, Liu Qiaoyuan. Efficacy evaluation of ceramide complex on 3D full-thickness skin model under UV-induction[J]. China Surfactant Detergent & Cosmetics, 2023, 53(1): 47-53.

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