采用DPRA替代方法评价3种香料的皮肤致敏性

doi:10.3969/j.issn.1001-1803.2021.05.011

日用化学工业 ›› 2021, Vol. 51 ›› Issue (5): 438-442.doi: 10.3969/j.issn.1001-1803.2021.05.011

采用DPRA替代方法评价3种香料的皮肤致敏性

杨婷婷¹(),易路遥¹(),王绎²,李杰¹,吉伟佳¹,章红¹

1.江西省药品检验检测研究院国家药品监督管理局中成药质量评价重点实验室江西省药品与医疗器械质量工程技术研究中心,江西南昌 330029
2.国科健康控股（江西）有限公司,江西南昌 330029

收稿日期:2020-08-09 修回日期:2021-05-05 出版日期:2021-05-22 发布日期:2021-05-21
通讯作者: 易路遥
作者简介:杨婷婷（1990-）,女,工程师,电话：15970476585,E-mail： 273332923@qq.com。
基金资助:
江西省药品监督管理局科研项目(2019JS03)

Evaluation of skin sensitization of three perfumes by using DPRA alternative method

YANG Ting-ting¹(),YI Lu-yao¹(),WANG Yi²,LI Jie¹,JI Wei-jia¹,ZHANG Hong¹

1. Engineering Research Center of Drug and Medical Device Quality of Jiangxi Province, National Medical Products Administration Key Laboratory of Chinese Patent Medicine Quality Evaluation, Jiang Xi Institute for Drug Control, Nanchang, Jiangxi 330029, China
2. Cas Health Holding （Jiangxi） Co. LTD, Nanchang, Jiangxi 330029, China

Received:2020-08-09 Revised:2021-05-05 Online:2021-05-22 Published:2021-05-21
Contact: Lu-yao YI

摘要/Abstract

摘要：

采用直接肽反应试验（DPRA）对新铃兰醛、氯化苔黑醛和苔黑醛3种香料的致敏性进行评价。在本实验室建立DPRA方法,采用高效液相色谱仪测定新铃兰醛、氯化苔黑醛和苔黑醛及其他6种已知致敏性化合物的多肽消除率,并进行皮肤致敏性的预测判定。通过比较DPRA和小鼠局部淋巴结试验（LLNA）结果的一致性,判断方法的可行性,并最终判定3种香料的致敏性。结果表明,6种已知化合物的DPRA和LLNA致敏性结果一致,3种香料的DPRA预测结果均为阳性。DPRA方法操作简便,准确度高,可以作为皮肤致敏性评价的替代方法。

关键词: 直接肽反应试验, 皮肤致敏性, 香料, 新铃兰醛, 氯化苔黑醛, 苔黑醛

Abstract:

The sensitization of lyral, chloroatranol and atranol was evaluated by direct peptide reaction test （DPRA） . The DPRA method was established in our lab. The polypeptide elimination rate of lyral, chloroatranol, atranol and six other compounds with known sensitization were measured by HPLC, and the skin sensitization was then predicted. The feasibility of DPRA method was determined by comparing its consistency with LLNA results, and the sensitization of the three perfumes was finally determined. The sensitization results of the six known compounds as evaluated by DPRA and LLNA were consistent, and the DPRA predictions of the three perfumes were positive. The DPRA method was simple and accurate, and could be used as an alternative method for skin sensitization evaluation.

Key words: direct peptide reaction test （DPRA）, skin sensitization, perfume, lyral, chloroatranol, atranol

中图分类号:

TQ658

杨婷婷,易路遥,王绎,李杰,吉伟佳,章红. 采用DPRA替代方法评价3种香料的皮肤致敏性[J]. 日用化学工业, 2021, 51(5): 438-442.

YANG Ting-ting,YI Lu-yao,WANG Yi,LI Jie,JI Wei-jia,ZHANG Hong. Evaluation of skin sensitization of three perfumes by using DPRA alternative method[J]. China Surfactant Detergent & Cosmetics, 2021, 51(5): 438-442.

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参考文献 14

[1]	Saint-Mezard P, Krasteva M, Chavagnac C, et al. Afferent and efferent phases of allergic contact dermatitis (ACD) can be induced after a single skin contact with haptens: evidence using a mouse model of primary ACD[J]. The Journal of Investigative Dermatology, 2003,120:641-647. doi: 10.1046/j.1523-1747.2003.12093.x
[2]	Peiser M, Tralau T, Heidler J, et al. Allergic contact dermatitis: epidemiology, molecular mechanisms, in vitro methods and regulatory aspects[J]. Cellular and Molecular Life Sciences, 2012,18:763-781.
[3]	Cahill J, Williams J D, Matheson M C, et al. Occupational contact dermatitis: a review of 18 years of data from an occupational dermatology clinic in Australia[J]. Canberra ACT: Safe Work Australia, 2012.
[4]	Kimber I, Dearman R J, Scholes E W, et al. The local lymph node assay: developments and applications[J]. Toxicology, 1994,93:13-31. pmid: 7974502
[5]	Zhang Yao, Ma Kunjun, Xiong Jing, et al. Modified DPRA based on UPLC for predicting skin sensitization of chemicals[J]. Daily Chemical Science, 2017,40(11): 1-4.
[6]	OECD. Test No. 442C: In chemico skin sensitisation: direct peptide reactivity assay (DPRA)[S]. Paris: Organization for Economic Cooperation and Development, 2015.
[7]	OECD. Test No. 442D: In vitro skin sensitisation: ARE-Nrf2 luciferase test method[S]. Paris: Organization for Economic Cooperation and Development, 2015.
[8]	OECD. Test No. 442E: In vitro skin sensitisation: human cell line activity (h-CLAT)[S]. Paris: Organization for Economic Cooperation and Development, 2016.
[9]	Fagen Zhang, Tim Erskine, Joanna Klapacz, et al. A highly sensitive and selective high pressure liquid chromatography with tandem mass spectrometry (HPLC/MS-MS) method for the direct peptide reactivity assay (DPRA)[J]. Journal of Pharmacological and Toxicological Methods, 2018,94:1-15. doi: S1056-8719(18)30649-X pmid: 30099091
[10]	Wang Ying, Wu Yue. Establishment and application of non-animal test method for skin sensitization (DPRA)[J]. China Surfactant Detergent & Cosmetics, 2017,47(2): 90-95, 108.
[11]	Gen Xue, Wang Xiaobing, Wei Xia, et al. Application of new in silico model in initial screening of skin sensitizing potential of cosmetic ingredients[J]. Flavour Fragrance Cosmetics, 2020 (2): 27-32, 35.
[12]	Gen Xue, Zhang Lei, Xu wenyu, et al. Evaluation of skin sensitizing potential of p-aminophenol by in silico and in chemico techniques[J]. Chin. J. Pharm. Anal., 2019,39(8): 1495-1500.
[13]	Hu Peili, Liu Shibo, Zhang Huiliang, et al. Development of a peptide reactivity assay for screening chemical allergens[J]. Canceration Aberration Mutation, 2018,30(2): 150-154.
[14]	Ding Shixuan, Li Xiaolin, Qu Li, et al. Application of direct peptide reactivity assay on cosmetics[J]. Daily Chemical Science, 2018,41(11): 19-24.

半胱氨酸和赖氨酸消耗百分比的均值（x）/%	反应等级	DPRA预测结果
0 ≤x≤6.38	无或轻微反应	阴性
6.38<x≤22.62	弱反应	阳性
22.62<x≤42.47	中度反应	阳性
42.47<x≤100	强反应	阳性

受试物	半胱氨酸多肽消耗率/%	赖氨酸多肽消耗率/%	多肽平均消耗率/%	反应等级	DPRA 预测结果	LLNA 参考结果
甲醛	26.34±0.76	100.00±0.00	63.2	强反应	阳性	强致敏物
6-甲基香豆素	0.49±0.08	3.49±0.53	2.0	无	阴性	非致敏物
对苯二胺	100.00±0.00	5.02±0.35	52.5	强反应	阳性	强致敏物
对氨基苯甲酸	0	0	0	无	阴性	非致敏物
异丙醇	0	0	0	无	阴性	非致敏物
肉桂醛	100.00±0.00	46.11±4.05	73.1	强反应	阳性	强致敏物
新铃兰醛	100.00±0.00	5.90±1.14	53.0	强反应	阳性	/
氯化苔黑醛	62.29±13.54	86.85±5.19	74.6	强反应	阳性	/
苔黑醛	3.09±0.43	21.69±1.49	12.4	弱反应	阳性	/

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[13]	刘显明, 汤小芳. 6,7-二甲氧基香豆素的合成[J]. 日用化学工业, 2014, 44(9): 517-520.
[14]	沈德立, 穆旻, 何昕, 刘玉平, 田红玉, 孙宝国. 玫瑰麝香酯的合成研究[J]. 日用化学工业, 2014, 44(9): 508-511.
[15]	陈娟, 尹学琼. 香料香精的安全性及防范措施与评价标准[J]. 日用化学工业, 2014, 44(2): 100-104.

采用DPRA替代方法评价3种香料的皮肤致敏性

Evaluation of skin sensitization of three perfumes by using DPRA alternative method

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t/min	φ（流动相A）/%	φ（流动相B）/%	流速/（mL·min^-1）
0	90	10	1.00
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15	10	90	1.00
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