杜仲预防UVB致人成纤维细胞光老化的作用及基因水平的机制探讨

doi:10.13218/j.cnki.csdc.2016.01.007

摘要/Abstract

摘要： 以UVB辐射体外培养的人成纤维细胞,探讨了杜仲乙醇提取物乙酸乙酯萃取部分(简称杜仲提取物)预防人成纤维细胞光老化的作用,并利用基因芯片对其可能的作用机制进行了探索。实验结果表明,杜仲提取物可以减少UVB对人成纤维细胞活性的影响,具有较好的预防光老化的作用,且基因芯片结果提示这种防护作用可能与提高细胞的抗氧化、DNA修复、抗凋亡有关,同时可能还具有一定促进细胞增殖和预防黑素细胞瘤的作用。

关键词: 化妆品添加剂, 杜仲, 光老化, UVB, 基因芯片

Abstract: Based upon test with irradiation of UVB on human skin fibroblast cells (HSFs) cultivated in vitro,the action of ethyl acetate extract from ethanol extract of Eucommia ulmoides (hereinafter refers to as the Eucommia ulmoides extract) for prevention of photo-aging damage of HSFs was explored;and its possible action mechanism was searched by utilizing cDNA microarray.Experimental results showed that the Eucommia ulmoides extract can reduce the effect of UVB on the HSBs and displays rather good action for preventing photo-aging damage.Further,the cDNA microarray test results demonstrated that possibly,such prevention action may correlates with the enhancement of antioxidation capacity of the HSFs,the DNA repairing system,and the capacity for suppression of apoptosis.Meanwhile,it may have the action for promoting cell proliferation and preventing the growth of melanoma.

Key words: additives of cosmetics, Eucommia ulmoides, photo-aging, UVB, cDNA microarray

中图分类号:

TQ658

陈默,赵亚,孙懿. 杜仲预防UVB致人成纤维细胞光老化的作用及基因水平的机制探讨[J]. 日用化学工业, 2016, 46(1): 35-38.

CHEN Mo,ZHAO Ya,SUN Yi. Action of Eucommia ulmoides extract for prevention of photo-aging damage of human skin fibroblast cells induced by UVB and exploration of its mechanism at genetic level[J]. China Surfactant Detergent & Cosmetics, 2016, 46(1): 35-38.

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