基于聚集诱导发光现象检测十二烷基三甲基溴化铵

doi:10.3969/j.issn.1001-1803.2021.12.001

摘要/Abstract

摘要：

阳离子表面活性剂对土壤和水系微生物具有明显的毒性,快捷而灵敏的阳离子表面活性剂分析检测方法是监控阳离子表面活性剂排放的重要工具,而利用聚集诱导发光（AIE）现象分析检测表面活性剂是一种新兴的快捷方法。但是,尚未见有基于AIE现象分析阳离子表面活性剂的报导。为此,利用一种水溶性的AIE探针1,1,2,2-四[4-（3-磺酸丙氧基）苯基]乙烯盐（TSTPE）并以十二烷基三甲基溴化铵（DTAB）为模型物,建立了基于AIE现象检测水体中DTAB的“turn-on”荧光分析方法。结果表明：游离的TSTPE在水中只有微弱的荧光发射;与DTAB以静电相互作用结合后,其荧光发射强度（F）显著增强,并且随DTAB浓度（c）增加而进一步地增强;当TSTPE的浓度为4.5×10^-5 mol/L时,F与c的线性响应范围约为1.05×10^-4~1.7×10^-4 mol/L,检测限约为5.7×10^-7 mol/L;在pH为3~10范围内TSTPE-DTAB混合体系的荧光发射具有较好的稳定性,NaCl,KCl和NaNO₃三种无机盐对TSTPE-DTAB混合体系的荧光发射干扰较小,在模拟水样中DTAB的回收率不低于95%。

关键词: 荧光探针, 聚集诱导发光, 检测, 阳离子表面活性剂

Abstract:

Cationic surfactants have obvious toxicity to microorganisms in soil and water. Methods for fast and sensitive analysis and detection of cationic surfactants are important tools for monitoring the discharge of cationic surfactants. Aggregation-induced emission （AIE） phenomenon analysis is an emerging and fast method to detect surfactants. However, the analysis of cationic surfactants based on AIE phenomenon has not been reported. Therefore, a “turn-on” fluorescence method for detecting dodecyltrimethylammonium bromide （DTAB） in water was established by using a water-soluble AIE probe, 1, 1, 2, 2-tetrakis[4-（3-sulfonatopropoxyl）phenyl] ethylene salt （TSTPE）, and using DTAB as the model. The results showed that free TSTPE was only weakly emissive in water; after binding with DTAB by electrostatic interaction, its fluorescence emission intensity （F） was significantly enhanced and was further increased with the increase of DTAB concentration （c）. When the concentration of TSTPE was 4.5×10 ^-5 mol/L, the linear response range of F vs. c was around 1.05×10 ^-4~1.7×10^-4 mol/L, and the detection limit was approximately 5.7×10^-7mol/L. The fluorescence emission of TSTPE-DTAB mixed system had good stability in the pH range of 3~10; three inorganic salts, NaCl, KCl and NaNO₃, had little interference with the fluorescence emission of the TSTPE-DTAB mixture, and the recovery rate of DTAB in simulated water samples was not less than 95%.

Key words: fluorescent probe, aggregation-induced emission, detection, cationic surfactant

中图分类号:

TQ423

孙庆,方银军,刘雪锋. 基于聚集诱导发光现象检测十二烷基三甲基溴化铵[J]. 日用化学工业, 2021, 51(12): 1163-1170.

Sun Qing,Fang Yinjun,Liu Xuefeng. Detection of dodecyltrimethylammonium bromide based on aggregation-induced emission phenomenon[J]. China Surfactant Detergent & Cosmetics, 2021, 51(12): 1163-1170.

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样品	加标量/（mol·L^-1）	加标后测定值/（mol·L^-1）	回收率/%	相对标准偏差/%
超纯水	1.2×10^-4	（1.195 2±0.003 2）×10^-4	99.60±0.27	0.27
	1.4×10^-4	（1.389 7±0.002 0）×10^-4	99.19±0.14	0.14
	1.6×10^-4	（1.587 4±0.004 4）×10^-4	99.21±0.27	0.27
模拟水	1.2×10^-4	（1.179 9±0.003 9）×10^-4	98.32±0.33	0.33
	1.4×10^-4	（1.367 2±0.005 0）×10^-4	97.66±0.37	0.37
	1.6×10^-4	（1.540 9±0.007 3）×10^-4	96.30±0.48	0.48