疏水改性透明质酸的制备及性能

doi:10.3969/j.issn.1001-1803.2020.11.007

摘要/Abstract

摘要：

通过化学反应将胆固醇（CHO）引入透明质酸（HA）的分子结构中,获得疏水改性透明质酸（HACHO）。利用核磁氢谱和红外光谱对合成产物结构进行了表征。使用透射电子显微镜（TEM）、荧光光谱以及流变仪等对HACHO的临界聚集浓度、在水中的自组装行为及流变行为等进行了研究。采用MTT法评价了HACHO的体外细胞毒性。在此基础上,将HACHO应用于制备双凝胶乳液,考察其稳定乳液的性能。结果表明,HACHO具有一定的界面活性,较好的生物相容性,且能在水相体系中形成较紧致的网络结构,能更好地稳定双凝胶乳液,在化妆品领域具有广阔的应用前景。

关键词: 透明质酸, 胆固醇, 疏水改性, 流变行为, 双凝胶乳液

Abstract:

Cholesterol （CHO） was grafted onto the molecular structure of hyaluronic acid （HA） through a chemical reaction to obtain a modified product HACHO. ¹H NMR and FT-IR were used to characterize the structure of the modified product. Transmission electron microscope （TEM）, fluorescence spectrum and rheometer were used to study the critical aggregation concentration, self-assembly behavior and rheological behavior of HACHO in water. The cell viability of the modified product HACHO was evaluated by the MTT assay. On this basis, HACHO was used to prepare bi-gel emulsion, and the stability of the emulsion was investigated. The results showed that, HACHO had interfacial activity and good biocompatibility, and could form a network structure in aqueous phase to stabilize the bi-gel emulsion. Therefore, HACHO showed a broad application prospect in the field of cosmetics.

Key words: hyaluronic acid, cholesterol, hydrophobic modification, rheological behavior, bi-gel emulsion

中图分类号:

TQ658

石梦琳,刘力嘉,尚亚卓,刘洪来. 疏水改性透明质酸的制备及性能[J]. 日用化学工业, 2020, 50(11): 776-782.

SHI Meng-lin,LIU Li-jia,SHANG Ya-zhuo,LIU Hong-lai. Preparation and properties of hydrophobically modified hyaluronic acid[J]. China Surfactant Detergent & Cosmetics, 2020, 50(11): 776-782.

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