QuEChERS-HPLC-MS/MS法测定4种化妆品基质中15种硝基咪唑类非法添加药物的基质效应研究

doi:10.3969/j.issn.1001-1803.2020.04.011

Abstract

Abstract:

A QuEChERS coupled with high performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS） method was developed for the detection of 15 nitroimidazole drugs illegally added in 4 kinds of cosmetic matrices. The matrix effects of 15 nitroimidazole drugs in water aqua, powder, lotion and cream, together with the influences of matrix type, matrix mass concentration and drug mass concentration on the matrix effects were discussed. The result show that the 4 kinds of cosmetic have different degrees of matrix effects on 15 nitroimidazole drugs, and most of them are matrix inhibitory effects, which are impacted by matrix type, matrix mass concentration and drug mass concentration. Therefore, it’s crucial to take matrix effect into consideration during the detection of the nitroimidazoles illegally added in cosmetic products. For some matrices with weak matrix effects, such as water aqua and powder, the matrix effects can be ignored according to actual needs. For emulsions and cream substrates with significant matrix effects, it is recommended to use a matrix-matched standard curve for quantitative analysis to ensure the accuracy of the test results.

Key words: matrix effect, HPLC-MS/MS, cosmetics, nitroimidazoles, illegally added

CLC Number:

TQ658

CHEN Cen,YAN Ling-qi,CHENG Qiao-yuan. Research of matrix effect of 15 nitroimidazole drugs illegally added in 4 kinds of cosmetic matrices by QuEChERS-HPLC-MS/MS methods[J].China Surfactant Detergent & Cosmetics, 2020, 50(4): 275-281.

Figures/Tables 9

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References 19

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序号	化合物	保留时间/min	母离子（m/z）	子离子（m/z）		碰撞能量/V
1	4-HMZ	3.07	114.2	68.2*,	84.2	15,12
2	MNI	4.15	128.2	82.1*,	111.1	15,15
3	MNZOH	4.29	188.2	123.2*,	126.2	15,14
4	MNZ	5.33	172.2	82.1*,	128.2	20,15
5	HMMNI	5.37	158.2	140.2*,	55.2	15,15
6	DMZ	5.95	142.2	96.2*,	81.2	15,20
7	RNZ	6.81	201.2	140.2*,	55.2	12,20
8	TerNDZ	7.00	186.2	82.2*,	128.2	25,15
9	NBI	7.46	164.2	118.2*,	91.1	18,30
10	SNZ	7.94	186.2	128.2*,	82.1	15,20
11	CMNI	8.81	162.2	116.1*,	145.1	17,15
12	TNZ	9.26	248.2	121.2*,	128.2	15,20
13	IPZOH	10.45	186.2	168.2*,	122.2	15,20
14	ONZ	10.88	220.2	82.1*,	128.2	25,15
15	IPZ	13.00	170.2	124.2*,	109.2	17,24

化合物	ME
	水剂		散粉		乳液		膏霜
	MC	DC	MC	DC	MC	DC	MC	DC
4-HMZ	0.84~0.96	0.66~0.98	0.81~0.85	0.83~0.97	0.42~0.63	0.54~0.66	0.86~0.91	0.89~0.95
MNI	0.89~1.00	0.97~1.07	0.93~0.96	0.90~1.05	0.39~0.44	0.40~0.51	0.94~0.96	0.95~0.98
MNZOH	0.90~0.92	0.89~1.07	0.90~0.93	0.77~0.92	0.57~0.61	0.65~0.72	0.91~0.93	0.97~1.01
MNZ	0.96~1.01	0.96~1.05	1.06~1.09	0.90~1.09	0.41~0.48	0.44~0.55	0.85~0.95	0.90~1.13
HMMNI	0.95~0.98	0.90~0.98	0.98~1.04	0.86~1.12	0.74~0.78	0.78~0.90	0.95~0.98	0.93~1.04
DMZ	0.97~1.01	0.93~1.07	0.98~1.02	0.97~1.08	0.91~0.96	0.95~0.97	0.92~0.95	0.94~1.05
RNZ	0.97~1.02	0.94~1.05	0.93~0.96	0.93~1.11	0.88~0.93	0.95~1.07	0.90~0.98	0.98~1.10
TerNDZ	0.95~1.02	0.92~1.04	0.95~1.01	0.94~1.15	0.93~0.95	0.92~0.97	0.95~0.98	0.99~1.21
NBI	0.92~0.97	0.93~1.08	0.87~0.90	0.90~1.01	0.93~0.95	0.95~1.01	0.92~0.96	0.98~1.07
SNZ	0.95~0.99	0.93~1.10	0.98~1.02	0.96~1.08	0.92~0.95	0.95~0.98	0.94~0.99	0.99~1.13
CMNI	0.96~0.98	0.95~1.10	0.98~1.02	0.91~1.17	0.89~0.92	0.91~1.09	0.96~1.02	0.91~1.07
TNZ	0.85~0.93	0.78~0.93	0.97~0.99	0.89~1.07	0.85~0.90	0.77~0.94	0.80~0.82	0.81~0.98
IPZOH	0.85~0.87	0.85~0.94	0.96~0.97	0.99~1.08	0.91~0.94	0.93~0.99	0.90~0.95	0.97~1.12
ONZ	0.96~1.00	1.00~1.21	0.99~1.02	0.87~1.17	1.01~1.07	1.06~1.17	0.65~0.74	0.71~0.80
IPZ	0.92~0.94	0.94~1.00	0.98~1.01	0.95~1.10	0.51~0.59	0.59~0.62	0.87~0.94	0.91~0.95

Research of matrix effect of 15 nitroimidazole drugs illegally added in 4 kinds of cosmetic matrices by QuEChERS-HPLC-MS/MS methods

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