高效液相色谱法测定化妆品中的氨甲环酸

doi:10.3969/j.issn.1001-1803.2019.11.011

Abstract

Abstract:

A high performance liquid chromatography （HPLC） method for the determination of tranexamic acid in whitening cosmetics was established. The tranexamic acid in cosmetic samples was extracted directly with 5% methanol solution. The separation was performed on a Shiseido CAPCELL PAK C₁₈ UG120 column （4.6 mm×250 mm×5 μm）, with the mobile phase of methanol and 0.05 mol/L KH₂PO₄-0.2% H₃PO₄ solution. The flow rate was 1.0 mL/min and detection wavelength was 210 nm. The detection results show good linearity within the range of 5-100 mg/L （r>0.999）. The limit of detection （LOD） is 10.7-22.2 μg/g and the limit of quantitation （LOQ） is 28.9-48.0 μg/g in the cosmetics matrix. The recoveries range from 95.23% to 110.40%, and the RSD range is 1.24%-3.59%（n=6）. The intraday stability is good, and the peak area RSD value is 0.026% within 24 h. The method is simple and reliable, and can be used for the determination of tranexamic acid in whitening cosmetics.

Key words: cosmetics, tranexamic acid, high performance liquid chromatography

CLC Number:

TQ658

DAI Jing,GAO Jia-min,CAO Jin,WANG Gang-li. Determination of tranexamic acid in whitening cosmetics by high performance liquid chromatography[J].China Surfactant Detergent & Cosmetics, 2019, 49(11): 760-763.

Figures/Tables 5

Tab. 1

Tab. 2

Tab. 3

Fig. 1

Fig. 2

References 9

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时间	峰面积
0	0.172
1	0.174
2	0.183
4	0.176
8	0.171
12	0.175
24	0.169
RSD/%	0.026

化妆品基质	检出限/（μg·g^-1）	定量限/（μg·g^-1）
爽肤水	13.6	33.6
乳液	12.0	28.9
凝胶	10.7	42.1
面膜	12.6	30.8
面霜	22.2	48.0

化妆品基质	添加水平/ （mg·L^-1）	加标回收率/%						平均回收率/%	RSD/%
化妆品基质	添加水平/ （mg·L^-1）	1	2	3	4	5	6	平均回收率/%	RSD/%
爽肤水	10	95.79	100.15	96.92	101.47	96.92	100.34	98.60	2.37
	50	101.33	105.52	102.98	99.43	98.56	103.25	101.85	2.55
	100	97.69	103.20	98.89	97.29	99.37	100.28	99.45	2.15
面膜	10	100.33	101.47	94.65	104.88	97.83	98.02	99.53	3.54
	50	107.33	105.33	106.20	107.33	107.35	103.28	106.14	1.53
	100	107.62	103.54	106.07	105.83	106.77	107.24	106.18	1.30
凝胶	10	106.02	100.36	102.56	104.98	102.33	103.59	103.31	1.96
	50	108.24	110.92	110.06	113.24	108.92	111.03	110.40	1.61
	100	103.66	108.78	102.88	106.62	107.52	106.02	105.91	2.14
乳液	10	102.60	98.97	100.33	102.65	105.69	99.23	101.58	2.53
	50	95.97	96.88	99.60	95.51	98.24	92.79	96.50	2.44
	100	103.47	104.25	99.15	102.79	104.16	103.13	102.82	1.83
面霜	10	104.88	103.33	98.32	99.86	96.56	99.35	100.38	3.12
	50	95.97	90.74	97.70	100.21	93.47	93.26	95.23	3.59
	100	102.04	99.94	99.23	102.42	100.18	101.13	100.82	1.24

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Determination of tranexamic acid in whitening cosmetics by high performance liquid chromatography

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