Abstract: HPLC method for determination of 15 kinds of sun screener used in cosmetics specified in Cosmetic Health Norm (2007 edition) was optimized and improved.The sample was extracted with a mixed solution composed of V(methanol)∶V(tetrahydrofuran,THF)∶V(water)∶V(0.067% perchloric acid)=250∶450∶300∶0.2 and separated with methanol-THF-0.067% perchloric acid as mobile phase with gradient elution on Kromasil C₁₈ analytical column,at flow rate of 1 mL/min,and detector wavelength of 311 nm.The method shows good linear relationship in conventionally proper mass concentration range;the spiked recoveries are between 85.6%-115.9%;the detectable limit is within 0.8-12.5 ng.

Key words: cosmetics, sun screener, high performance liquid chromatography