LC-MS/MS法测定化妆品中迪高替尼等7种特应性皮炎治疗药物

doi:10.3969/j.issn.2097-2806.2026.06.012

Abstract

Abstract:

A liquid chromatography-tandem mass spectrometry （LC-MS/MS） method has been established for the simultaneous determination of seven atopic dermatitis treatment drugs in 4 different cosmetic matrices. Following vortex dispersion and ultrasonic extraction with the test sample using 80% methanol, the mixture was filtered through a 0.22 μm PTFE membrane. Chromatographic separation of the filtrate was achieved on a Kinetex^® C₁₈ （2.6 μm×2.1 mm×100 mm） column, utilizing a gradient elution system consisting of 0.1% formic acid in water and acetonitrile as the mobile phase. The determination of target compounds was performed using an electrospray ionization source （ESI） in multiple reaction monitoring mode （MRM）. The results show that the seven atopic dermatitis treatment drugs exhibit good linear relationships within their respective linear ranges （r>0.997）, with limits of detection （LODs） ranging from 0.8 to 171 μg/kg. The recovery rates of the seven drugs in the 4 cosmetics matrices range from 86.5% to 109.7%, with relative standard deviations （RSD） of 0.13% to 5.4%. This method is simple, rapid, and provides reliable qualitative and quantitative analysis, making it highly suitable for the determination of these drugs in cosmetics. Furthermore, it can provide valuable technical support for cosmetic risk monitoring and standard revisions.

Key words: cosmetics, atopic dermatitis, delgocitinib, high performance liquid chromatography-tandem mass spectrometry

CLC Number:

TQ658

Ceyi Fu, Yanyan Su, Ying Liang, Wenjia Xu, Qiuping Nong. Simultaneous determination of 7 atopic dermatitis treatment drugs including delgocitinib in cosmetics by LC-MS/MS[J].China Surfactant Detergent & Cosmetics, 2026, 56(6): 798-803.

Figures/Tables 4

Tab.1

Fig.1

Tab.2

Tab.3

References 20

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序号	化合物名称/ （扫描方式）	保留时间/min	母离子（m/z）	子离子（m/z）	锥孔电压/V	碰撞能量/eV
1	迪高替尼/ （+）	1.77	311.3	110.1^*	20	31
1	迪高替尼/ （+）	1.77	311.3	244.2	20	19
2	芦可替尼/ （+）	2.75	307.3	186.0^*	20	30
2	芦可替尼/ （+）	2.75	307.3	159.0	20	38
3	乌帕替尼/ （+）	2.33	381.4	256.1^*	20	29
3	乌帕替尼/ （+）	2.33	381.4	213.1	20	46
4	巴瑞替尼/ （+）	2.39	372.3	251.1^*	20	31
4	巴瑞替尼/ （+）	2.39	372.3	186.0	20	44
5	地法米司特/ （+）	4.93	447.3	149.0^*	20	20
5	地法米司特/ （+）	4.93	447.3	121.0	20	40
6	罗氟司特/ （-）	4.36	402.2	138.0^*	30	37
6	罗氟司特/ （-）	4.36	402.2	158.0	20	33
7	本维莫德/（-）	4.28	253.1	169.2^*	39	29
7	本维莫德/（-）	4.28	253.1	210.8	39	21

化合物名称	基质类型	线性范围/ （ng/mL）	线性方程	r	检出限/ （μg/kg）	定量限/ （μg/kg）
迪高替尼	水剂	0.16~41	y=9.62×10³x-7.66×10	0.999 6	8	26
	乳液		y=1.37×10⁴x+1.72×10²	1.000 0	8	26
	膏霜		y=9.82×10³x-8.01×10	0.999 7	8	26
	凝胶		y=6.78×10³x+1.96×10²	0.999 8	8	26
芦可替尼	水剂	0.031~8.1	y=7.22×10⁴x+7.07×10²	0.999 7	2	5
	乳液		y=7.37×10⁴x-1.86×10²	1.000 0	2	5
	膏霜		y=7.74×10⁴x+1.04×10³	0.999 8	2	5
	凝胶		y=6.98×10⁴x-8.17×10²	0.999 8	2	5
乌帕替尼	水剂	0.084~21	y=3.78×10⁴x-2.47×10²	0.999 6	4	14
	乳液		y=3.62×10⁴x-3.89×10³	0.999 8	4	14
	膏霜		y=3.80×10⁴x-3.17×10²	1.000 0	4	14
	凝胶		y=3.44×10⁴x-1.21×10³	0.999 9	4	14
巴瑞替尼	水剂	0.089~23	y=1.62×10⁴x+6.17×10²	0.999 6	4	15
	乳液		y=1.50×10⁴x-6.43×10²	1.000 0	4	15
	膏霜		y=1.65×10⁴x-8.29×10²	0.999 9	4	15
	凝胶		y=1.48×10⁴x-6.61×10²	0.999 9	4	15
地法米司特	水剂	0.016~4.1	y=1.93×10⁵x+2.61×10³	0.999 8	0.8	2.6
	乳液		y=2.13×10⁵x-2.17×10³	0.999 8	0.8	2.6
	膏霜		y=2.04×10⁵x+1.60×10³	0.999 9	0.8	2.6
	凝胶		y=1.82×10⁵x+1.15×10³	0.999 7	0.8	2.6
罗氟司特	水剂	2.1~332	y=4.52×10x+3.07×10²	0.998 3	57	190
	乳液		y=4.04×10x+1.90×10²	0.999 7	57	190
	膏霜		y=4.56×10x+4.01×10²	0.997 8	57	190
	凝胶		y=4.46×10x+3.88×10²	0.998 2	57	190
本维莫德	水剂	3.4~874	y=3.13×10²x-4.02×10²	0.999 6	171	569
	乳液		y=2.53×10²x-1.53×10³	0.999 6	171	569
	膏霜		y=2.87×10²x-4.15×10²	0.999 9	171	569
	凝胶		y=2.89×10²x-1.47×10³	0.999 8	171	569

化合物名称	水剂		乳液		膏霜		凝胶
化合物名称	平均回收率/%	RSD/%	平均回收率/%	RSD/%	平均回收率/%	RSD/%	平均回收率/%	RSD/%
迪高替尼	86.5 95.0 98.5	2.2 0.62 0.81	98.5 107.2 106.9	0.51 1.2 0.43	104.3 107.7 107.5	3.1 1.3 1.0	99.6 90.1 102.1	1.1 1.7 0.58
芦可替尼	87.9 96.7 100.7	0.13 1.7 1.2	100.1 105.0 106.9	3.0 0.72 0.69	105.7 108.1 107.5	1.1 0.39 0.67	103.3 88.9 102.9	1.9 0.70 0.49
乌帕替尼	89.5 97.3 102.0	2.6 1.9 1.6	104.5 103.5 104.5	1.5 1.7 1.5	108.1 106.8 108.3	0.60 1.6 0.89	104.6 88.6 104.6	1.2 1.9 4.4
巴瑞替尼	87.1 97.7 100.9	1.4 2.7 0.56	101.5 104.2 104.6	2.3 1.9 0.47	108.8 108.6 109.7	0.71 1.1 0.46	105.0 91.0 103.1	2.7 0.71 0.34
地法米司特	87.2 97.1 100.6	2.7 1.1 0.47	103.9 108.4 107.8	3.7 1.3 1.5	102.8 105.2 107.9	2.6 1.4 0.91	98.6 87.4 103.2	2.1 1.5 0.49
罗氟司特	87.5 107.8 99.2	2.2 1.3 0.86	101.7 106.5 92.4	5.4 1.5 3.0	100.4 109.1 98.3	1.9 1.1 2.5	99.1 108.5 91.8	3.6 0.52 3.8
本维莫德	93.7 99.9 97.9	4.8 1.7 0.53	104.2 104.9 107.5	0.96 2.4 1.1	93.7 104.1 100.0	1.6 2.8 0.69	96.4 92.7 91.3	2.2 0.89 1.6

Simultaneous determination of 7 atopic dermatitis treatment drugs including delgocitinib in cosmetics by LC-MS/MS

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